Growth-Dependent Expression of Multiple Species of DNA Methyltransferase in Murine Erythroleukemia Cells
Friend murine erythroleukemia cells were found to contain three distinct species of DNA (cytosine-5-)-methyltransferase (DNA MeTase) whose relative proportions were a characteristic function of the proliferative state of the cells. Rapidly proliferating cells contained a Mr190,000 species of DNA MeT...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-05, Vol.82 (9), p.2674-2678 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Bestor, Timothy H. Ingram, Vernon M. |
| description | Friend murine erythroleukemia cells were found to contain three distinct species of DNA (cytosine-5-)-methyltransferase (DNA MeTase) whose relative proportions were a characteristic function of the proliferative state of the cells. Rapidly proliferating cells contained a Mr190,000 species of DNA MeTase (DNA MeTase III), whereas cells in the late logarithmic/early plateau phase of cellular growth contained two species of Mr150,000 and 175,000 (DNA MeTases I and II); stationary phase cells contained primarily DNA MeTase I. The three species of DNA MeTase displayed structural similarities, as determined by analysis of partial proteolysis products, and have similar de novo sequence specificities in transmethylation reactions involving purified enzyme and prokaryotic DNA. The different relative proportions of the enzymes in cells under different growth conditions suggest that the three species of DNA MeTase fulfill different roles in processes leading to the perpetuation of DNA methylation patterns. |
| doi_str_mv | 10.1073/pnas.82.9.2674 |
| format | Article |
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Rapidly proliferating cells contained a Mr190,000 species of DNA MeTase (DNA MeTase III), whereas cells in the late logarithmic/early plateau phase of cellular growth contained two species of Mr150,000 and 175,000 (DNA MeTases I and II); stationary phase cells contained primarily DNA MeTase I. The three species of DNA MeTase displayed structural similarities, as determined by analysis of partial proteolysis products, and have similar de novo sequence specificities in transmethylation reactions involving purified enzyme and prokaryotic DNA. The different relative proportions of the enzymes in cells under different growth conditions suggest that the three species of DNA MeTase fulfill different roles in processes leading to the perpetuation of DNA methylation patterns.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.9.2674</identifier><identifier>PMID: 3857609</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Biological and medical sciences ; Cell Differentiation ; Cell Division ; Cell growth ; Cell Line ; Cell nucleus ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Cellular differentiation ; Chromatography ; DNA ; DNA (Cytosine-5-)-Methyltransferases - metabolism ; DNA - metabolism ; DNA replication ; Enzymes ; Erythroid cells ; Fundamental and applied biological sciences. Psychology ; Gels ; Leukemia, Erythroblastic, Acute - enzymology ; Leukemia, Erythroblastic, Acute - metabolism ; Leukemia, Erythroblastic, Acute - pathology ; Methylation ; Methyltransferases - metabolism ; Mice ; Molecular and cellular biology ; Molecular Weight</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-05, Vol.82 (9), p.2674-2678</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-87d6f4daacaa80c41524ac4970f171d41cd3e10efabcaa8424aee36eb55e0e153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25239$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25239$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9144787$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3857609$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bestor, Timothy H.</creatorcontrib><creatorcontrib>Ingram, Vernon M.</creatorcontrib><title>Growth-Dependent Expression of Multiple Species of DNA Methyltransferase in Murine Erythroleukemia Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Friend murine erythroleukemia cells were found to contain three distinct species of DNA (cytosine-5-)-methyltransferase (DNA MeTase) whose relative proportions were a characteristic function of the proliferative state of the cells. Rapidly proliferating cells contained a Mr190,000 species of DNA MeTase (DNA MeTase III), whereas cells in the late logarithmic/early plateau phase of cellular growth contained two species of Mr150,000 and 175,000 (DNA MeTases I and II); stationary phase cells contained primarily DNA MeTase I. The three species of DNA MeTase displayed structural similarities, as determined by analysis of partial proteolysis products, and have similar de novo sequence specificities in transmethylation reactions involving purified enzyme and prokaryotic DNA. The different relative proportions of the enzymes in cells under different growth conditions suggest that the three species of DNA MeTase fulfill different roles in processes leading to the perpetuation of DNA methylation patterns.