Production of gynogenic haploids of Hordeum vulgare L
Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase...
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Veröffentlicht in: | Plant cell reports 1993, Vol.12 (3), p.139-143 |
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creator | CASTILLO, A. M CISTUE, L |
description | Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of callus formation and the number of green plants produced was possible with the addition of 90 g/l sucrose and 100 g/l coconut water. The stage of development of the ovaries at the time of culture was critical; the largest number of plants being produced by ovaries from flowers at the trinucleate stage of pollen. |
doi_str_mv | 10.1007/BF00239094 |
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The stage of development of the ovaries at the time of culture was critical; the largest number of plants being produced by ovaries from flowers at the trinucleate stage of pollen.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/BF00239094</identifier><identifier>PMID: 24196850</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Anther and ovule culture, utilization for haploid production ; Biological and medical sciences ; Biotechnology ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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M</creatorcontrib><creatorcontrib>CISTUE, L</creatorcontrib><title>Production of gynogenic haploids of Hordeum vulgare L</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of callus formation and the number of green plants produced was possible with the addition of 90 g/l sucrose and 100 g/l coconut water. The stage of development of the ovaries at the time of culture was critical; the largest number of plants being produced by ovaries from flowers at the trinucleate stage of pollen.</description><subject>Anther and ovule culture, utilization for haploid production</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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Technologies</topic><topic>Plant cells and fungal cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CASTILLO, A. M</creatorcontrib><creatorcontrib>CISTUE, L</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CASTILLO, A. 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The stage of development of the ovaries at the time of culture was critical; the largest number of plants being produced by ovaries from flowers at the trinucleate stage of pollen.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>24196850</pmid><doi>10.1007/BF00239094</doi><tpages>5</tpages></addata></record> |
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subjects | Anther and ovule culture, utilization for haploid production Biological and medical sciences Biotechnology Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Plant cells and fungal cells |
title | Production of gynogenic haploids of Hordeum vulgare L |
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