Differential seminal plasma proteome according to semen retrieval in men with spinal cord injury
Objective To evaluate protein expression profile and to quantify proteins present in seminal plasma from men with spinal cord injury (SCI) and healthy men without SCI. Design Experimental study. Setting University hospital. Patient(s) Twelve SCI patients divided into two groups, six who underwent el...
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| Veröffentlicht in: | Fertility and sterility 2013-10, Vol.100 (4), p.959-969.e3 |
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| creator | da Silva, Barbara Ferreira, M.Sc Souza, Gustavo Henrique Martins Ferreira, Ph.D Turco, Edson Guimarães lo, D.V.M., Ph.D Del Giudice, Paula Toni, M.Sc Soler, Thiesa Butterby, D.V.M., Ph.D Spaine, Deborah Montagnini, Ph.D Borrelli, Milton, M.D Gozzo, Fábio César, Ph.D Pilau, Eduardo Jorge, Ph.D Garcia, Jerusa Simone, Ph.D Ferreira, Christina Ramires, D.V.M., Ph.D Eberlin, Marcos Nogueira, Ph.D Bertolla, Ricardo Pimenta, D.V.M., Ph.D |
| description | Objective To evaluate protein expression profile and to quantify proteins present in seminal plasma from men with spinal cord injury (SCI) and healthy men without SCI. Design Experimental study. Setting University hospital. Patient(s) Twelve SCI patients divided into two groups, six who underwent electroejaculation (EEJ) and six who underwent penile vibratory stimulation (PVS); and ten control subjects presenting normal sperm motility and concentration. Intervention(s) EEJ and PVS. Main Outcome Measure(s) The seminal plasma protein profile was analyzed by two proteomic strategies: data-independent label-free quantitative proteomics (MSE ) and two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (2D SDS-PAGE). Result(s) A total of 638 different proteins were identified by MSE and 18 by 2D SDS-PAGE followed by tandem mass spectrometry. Interactome analysis showed key reproductive biologic processes—insemination, sperm and oocyte fusion, and acrosome reaction—related to all groups, as were triglyceride stimuli. Processes related to actin and muscle function and to iron oxidation, transportation, and homeostasis were found only in the EEJ and PVS groups; response to hydrogen peroxide and increased immune response was found only in the PVS group. Conclusion(s) This study was able to demonstrate differential protein expression among control, PVS, and EEJ groups; SCI is responsible for alterations in seminal plasma protein profile leading to a deviation from homeostasis; proteins reported in both PVS and EEJ groups correlate with the pathophysiology of SCI-related infertility. |
| doi_str_mv | 10.1016/j.fertnstert.2013.06.009 |
| format | Article |
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Design Experimental study. Setting University hospital. Patient(s) Twelve SCI patients divided into two groups, six who underwent electroejaculation (EEJ) and six who underwent penile vibratory stimulation (PVS); and ten control subjects presenting normal sperm motility and concentration. Intervention(s) EEJ and PVS. Main Outcome Measure(s) The seminal plasma protein profile was analyzed by two proteomic strategies: data-independent label-free quantitative proteomics (MSE ) and two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (2D SDS-PAGE). Result(s) A total of 638 different proteins were identified by MSE and 18 by 2D SDS-PAGE followed by tandem mass spectrometry. Interactome analysis showed key reproductive biologic processes—insemination, sperm and oocyte fusion, and acrosome reaction—related to all groups, as were triglyceride stimuli. Processes related to actin and muscle function and to iron oxidation, transportation, and homeostasis were found only in the EEJ and PVS groups; response to hydrogen peroxide and increased immune response was found only in the PVS group. Conclusion(s) This study was able to demonstrate differential protein expression among control, PVS, and EEJ groups; SCI is responsible for alterations in seminal plasma protein profile leading to a deviation from homeostasis; proteins reported in both PVS and EEJ groups correlate with the pathophysiology of SCI-related infertility.