Quantification of Sesquiterpene Lactones in Parthenium hyterophorous by Normal-Phase HPLC
This paper describes the development of a normal-phase liquid chromatography ultraviolet-diode array detection method for the simultaneous quantification of parthenin and coronopilin in the leaves and flowers of Parthenium hysterophorous. The compounds were analyzed on a Merck Si60 silica column (5...
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| Veröffentlicht in: | Journal of chromatographic science 2013-11, Vol.51 (10), p.950-953 |
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| container_title | Journal of chromatographic science |
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| creator | Chib, Renu Shah, Bhahwal Ali Andotra, Samar Singh Bharadwaj, Vikram Gupta, Rajinder Kumar Taneja, Subhash Chandra Khajuria, Ravi Kant |
| description | This paper describes the development of a normal-phase liquid chromatography ultraviolet-diode array detection method for the simultaneous quantification of parthenin and coronopilin in the leaves and flowers of Parthenium hysterophorous. The compounds were analyzed on a Merck Si60 silica column (5 µm, 250 × 4 mm) using an isocratic 15:85 mixture of isopropyl alcohol and hexane. The calibration curves resulting from the reference compounds in the concentration range of 200-2,000 ng exhibited acceptable linearity (r > 0.999). The method was developed to study the levels of parthenin and coronopilin in the leaves and flowers of P. hysterophorous collected during different seasons, and the method was validated by analyzing the spiked samples. |
| doi_str_mv | 10.1093/chromsci/bms195 |
| format | Article |
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The compounds were analyzed on a Merck Si60 silica column (5 µm, 250 × 4 mm) using an isocratic 15:85 mixture of isopropyl alcohol and hexane. The calibration curves resulting from the reference compounds in the concentration range of 200-2,000 ng exhibited acceptable linearity (r > 0.999). The method was developed to study the levels of parthenin and coronopilin in the leaves and flowers of P. hysterophorous collected during different seasons, and the method was validated by analyzing the spiked samples.</description><identifier>ISSN: 0021-9665</identifier><identifier>EISSN: 1945-239X</identifier><identifier>DOI: 10.1093/chromsci/bms195</identifier><identifier>PMID: 23456568</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Asteraceae - chemistry ; Azulenes - analysis ; Azulenes - chemistry ; Azulenes - isolation & purification ; Chromatography, High Pressure Liquid - methods ; Flowers ; Limit of Detection ; Linear Models ; Plant Leaves ; Reproducibility of Results ; Sesquiterpenes - analysis ; Sesquiterpenes - chemistry ; Sesquiterpenes - isolation & purification</subject><ispartof>Journal of chromatographic science, 2013-11, Vol.51 (10), p.950-953</ispartof><rights>The Author [2013]. 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The compounds were analyzed on a Merck Si60 silica column (5 µm, 250 × 4 mm) using an isocratic 15:85 mixture of isopropyl alcohol and hexane. The calibration curves resulting from the reference compounds in the concentration range of 200-2,000 ng exhibited acceptable linearity (r > 0.999). The method was developed to study the levels of parthenin and coronopilin in the leaves and flowers of P. hysterophorous collected during different seasons, and the method was validated by analyzing the spiked samples.