Construction of a designer chromosome in tobacco
The tobacco (Nicotiana tabacum L.) breeding line NC 152 is a doubled haploid that possesses an addition chromosome from Nicotiana africana (Merxm. and Buttler). A gene on this chromosome confers potyvirus resistance (PotyR). Our objective was to use the addition chromosome as a base on which to cons...
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| Veröffentlicht in: | Theoretical and applied genetics 1994-02, Vol.87 (7), p.837-842 |
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| container_title | Theoretical and applied genetics |
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| creator | Campbell, K.G. (Ohio State Univ., Wooster (USA). Agronomy Dept. Ohio Agricultural Research and Development Center) Wernsman, E.A Fitzmurice, W.P Burns, J.A |
| description | The tobacco (Nicotiana tabacum L.) breeding line NC 152 is a doubled haploid that possesses an addition chromosome from Nicotiana africana (Merxm. and Buttler). A gene on this chromosome confers potyvirus resistance (PotyR). Our objective was to use the addition chromosome as a base on which to construct a designer chromosome containing a foreign gene linkage package. A mutant dhfr gene conferring resistance to methotrexate (Mtx) was inserted into NC 152-haploid (n=25) leaf tissue via Agrobacterium tumefaciens-mediated transformation. After chromosome doubling, 135 NC 152dhfr transformants (2n=50) were pollinated with the potyvirus-susceptible (PotyS) cultivar 'McNair 944' (2n=48). Linkage analysis was performed in the BC1 generation. Two transformants, NC 152dhfr-996 and NC 152dhfr-1517, exhibited complete linkage between Mtx resistance (MtxR) and PotyR. Segregants from these two transformants which were MtxR and PotyR possessed 49 chromosomes, while Mtx sensitive (MtxS) and PotyS progeny possessed 48 chromosomes. Eighty percent of the NC 152dhfr transformants transmitted the dhfr gene as one locus. Other foreign genes can be directed to the addition chromosome through transformation followed by selection for single loci with linkage to PotyR or MtxR. The integrity of both the foreign-gene linkage package and the rest of the genome will be maintained because recombination between the N. africiana and the N. tabacum chromosomes has not been observed. |
| doi_str_mv | 10.1007/BF00221136 |
| format | Article |
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(Ohio State Univ., Wooster (USA). Agronomy Dept. Ohio Agricultural Research and Development Center) ; Wernsman, E.A ; Fitzmurice, W.P ; Burns, J.A</creator><creatorcontrib>Campbell, K.G. (Ohio State Univ., Wooster (USA). Agronomy Dept. Ohio Agricultural Research and Development Center) ; Wernsman, E.A ; Fitzmurice, W.P ; Burns, J.A</creatorcontrib><description>The tobacco (Nicotiana tabacum L.) breeding line NC 152 is a doubled haploid that possesses an addition chromosome from Nicotiana africana (Merxm. and Buttler). A gene on this chromosome confers potyvirus resistance (PotyR). Our objective was to use the addition chromosome as a base on which to construct a designer chromosome containing a foreign gene linkage package. A mutant dhfr gene conferring resistance to methotrexate (Mtx) was inserted into NC 152-haploid (n=25) leaf tissue via Agrobacterium tumefaciens-mediated transformation. After chromosome doubling, 135 NC 152dhfr transformants (2n=50) were pollinated with the potyvirus-susceptible (PotyS) cultivar 'McNair 944' (2n=48). Linkage analysis was performed in the BC1 generation. Two transformants, NC 152dhfr-996 and NC 152dhfr-1517, exhibited complete linkage between Mtx resistance (MtxR) and PotyR. Segregants from these two transformants which were MtxR and PotyR possessed 49 chromosomes, while Mtx sensitive (MtxS) and PotyS progeny possessed 48 chromosomes. Eighty percent of the NC 152dhfr transformants transmitted the dhfr gene as one locus. Other foreign genes can be directed to the addition chromosome through transformation followed by selection for single loci with linkage to PotyR or MtxR. The integrity of both the foreign-gene linkage package and the rest of the genome will be maintained because recombination between the N. africiana and the N. tabacum chromosomes has not been observed.