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2013.06.009</identifier><identifier>PMID: 23849843</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>acrosome ; actin ; Adult ; Biomarkers ; Biomarkers - analysis ; Case-Control Studies ; Electrophoresis, Gel, Two-Dimensional ; Electrophoresis, Polyacrylamide Gel ; functional enrichment ; homeostasis ; Hospitals, University ; Humans ; hydrogen peroxide ; immune response ; Internal Medicine ; Male ; men ; muscles ; Obstetrics and Gynecology ; oocytes ; oxidation ; pathophysiology ; patients ; polyacrylamide gel electrophoresis ; protein synthesis ; Proteins - analysis ; proteome ; proteomics ; Proteomics - methods ; Semen - chemistry ; Semen Analysis - methods ; seminal plasma ; seminal plasma proteins ; Spectrometry, Mass, Electrospray Ionization ; Sperm Count ; Sperm Motility ; Sperm Retrieval ; spinal cord ; Spinal Cord Injuries - metabolism ; Spinal Cord Injuries - physiopathology ; spinal cord injury ; Tandem Mass Spectrometry ; transportation ; triacylglycerols ; Young Adult</subject><ispartof>Fertility and sterility, 2013-10, Vol.100 (4), p.959-969.e3</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2013 American Society for Reproductive Medicine</rights><rights>Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-2b11a21ff918822129b476e3d65e829508bc469cc0fd9a7dd711bc9c55a1df9e3</citedby><cites>FETCH-LOGICAL-c503t-2b11a21ff918822129b476e3d65e829508bc469cc0fd9a7dd711bc9c55a1df9e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028213007036$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23849843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva, Barbara Ferreira, M.Sc</creatorcontrib><creatorcontrib>Souza, Gustavo Henrique Martins Ferreira, Ph.D</creatorcontrib><creatorcontrib>Turco, Edson Guimarães lo, D.V.M., Ph.D</creatorcontrib><creatorcontrib>Del Giudice, Paula Toni, M.Sc</creatorcontrib><creatorcontrib>Soler, Thiesa Butterby, D.V.M., Ph.D</creatorcontrib><creatorcontrib>Spaine, Deborah Montagnini, Ph.D</creatorcontrib><creatorcontrib>Borrelli, Milton, M.D</creatorcontrib><creatorcontrib>Gozzo, Fábio César, Ph.D</creatorcontrib><creatorcontrib>Pilau, Eduardo Jorge, Ph.D</creatorcontrib><creatorcontrib>Garcia, Jerusa Simone, Ph.D</creatorcontrib><creatorcontrib>Ferreira, Christina Ramires, D.V.M., Ph.D</creatorcontrib><creatorcontrib>Eberlin, Marcos Nogueira, Ph.D</creatorcontrib><creatorcontrib>Bertolla, Ricardo Pimenta, D.V.M., Ph.D</creatorcontrib><title>Differential seminal plasma proteome according to semen retrieval in men with spinal cord injury</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To evaluate protein expression profile and to quantify proteins present in seminal plasma from men with spinal cord injury (SCI) and healthy men without SCI. Design Experimental study. Setting University hospital. Patient(s) Twelve SCI patients divided into two groups, six who underwent electroejaculation (EEJ) and six who underwent penile vibratory stimulation (PVS); and ten control subjects presenting normal sperm motility and concentration. Intervention(s) EEJ and PVS. Main Outcome Measure(s) The seminal plasma protein profile was analyzed by two proteomic strategies: data-independent label-free quantitative proteomics (MSE ) and two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (2D SDS-PAGE). Result(s) A total of 638 different proteins were identified by MSE and 18 by 2D SDS-PAGE followed by tandem mass spectrometry. Interactome analysis showed key reproductive biologic processes—insemination, sperm and oocyte fusion, and acrosome reaction—related to all groups, as were triglyceride stimuli. Processes related to actin and muscle function and to iron oxidation, transportation, and homeostasis were found only in the EEJ and PVS groups; response to hydrogen peroxide and increased immune response was found only in the PVS group. Conclusion(s) This study was able to demonstrate differential protein expression among control, PVS, and EEJ groups; SCI is responsible for alterations in seminal plasma protein profile leading to a deviation from homeostasis; proteins reported in both PVS and EEJ groups correlate with the pathophysiology of SCI-related infertility.