Stabilized Wittig olefination for bioconjugation
Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP...
Gespeichert in:
| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2013-12, Vol.49 (95), p.11188-11190 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 11190 |
|---|---|
| container_issue | 95 |
| container_start_page | 11188 |
| container_title | Chemical communications (Cambridge, England) |
| container_volume | 49 |
| creator | Lum, Kenneth M Xavier, Vanessa J Ong, Michelle J-H Johannes, Charles W Chan, Kok-Ping |
| description | Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12. |
| doi_str_mv | 10.1039/c3cc45961f |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1449273467</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1449273467</sourcerecordid><originalsourceid>FETCH-LOGICAL-c287t-e4aa9bdd63091f0700289e11d1719a51fc79cc0a8d58487046fc62aefa9ec7863</originalsourceid><addsrcrecordid>eNpFkE1Lw0AQhhdRbK1e_AGSowjRnez3UUr9gIIHFb2FzWa3bEmydTc56K83tVXnMsPLwwvzIHQO-BowUTeGGEOZ4uAO0BQIpzmj8v1wezOVC0LZBJ2ktMbjAJPHaFJQYFiAnCL83OvKN_7L1tmb73u_ykJjne9070OXuRCzygcTuvWw-olO0ZHTTbJn-z1Dr3eLl_lDvny6f5zfLnNTSNHnlmqtqrrmBCtwWGBcSGUBahCgNANnhDIGa1kzSaXAlDvDC22dVtYIyckMXe56NzF8DDb1ZeuTsU2jOxuGVAKlqhh_42JEr3aoiSGlaF25ib7V8bMEXG4Nlf-GRvhi3ztUra3_0F8l5BupUGDV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449273467</pqid></control><display><type>article</type><title>Stabilized Wittig olefination for bioconjugation</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Lum, Kenneth M ; Xavier, Vanessa J ; Ong, Michelle J-H ; Johannes, Charles W ; Chan, Kok-Ping</creator><creatorcontrib>Lum, Kenneth M ; Xavier, Vanessa J ; Ong, Michelle J-H ; Johannes, Charles W ; Chan, Kok-Ping</creatorcontrib><description>Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c3cc45961f</identifier><identifier>PMID: 24150718</identifier><language>eng</language><publisher>England</publisher><subject>Aldehydes - chemistry ; Alkenes - chemistry ; Fluorescent Dyes - chemistry ; HeLa Cells ; Humans ; Microscopy, Fluorescence ; Phosphorus - chemistry ; Tacrolimus Binding Protein 1A - chemistry ; Tacrolimus Binding Protein 1A - metabolism</subject><ispartof>Chemical communications (Cambridge, England), 2013-12, Vol.49 (95), p.11188-11190</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c287t-e4aa9bdd63091f0700289e11d1719a51fc79cc0a8d58487046fc62aefa9ec7863</citedby><cites>FETCH-LOGICAL-c287t-e4aa9bdd63091f0700289e11d1719a51fc79cc0a8d58487046fc62aefa9ec7863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24150718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lum, Kenneth M</creatorcontrib><creatorcontrib>Xavier, Vanessa J</creatorcontrib><creatorcontrib>Ong, Michelle J-H</creatorcontrib><creatorcontrib>Johannes, Charles W</creatorcontrib><creatorcontrib>Chan, Kok-Ping</creatorcontrib><title>Stabilized Wittig olefination for bioconjugation</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.</description><subject>Aldehydes - chemistry</subject><subject>Alkenes - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Microscopy, Fluorescence</subject><subject>Phosphorus - chemistry</subject><subject>Tacrolimus Binding Protein 1A - chemistry</subject><subject>Tacrolimus Binding Protein 1A - metabolism</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1Lw0AQhhdRbK1e_AGSowjRnez3UUr9gIIHFb2FzWa3bEmydTc56K83tVXnMsPLwwvzIHQO-BowUTeGGEOZ4uAO0BQIpzmj8v1wezOVC0LZBJ2ktMbjAJPHaFJQYFiAnCL83OvKN_7L1tmb73u_ykJjne9070OXuRCzygcTuvWw-olO0ZHTTbJn-z1Dr3eLl_lDvny6f5zfLnNTSNHnlmqtqrrmBCtwWGBcSGUBahCgNANnhDIGa1kzSaXAlDvDC22dVtYIyckMXe56NzF8DDb1ZeuTsU2jOxuGVAKlqhh_42JEr3aoiSGlaF25ib7V8bMEXG4Nlf-GRvhi3ztUra3_0F8l5BupUGDV</recordid><startdate>20131211</startdate><enddate>20131211</enddate><creator>Lum, Kenneth M</creator><creator>Xavier, Vanessa J</creator><creator>Ong, Michelle J-H</creator><creator>Johannes, Charles W</creator><creator>Chan, Kok-Ping</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131211</creationdate><title>Stabilized Wittig olefination for bioconjugation</title><author>Lum, Kenneth M ; Xavier, Vanessa J ; Ong, Michelle J-H ; Johannes, Charles W ; Chan, Kok-Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c287t-e4aa9bdd63091f0700289e11d1719a51fc79cc0a8d58487046fc62aefa9ec7863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aldehydes - chemistry</topic><topic>Alkenes - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Microscopy, Fluorescence</topic><topic>Phosphorus - chemistry</topic><topic>Tacrolimus Binding Protein 1A - chemistry</topic><topic>Tacrolimus Binding Protein 1A - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lum, Kenneth M</creatorcontrib><creatorcontrib>Xavier, Vanessa J</creatorcontrib><creatorcontrib>Ong, Michelle J-H</creatorcontrib><creatorcontrib>Johannes, Charles W</creatorcontrib><creatorcontrib>Chan, Kok-Ping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lum, Kenneth M</au><au>Xavier, Vanessa J</au><au>Ong, Michelle J-H</au><au>Johannes, Charles W</au><au>Chan, Kok-Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stabilized Wittig olefination for bioconjugation</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2013-12-11</date><risdate>2013</risdate><volume>49</volume><issue>95</issue><spage>11188</spage><epage>11190</epage><pages>11188-11190</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.</abstract><cop>England</cop><pmid>24150718</pmid><doi>10.1039/c3cc45961f</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2013-12, Vol.49 (95), p.11188-11190 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1449273467 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Aldehydes - chemistry Alkenes - chemistry Fluorescent Dyes - chemistry HeLa Cells Humans Microscopy, Fluorescence Phosphorus - chemistry Tacrolimus Binding Protein 1A - chemistry Tacrolimus Binding Protein 1A - metabolism |
| title | Stabilized Wittig olefination for bioconjugation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T15%3A16%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stabilized%20Wittig%20olefination%20for%20bioconjugation&rft.jtitle=Chemical%20communications%20(Cambridge,%20England)&rft.au=Lum,%20Kenneth%20M&rft.date=2013-12-11&rft.volume=49&rft.issue=95&rft.spage=11188&rft.epage=11190&rft.pages=11188-11190&rft.issn=1359-7345&rft.eissn=1364-548X&rft_id=info:doi/10.1039/c3cc45961f&rft_dat=%3Cproquest_cross%3E1449273467%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1449273467&rft_id=info:pmid/24150718&rfr_iscdi=true |