In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant
A rapid clonal propagation system for Clerodendrum colebrookianum Walp. (Verbenaceae), a anti-hypertension folk medicinal shrub has been developed. A range of cytokinins has been investigated for multiple shoot induction with shoot apex, axillary shoot, leaf, petiole and root explants. Optimum shoot...
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| Veröffentlicht in: | Plant cell reports 1995-05, Vol.14 (8), p.493-496 |
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| container_title | Plant cell reports |
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| creator | MAO, A. A WETTEN, A FAY, M CALIGARI, P. D. S |
| description | A rapid clonal propagation system for Clerodendrum colebrookianum Walp. (Verbenaceae), a anti-hypertension folk medicinal shrub has been developed. A range of cytokinins has been investigated for multiple shoot induction with shoot apex, axillary shoot, leaf, petiole and root explants. Optimum shoot induction occurred with axillary buds using 6-benzyladenine where an average of 21 shoots were produced per explant in 6 weeks. Subculturing the newly produced shoots, by separating into groups of five shoots, produced an average of 43 new shoots per culture within 4 weeks. In vitro rooting and weaning of over 200 plantlets was completely successful. Cytological studies revealed no visible abnormalities in chromosome number. |
| doi_str_mv | 10.1007/BF00232781 |
| format | Article |
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A ; WETTEN, A ; FAY, M ; CALIGARI, P. D. S</creator><creatorcontrib>MAO, A. A ; WETTEN, A ; FAY, M ; CALIGARI, P. D. S</creatorcontrib><description>A rapid clonal propagation system for Clerodendrum colebrookianum Walp. (Verbenaceae), a anti-hypertension folk medicinal shrub has been developed. A range of cytokinins has been investigated for multiple shoot induction with shoot apex, axillary shoot, leaf, petiole and root explants. Optimum shoot induction occurred with axillary buds using 6-benzyladenine where an average of 21 shoots were produced per explant in 6 weeks. Subculturing the newly produced shoots, by separating into groups of five shoots, produced an average of 43 new shoots per culture within 4 weeks. In vitro rooting and weaning of over 200 plantlets was completely successful. 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In vitro rooting and weaning of over 200 plantlets was completely successful. Cytological studies revealed no visible abnormalities in chromosome number.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In vitro propagation: entire plant regeneration from tissues and cell cultures</subject><subject>Methods. Procedures. 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Technologies</topic><topic>Plant cells and fungal cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MAO, A. A</creatorcontrib><creatorcontrib>WETTEN, A</creatorcontrib><creatorcontrib>FAY, M</creatorcontrib><creatorcontrib>CALIGARI, P. D. S</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MAO, A. A</au><au>WETTEN, A</au><au>FAY, M</au><au>CALIGARI, P. D. 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| source | Springer Journals |
| subjects | Biological and medical sciences Biotechnology Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology In vitro propagation: entire plant regeneration from tissues and cell cultures Methods. Procedures. Technologies Plant cells and fungal cells |
| title | In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant |
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