</description><subject>acrosome</subject><subject>actin</subject><subject>Adult</subject><subject>Biomarkers</subject><subject>Biomarkers - analysis</subject><subject>Case-Control Studies</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>functional enrichment</subject><subject>homeostasis</subject><subject>Hospitals, University</subject><subject>Humans</subject><subject>hydrogen peroxide</subject><subject>immune response</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>men</subject><subject>muscles</subject><subject>Obstetrics and Gynecology</subject><subject>oocytes</subject><subject>oxidation</subject><subject>pathophysiology</subject><subject>patients</subject><subject>polyacrylamide gel electrophoresis</subject><subject>protein synthesis</subject><subject>Proteins - analysis</subject><subject>proteome</subject><subject>proteomics</subject><subject>Proteomics - methods</subject><subject>Semen - chemistry</subject><subject>Semen Analysis - methods</subject><subject>seminal plasma</subject><subject>seminal plasma proteins</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Sperm Count</subject><subject>Sperm Motility</subject><subject>Sperm Retrieval</subject><subject>spinal cord</subject><subject>Spinal Cord Injuries - metabolism</subject><subject>Spinal Cord Injuries - physiopathology</subject><subject>spinal cord injury</subject><subject>Tandem Mass Spectrometry</subject><subject>transportation</subject><subject>triacylglycerols</subject><subject>Young Adult</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhi0EotuFV4AcuSSM7diJL0hQoCBV4lB6No4zKQ6Js9hO0b49DltA4sTFI42--Wf8zxBSUKgoUPlyrAYMyceU34oB5RXICkA9IDsqhCyFFPwh2QFQUQJr2Rk5j3EEAEkb9picMd7Wqq35jnx564ashT45MxURZ-dzPEwmzqY4hCXhMmNhrF1C7_xtkZYNQl8ETMHhXYadL7bED5e-FvHwq36jc35cw_EJeTSYKeLT-7gnN-_ffb74UF59uvx48fqqtAJ4KllHqWF0GBRtW8YoU13dSOS9FNgyJaDtbC2VtTD0yjR931DaWWWFMLQfFPI9eXHSzUN_XzEmPbtocZqMx2WNmta1Yk3Ncrc9aU-oDUuMAQd9CG424agp6M1fPeq__urNXw1SZ39z6bP7Lms3Y_-n8LehGXh-AgazaHMbXNQ311lB5F0wRes2E29OBGY37hwGHa1Db7F3AW3S_eL-Z45X_4jYyXlnzfQNjxjHZQ15D_nXOjIN-nq7hO0QKAdogEv-ExoqsMg</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>da Silva, Barbara Ferreira, M.Sc</creator><creator>Souza, Gustavo Henrique Martins Ferreira, Ph.D</creator><creator>Turco, Edson Guimarães lo, D.V.M., Ph.D</creator><creator>Del Giudice, Paula Toni, M.Sc</creator><creator>Soler, Thiesa Butterby, D.V.M., Ph.D</creator><creator>Spaine, Deborah Montagnini, Ph.D</creator><creator>Borrelli, Milton, M.D</creator><creator>Gozzo, Fábio César, Ph.D</creator><creator>Pilau, Eduardo Jorge, Ph.D</creator><creator>Garcia, Jerusa Simone, Ph.D</creator><creator>Ferreira, Christina Ramires, D.V.M., Ph.D</creator><creator>Eberlin, Marcos Nogueira, Ph.D</creator><creator>Bertolla, Ricardo Pimenta, D.V.M., Ph.D</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131001</creationdate><title>Differential seminal plasma proteome according to semen retrieval in men with spinal cord injury</title><author>da Silva, Barbara Ferreira, M.Sc ; Souza, Gustavo Henrique Martins Ferreira, Ph.D ; Turco, Edson Guimarães lo, D.V.M., Ph.D ; Del Giudice, Paula Toni, M.Sc ; Soler, Thiesa Butterby, D.V.M., Ph.D ; Spaine, Deborah Montagnini, Ph.D ; Borrelli, Milton, M.D ; Gozzo, Fábio César, Ph.D ; Pilau, Eduardo Jorge, Ph.D ; Garcia, Jerusa Simone, Ph.D ; Ferreira, Christina Ramires, D.V.M., Ph.D ; Eberlin, Marcos Nogueira, Ph.D ; Bertolla, Ricardo Pimenta, D.V.M., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-2b11a21ff918822129b476e3d65e829508bc469cc0fd9a7dd711bc9c55a1df9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>acrosome</topic><topic>actin</topic><topic>Adult</topic><topic>Biomarkers</topic><topic>Biomarkers - analysis</topic><topic>Case-Control Studies</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>functional enrichment</topic><topic>homeostasis</topic><topic>Hospitals, University</topic><topic>Humans</topic><topic>hydrogen peroxide</topic><topic>immune response</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>men</topic><topic>muscles</topic><topic>Obstetrics and Gynecology</topic><topic>oocytes</topic><topic>oxidation</topic><topic>pathophysiology</topic><topic>patients</topic><topic>polyacrylamide gel electrophoresis</topic><topic>protein synthesis</topic><topic>Proteins - analysis</topic><topic>proteome</topic><topic>proteomics</topic><topic>Proteomics - methods</topic><topic>Semen - chemistry</topic><topic>Semen Analysis - methods</topic><topic>seminal plasma</topic><topic>seminal plasma proteins</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Sperm Count</topic><topic>Sperm Motility</topic><topic>Sperm Retrieval</topic><topic>spinal cord</topic><topic>Spinal Cord Injuries - metabolism</topic><topic>Spinal Cord Injuries - physiopathology</topic><topic>spinal cord injury</topic><topic>Tandem Mass Spectrometry</topic><topic>transportation</topic><topic>triacylglycerols</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Silva, Barbara Ferreira, M.Sc</creatorcontrib><creatorcontrib>Souza, Gustavo