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/BF00221136</identifier><identifier>PMID: 24190470</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>AGROBACTERIUM TUMEFACIENS ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genetic engineering ; Genetic technics ; Linkage analysis ; MANIPULACION DE CROMOSOMAS ; MANIPULATION CHROMOSOMIQUE ; Methods. Procedures. Technologies ; Methotrexate resistance ; Nicotiana africana ; NICOTIANA TABACUM ; POTYVIRUS ; Potyvirus resistance ; RESISTANCE AUX MALADIES ; RESISTANCE AUX PRODUITS CHIMIQUES ; RESISTENCIA A LA ENFERMEDAD ; RESISTENCIA QUIMICA ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>Theoretical and applied genetics, 1994-02, Vol.87 (7), p.837-842</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-46b1d84bea2146d72ebaef48c4c5bf687df96a39a85e6b68ee7a87d5eab6de9a3</citedby><cites>FETCH-LOGICAL-c369t-46b1d84bea2146d72ebaef48c4c5bf687df96a39a85e6b68ee7a87d5eab6de9a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3936607$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24190470$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Campbell, K.G. (Ohio State Univ., Wooster (USA). Agronomy Dept. Ohio Agricultural Research and Development Center)</creatorcontrib><creatorcontrib>Wernsman, E.A</creatorcontrib><creatorcontrib>Fitzmurice, W.P</creatorcontrib><creatorcontrib>Burns, J.A</creatorcontrib><title>Construction of a designer chromosome in tobacco</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>The tobacco (Nicotiana tabacum L.) breeding line NC 152 is a doubled haploid that possesses an addition chromosome from Nicotiana africana (Merxm. and Buttler). A gene on this chromosome confers potyvirus resistance (PotyR). Our objective was to use the addition chromosome as a base on which to construct a designer chromosome containing a foreign gene linkage package. A mutant dhfr gene conferring resistance to methotrexate (Mtx) was inserted into NC 152-haploid (n=25) leaf tissue via Agrobacterium tumefaciens-mediated transformation. After chromosome doubling, 135 NC 152dhfr transformants (2n=50) were pollinated with the potyvirus-susceptible (PotyS) cultivar 'McNair 944' (2n=48). Linkage analysis was performed in the BC1 generation. Two transformants, NC 152dhfr-996 and NC 152dhfr-1517, exhibited complete linkage between Mtx resistance (MtxR) and PotyR. Segregants from these two transformants which were MtxR and PotyR possessed 49 chromosomes, while Mtx sensitive (MtxS) and PotyS progeny possessed 48 chromosomes. Eighty percent of the NC 152dhfr transformants transmitted the dhfr gene as one locus. Other foreign genes can be directed to the addition chromosome through transformation followed by selection for single loci with linkage to PotyR or MtxR. The integrity of both the foreign-gene linkage package and the rest of the genome will be maintained because recombination between the N. africiana and the N. tabacum chromosomes has not been observed.</description><subject>AGROBACTERIUM TUMEFACIENS</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Linkage analysis</subject><subject>MANIPULACION DE CROMOSOMAS</subject><subject>MANIPULATION CHROMOSOMIQUE</subject><subject>Methods. Procedures. Technologies</subject><subject>Methotrexate resistance</subject><subject>Nicotiana africana</subject><subject>NICOTIANA TABACUM</subject><subject>POTYVIRUS</subject><subject>Potyvirus resistance</subject><subject>RESISTANCE AUX MALADIES</subject><subject>RESISTANCE AUX PRODUITS CHIMIQUES</subject><subject>RESISTENCIA A LA ENFERMEDAD</subject><subject>RESISTENCIA QUIMICA</subject><subject>TRANSFERENCIA DE GENES</subject><subject>TRANSFERT DE GENE</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNpF0D1PwzAQh3ELgWgpLIwMKAMDQgqcX-LEIy3lRarEAnN0cS4lKImLnQx8e4JaynTS6af_8DB2zuGWA6R380cAITiX-oBNuZIiFkKJQzYFUBAnaSIm7CSETxhZAvKYTYTiBlQKUwYL14XeD7avXRe5KsKopFCvO_KR_fCudcG1FNVd1LsCrXWn7KjCJtDZ7s7Y--PybfEcr16fXhb3q9hKbfpY6YKXmSoIBVe6TAUVSJXKrLJJUeksLSujURrMEtKFzohSHJ8JYaFLMihn7Hq7u_Hua6DQ520dLDUNduSGkHOljEg1cD3Smy213oXgqco3vm7Rf-cc8t9C-X-hEV_udoeipXJP_5KM4GoHMFhsKo-drcPeSSO1hnRkF1tWoctx7UfysDRqzsEY-QP7bnSJ</recordid><startdate>19940201</startdate><enddate>19940201</enddate><creator>Campbell, K.G. (Ohio State Univ., Wooster (USA). Agronomy Dept. Ohio Agricultural Research and Development