Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.)

Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.)

Somatic embryo formation occurred from immature zygotic embryos within ovules of stenospermocarpic seedless grapes (Vitis vinifera L.), when cultured for two months on liquid Emershad/Ramming medium. Somatic embryos continued to proliferate after excision and transfer to Emershad/Ramming medium supp...

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Veröffentlicht in:Plant cell reports 1994-11, Vol.14 (1), p.6-12
Hauptverfasser: EMERSHAD, R. L, RAMMING, D. W
Format: Artikel
Sprache:eng
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Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
In vitro embryo culture
Methods. Procedures. Technologies
Plant cells and fungal cells
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RAMMING, D. W
description Somatic embryo formation occurred from immature zygotic embryos within ovules of stenospermocarpic seedless grapes (Vitis vinifera L.), when cultured for two months on liquid Emershad/Ramming medium. Somatic embryos continued to proliferate after excision and transfer to Emershad/Ramming medium supplemented with 1 μM benzylaminopurine and 0.65% TC agar. Plant development from somatic embryos was influenced by genotype, medium, phase (liquid, agar), stage (torpedo, mature) and their interactions. Optimal plant development occurred on Woody Plant Medium supplemented with 1.5% sucrose + 1 μM benzylaminopurine + 0.3% activated charcoal and 0.65% TC agar.
doi_str_mv 10.1007/BF00233289
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subjects Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
In vitro embryo culture
Methods. Procedures. Technologies
Plant cells and fungal cells
title Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.)
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