Henrique Martins Ferreira, Ph.D</creatorcontrib><creatorcontrib>Turco, Edson Guimarães lo, D.V.M., Ph.D</creatorcontrib><creatorcontrib>Del Giudice, Paula Toni, M.Sc</creatorcontrib><creatorcontrib>Soler, Thiesa Butterby, D.V.M., Ph.D</creatorcontrib><creatorcontrib>Spaine, Deborah Montagnini, Ph.D</creatorcontrib><creatorcontrib>Borrelli, Milton, M.D</creatorcontrib><creatorcontrib>Gozzo, Fábio César, Ph.D</creatorcontrib><creatorcontrib>Pilau, Eduardo Jorge, Ph.D</creatorcontrib><creatorcontrib>Garcia, Jerusa Simone, Ph.D</creatorcontrib><creatorcontrib>Ferreira, Christina Ramires, D.V.M., Ph.D</creatorcontrib><creatorcontrib>Eberlin, Marcos Nogueira, Ph.D</creatorcontrib><creatorcontrib>Bertolla, Ricardo Pimenta, D.V.M., Ph.D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Silva, Barbara Ferreira, M.Sc</au><au>Souza, Gustavo Henrique Martins Ferreira, Ph.D</au><au>Turco, Edson Guimarães lo, D.V.M., Ph.D</au><au>Del Giudice, Paula Toni, M.Sc</au><au>Soler, Thiesa Butterby, D.V.M., Ph.D</au><au>Spaine, Deborah Montagnini, Ph.D</au><au>Borrelli, Milton, M.D</au><au>Gozzo, Fábio César, Ph.D</au><au>Pilau, Eduardo Jorge, Ph.D</au><au>Garcia, Jerusa Simone, Ph.D</au><au>Ferreira, Christina Ramires, D.V.M., Ph.D</au><au>Eberlin, Marcos Nogueira, Ph.D</au><au>Bertolla, Ricardo Pimenta, D.V.M., Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential seminal plasma proteome according to semen retrieval in men with spinal cord injury</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>100</volume><issue>4</issue><spage>959</spage><epage>969.e3</epage><pages>959-969.e3</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><abstract>Objective To evaluate protein expression profile and to quantify proteins present in seminal plasma from men with spinal cord injury (SCI) and healthy men without SCI. Design Experimental study. Setting University hospital. Patient(s) Twelve SCI patients divided into two groups, six who underwent electroejaculation (EEJ) and six who underwent penile vibratory stimulation (PVS); and ten control subjects presenting normal sperm motility and concentration. Intervention(s) EEJ and PVS. Main Outcome Measure(s) The seminal plasma protein profile was analyzed by two proteomic strategies: data-independent label-free quantitative proteomics (MSE ) and two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (2D SDS-PAGE). Result(s) A total of 638 different proteins were identified by MSE and 18 by 2D SDS-PAGE followed by tandem mass spectrometry. Interactome analysis showed key reproductive biologic processes—insemination, sperm and oocyte fusion, and acrosome reaction—related to all groups, as were triglyceride stimuli. Processes related to actin and muscle function and to iron oxidation, transportation, and homeostasis were found only in the EEJ and PVS groups; response to hydrogen peroxide and increased immune response was found only in the PVS group. Conclusion(s) This study was able to demonstrate differential protein expression among control, PVS, and EEJ groups; SCI is responsible for alterations in seminal plasma protein profile leading to a deviation from homeostasis; proteins reported in both PVS and EEJ groups correlate with the pathophysiology of SCI-related infertility.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23849843</pmid><doi>10.1016/j.fertnstert.2013.06.009</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Fertility and sterility, 2013-10, Vol.100 (4), p.959-969.e3 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | acrosome actin Adult Biomarkers Biomarkers - analysis Case-Control Studies Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel functional enrichment homeostasis Hospitals, University Humans hydrogen peroxide immune response Internal Medicine Male men muscles Obstetrics and Gynecology oocytes oxidation pathophysiology patients polyacrylamide gel electrophoresis protein synthesis Proteins - analysis proteome proteomics Proteomics - methods Semen - chemistry Semen Analysis - methods seminal plasma seminal plasma proteins Spectrometry, Mass, Electrospray Ionization Sperm Count Sperm Motility Sperm Retrieval spinal cord Spinal Cord Injuries - metabolism Spinal Cord Injuries - physiopathology spinal cord injury Tandem Mass Spectrometry transportation triacylglycerols Young Adult |
| title | Differential seminal plasma proteome according to semen retrieval in men with spinal cord injury |
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