</description><subject>Asteraceae - chemistry</subject><subject>Azulenes - analysis</subject><subject>Azulenes - chemistry</subject><subject>Azulenes - isolation & purification</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Flowers</subject><subject>Limit of Detection</subject><subject>Linear Models</subject><subject>Plant Leaves</subject><subject>Reproducibility of Results</subject><subject>Sesquiterpenes - analysis</subject><subject>Sesquiterpenes - chemistry</subject><subject>Sesquiterpenes - isolation & purification</subject><issn>0021-9665</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFLwzAUgIMobk7P3iRHEeqSpmmXowx1wtCJCnoqafJCI21Tk_awf29HN6-eHg--9_H4ELqk5JYSweaq9K4Oys6LOlDBj9CUioRHMROfx2hKSEwjkaZ8gs5C-N6tdMFP0SRmCU95upiir9deNp01VsnOugY7g98g_PS2A99CA3gtVecaCNg2eCN9V0Jj-xqX2wFwbem86wMutvjZ-VpW0aaUAfBqs16eoxMjqwAX-zlDHw_378tVtH55fFrerSPFMtZFRlGhgWkjtMoSJReFLGhKM6mUZIRQDiAE1zxWTMZcS11IQ4TSJiZEU1Bshq5Hb-vdTw-hy2sbFFSVbGD4LadJIrKMchoP6HxElXcheDB5620t_TanJN_1zA8987HncHG1l_dFDfqPPwQcgJsRcH37r-0X26yFkA</recordid><startdate>201311</startdate><enddate>201311</enddate><creator>Chib, Renu</creator><creator>Shah, Bhahwal Ali</creator><creator>Andotra, Samar Singh</creator><creator>Bharadwaj, Vikram</creator><creator>Gupta, Rajinder Kumar</creator><creator>Taneja, Subhash Chandra</creator><creator>Khajuria, Ravi Kant</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201311</creationdate><title>Quantification of Sesquiterpene Lactones in Parthenium hyterophorous by Normal-Phase HPLC</title><author>Chib, Renu ; Shah, Bhahwal Ali ; Andotra, Samar Singh ; Bharadwaj, Vikram ; Gupta, Rajinder Kumar ; Taneja, Subhash Chandra ; Khajuria, Ravi Kant</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-fc19de3df9dc74ca8bab1617acca30015ee995d52c3a25dadbaf09cdf200d1ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Asteraceae - chemistry</topic><topic>Azulenes - analysis</topic><topic>Azulenes - chemistry</topic><topic>Azulenes - isolation & purification</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Flowers</topic><topic>Limit of Detection</topic><topic>Linear Models</topic><topic>Plant Leaves</topic><topic>Reproducibility of Results</topic><topic>Sesquiterpenes - analysis</topic><topic>Sesquiterpenes - chemistry</topic><topic>Sesquiterpenes - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chib, Renu</creatorcontrib><creatorcontrib>Shah, Bhahwal Ali</creatorcontrib><creatorcontrib>Andotra, Samar Singh</creatorcontrib><creatorcontrib>Bharadwaj, Vikram</creatorcontrib><creatorcontrib>Gupta, Rajinder Kumar</creatorcontrib><creatorcontrib>Taneja, Subhash Chandra</creatorcontrib><creatorcontrib>Khajuria, Ravi Kant</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chib, Renu</au><au>Shah, Bhahwal Ali</au><au>Andotra, Samar Singh</au><au>Bharadwaj, Vikram</au><au>Gupta, Rajinder Kumar</au><au>Taneja, Subhash Chandra</au><au>Khajuria, Ravi Kant</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Sesquiterpene Lactones in Parthenium hyterophorous by Normal-Phase HPLC</atitle><jtitle>Journal of chromatographic science</jtitle><addtitle>J Chromatogr Sci</addtitle><date>2013-11</date><risdate>2013</risdate><volume>51</volume><issue>10</issue><spage>950</spage><epage>953</epage><pages>950-953</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><abstract>This paper describes the development of a normal-phase liquid chromatography ultraviolet-diode array detection method for the simultaneous quantification of parthenin and coronopilin in the leaves and flowers of Parthenium hysterophorous. 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| subjects | Asteraceae - chemistry Azulenes - analysis Azulenes - chemistry Azulenes - isolation & purification Chromatography, High Pressure Liquid - methods Flowers Limit of Detection Linear Models Plant Leaves Reproducibility of Results Sesquiterpenes - analysis Sesquiterpenes - chemistry Sesquiterpenes - isolation & purification |
| title | Quantification of Sesquiterpene Lactones in Parthenium hyterophorous by Normal-Phase HPLC |
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