Center)</creator><creator>Wernsman, E.A</creator><creator>Fitzmurice, W.P</creator><creator>Burns, J.A</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940201</creationdate><title>Construction of a designer chromosome in tobacco</title><author>Campbell, K.G. (Ohio State Univ., Wooster (USA). Agronomy Dept. Ohio Agricultural Research and Development Center) ; Wernsman, E.A ; Fitzmurice, W.P ; Burns, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-46b1d84bea2146d72ebaef48c4c5bf687df96a39a85e6b68ee7a87d5eab6de9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>AGROBACTERIUM TUMEFACIENS</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Linkage analysis</topic><topic>MANIPULACION DE CROMOSOMAS</topic><topic>MANIPULATION CHROMOSOMIQUE</topic><topic>Methods. Procedures. Technologies</topic><topic>Methotrexate resistance</topic><topic>Nicotiana africana</topic><topic>NICOTIANA TABACUM</topic><topic>POTYVIRUS</topic><topic>Potyvirus resistance</topic><topic>RESISTANCE AUX MALADIES</topic><topic>RESISTANCE AUX PRODUITS CHIMIQUES</topic><topic>RESISTENCIA A LA ENFERMEDAD</topic><topic>RESISTENCIA QUIMICA</topic><topic>TRANSFERENCIA DE GENES</topic><topic>TRANSFERT DE GENE</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Campbell, K.G. (Ohio State Univ., Wooster (USA). Agronomy Dept. Ohio Agricultural Research and Development Center)</creatorcontrib><creatorcontrib>Wernsman, E.A</creatorcontrib><creatorcontrib>Fitzmurice, W.P</creatorcontrib><creatorcontrib>Burns, J.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Campbell, K.G. (Ohio State Univ., Wooster (USA). Agronomy Dept. Ohio Agricultural Research and Development Center)</au><au>Wernsman, E.A</au><au>Fitzmurice, W.P</au><au>Burns, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of a designer chromosome in tobacco</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>1994-02-01</date><risdate>1994</risdate><volume>87</volume><issue>7</issue><spage>837</spage><epage>842</epage><pages>837-842</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>The tobacco (Nicotiana tabacum L.) breeding line NC 152 is a doubled haploid that possesses an addition chromosome from Nicotiana africana (Merxm. and Buttler). A gene on this chromosome confers potyvirus resistance (PotyR). Our objective was to use the addition chromosome as a base on which to construct a designer chromosome containing a foreign gene linkage package. A mutant dhfr gene conferring resistance to methotrexate (Mtx) was inserted into NC 152-haploid (n=25) leaf tissue via Agrobacterium tumefaciens-mediated transformation. After chromosome doubling, 135 NC 152dhfr transformants (2n=50) were pollinated with the potyvirus-susceptible (PotyS) cultivar 'McNair 944' (2n=48). Linkage analysis was performed in the BC1 generation. Two transformants, NC 152dhfr-996 and NC 152dhfr-1517, exhibited complete linkage between Mtx resistance (MtxR) and PotyR. Segregants from these two transformants which were MtxR and PotyR possessed 49 chromosomes, while Mtx sensitive (MtxS) and PotyS progeny possessed 48 chromosomes. Eighty percent of the NC 152dhfr transformants transmitted the dhfr gene as one locus. Other foreign genes can be directed to the addition chromosome through transformation followed by selection for single loci with linkage to PotyR or MtxR. The integrity of both the foreign-gene linkage package and the rest of the genome will be maintained because recombination between the N. africiana and the N. tabacum chromosomes has not been observed.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>24190470</pmid><doi>10.1007/BF00221136</doi><tpages>6</tpages></addata></record> |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | AGROBACTERIUM TUMEFACIENS Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology GENE GENES Genetic engineering Genetic technics Linkage analysis MANIPULACION DE CROMOSOMAS MANIPULATION CHROMOSOMIQUE Methods. Procedures. Technologies Methotrexate resistance Nicotiana africana NICOTIANA TABACUM POTYVIRUS Potyvirus resistance RESISTANCE AUX MALADIES RESISTANCE AUX PRODUITS CHIMIQUES RESISTENCIA A LA ENFERMEDAD RESISTENCIA QUIMICA TRANSFERENCIA DE GENES TRANSFERT DE GENE Transgenic animals and transgenic plants Transgenic plants |
| title | Construction of a designer chromosome in tobacco |
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