Molecular data mining to improve antibody-based detection of Grapevine leafroll-associated virus 1 (GLRaV-1)

•83 new complete CP gene sequences were obtained for GLRaV-1.•Conserved AA sequences suitable for antibody production were identified.•A monospecific IgG (Mono-IgG) was commercially produced against a 14 AA peptide.•The Mono-IgG was validated by in situ immunoassay and tissue print immunoblotting. T...

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Veröffentlicht in:	Journal of virological methods 2013-12, Vol.194 (1-2), p.258-270
Hauptverfasser:	Esteves, Filipa, Teixeira Santos, Margarida, Eiras-Dias, José Eduardo, Fonseca, Filomena
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture - methods alkaline phosphatase Antibodies Antibodies, Viral - isolation & purification Capsid protein Capsid Proteins - genetics Capsid Proteins - immunology certification Closteroviridae - genetics Closteroviridae - immunology Closteroviridae - isolation & purification coat proteins Conserved Sequence Data Mining enzyme-linked immunosorbent assay European Union fluorescein genetic databases Grapevine leafroll-associated virus 1 immunization Immunoassay - methods immunoblotting In situ immunoassay Intra-isolate genetic structure Molecular variability nucleotide sequences Plant Diseases - virology polyclonal antibodies polymerase chain reaction propagation materials reverse transcription RNA, Viral - genetics Sequence Analysis, DNA Tissue print immunoblotting vines Virology - methods
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