Stabilized Wittig olefination for bioconjugation

Stabilized Wittig olefination for bioconjugation

Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2013-12, Vol.49 (95), p.11188-11190
Hauptverfasser: Lum, Kenneth M, Xavier, Vanessa J, Ong, Michelle J-H, Johannes, Charles W, Chan, Kok-Ping
Format: Artikel
Sprache:eng
Schlagworte:
Aldehydes - chemistry
Alkenes - chemistry
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Microscopy, Fluorescence
Phosphorus - chemistry
Tacrolimus Binding Protein 1A - chemistry
Tacrolimus Binding Protein 1A - metabolism
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Zusammenfassung:Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc45961f