Establishment of hairy root cultures of Linum flavum producing the lignan 5-methoxypodophyllotoxin
Hairy root cultures were induced from leaf explants of Linum flavum by infection with Agrobacterium rhizogenes. The transformed nature of tissue was confirmed by the production of opines. The cultures produced 1.5 to 3.5% of the lignan 5-methoxypodophyllotoxin (5-MPT) on a dry weight basis, which wa...
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| Veröffentlicht in: | Plant cell reports 1993-05, Vol.12 (7-8), p.474-477 |
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| container_title | Plant cell reports |
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| creator | OOSTDAM, A MOL, J. N. M VAN DER PLAS, L. H. W |
| description | Hairy root cultures were induced from leaf explants of Linum flavum by infection with Agrobacterium rhizogenes. The transformed nature of tissue was confirmed by the production of opines. The cultures produced 1.5 to 3.5% of the lignan 5-methoxypodophyllotoxin (5-MPT) on a dry weight basis, which was 2 to 5 times higher than the 5-MPT content in untransformed root cultures and 5 to 12 times higher than in L. flavum cell suspensions. The 5-MPT production as a function of time was up to four times higher than that in cell suspensions. |
| doi_str_mv | 10.1007/BF00234715 |
| format | Article |
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The 5-MPT production as a function of time was up to four times higher than that in cell suspensions.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass culture</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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Technologies</topic><topic>Plant cells and fungal cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OOSTDAM, A</creatorcontrib><creatorcontrib>MOL, J. N. M</creatorcontrib><creatorcontrib>VAN DER PLAS, L. H. W</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OOSTDAM, A</au><au>MOL, J. N. M</au><au>VAN DER PLAS, L. H. 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| subjects | Biological and medical sciences Biotechnology Establishment of new cell lines, improvement of cultural methods, mass culture Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Plant cells and fungal cells |
| title | Establishment of hairy root cultures of Linum flavum producing the lignan 5-methoxypodophyllotoxin |
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