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Cell growth</subject><subject>Cell Line</subject><subject>Cell nucleus</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Cellular differentiation</subject><subject>Chromatography</subject><subject>DNA</subject><subject>DNA (Cytosine-5-)-Methyltransferases - metabolism</subject><subject>DNA - metabolism</subject><subject>DNA replication</subject><subject>Enzymes</subject><subject>Erythroid cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Leukemia, Erythroblastic, Acute - enzymology</subject><subject>Leukemia, Erythroblastic, Acute - metabolism</subject><subject>Leukemia, Erythroblastic, Acute - pathology</subject><subject>Methylation</subject><subject>Methyltransferases - metabolism</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular Weight</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbFv1DAYxS0EKkdhZUBCyoC6JdiOE8cDQ3U9ClILQ8ts-ZwvxMUXp7YDvf8eR3ecrgudLPn93vf5-SH0luCCYF5-HAcVioYWoqA1Z8_QgmBB8poJ_BwtMKY8bxhlL9GrEO4wxqJq8Ak6KZuK11gsUH_p3Z_Y5xcwwtDCELPVw-ghBOOGzHXZ9WSjGS1kNyNoA2G-u_h2nl1D7Lc2ejWEDrwKkJkhwd4MkK38NvbeWZh-wcaobAnWhtfoRadsgDf78xT9-Ly6XX7Jr75ffl2eX-W6InXMG97WHWuV0ko1WDNSUaY0Exx3hJOWEd2WQDB0aj0TKZoCKGtYVxVgIFV5ij7t5o7TegOtTpG8snL0ZqP8Vjpl5GNlML386X7LUvCa8uQ_2_u9u58gRLkxQacEagA3BTl_GyVcPAkSxhpeVTSBxQ7U3oXgoTs8hmA5dyjnDmVDpZBzh8nw_jjCAd-XlvQPe10FrWyXStAmHDCRNvOGH42Zx_9Tj9ec_U-X3WRthIeYwHc78C5E5w8kTdlE-Rd-b8ir</recordid><startdate>19850501</startdate><enddate>19850501</enddate><creator>Bestor, Timothy H.</creator><creator>Ingram, Vernon M.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850501</creationdate><title>Growth-Dependent Expression of Multiple Species of DNA Methyltransferase in Murine Erythroleukemia Cells</title><author>Bestor, Timothy H. ; Ingram, Vernon M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-87d6f4daacaa80c41524ac4970f171d41cd3e10efabcaa8424aee36eb55e0e153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Cell growth</topic><topic>Cell Line</topic><topic>Cell nucleus</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Cellular differentiation</topic><topic>Chromatography</topic><topic>DNA</topic><topic>DNA (Cytosine-5-)-Methyltransferases - metabolism</topic><topic>DNA - metabolism</topic><topic>DNA replication</topic><topic>Enzymes</topic><topic>Erythroid cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Leukemia, Erythroblastic, Acute - enzymology</topic><topic>Leukemia, Erythroblastic, Acute - metabolism</topic><topic>Leukemia, Erythroblastic, Acute - pathology</topic><topic>Methylation</topic><topic>Methyltransferases - metabolism</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular Weight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bestor, Timothy H.</creatorcontrib><creatorcontrib>Ingram, Vernon M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bestor, Timothy H.</au><au>Ingram, Vernon M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth-Dependent Expression of Multiple Species of DNA Methyltransferase in Murine Erythroleukemia Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-05-01</date><risdate>1985</risdate><volume>82</volume><issue>9</issue><spage>2674</spage><epage>2678</epage><pages>2674-2678</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Friend murine erythroleukemia cells were found to contain three distinct species of DNA (cytosine-5-)-methyltransferase (DNA MeTase) whose relative proportions were a characteristic function of the proliferative state of the cells. Rapidly proliferating cells contained a Mr190,000 species of DNA MeTase (DNA MeTase III), whereas cells in the late logarithmic/early plateau phase of cellular growth contained two species of Mr150,000 and 175,000 (DNA MeTases I and II); stationary phase cells contained primarily DNA MeTase I. The three species of DNA MeTase displayed structural similarities, as determined by analysis of partial proteolysis products, and have similar de novo sequence specificities in transmethylation reactions involving purified enzyme and prokaryotic DNA. The different relative proportions of the enzymes in cells under different growth conditions suggest that the three species of DNA MeTase fulfill different roles in processes leading to the perpetuation of DNA methylation patterns.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3857609</pmid><doi>10.1073/pnas.82.9.2674</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Cell Differentiation Cell Division Cell growth Cell Line Cell nucleus Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cellular differentiation Chromatography DNA DNA (Cytosine-5-)-Methyltransferases - metabolism DNA - metabolism DNA replication Enzymes Erythroid cells Fundamental and applied biological sciences. Psychology Gels Leukemia, Erythroblastic, Acute - enzymology Leukemia, Erythroblastic, Acute - metabolism Leukemia, Erythroblastic, Acute - pathology Methylation Methyltransferases - metabolism Mice Molecular and cellular biology Molecular Weight |
| title | Growth-Dependent Expression of Multiple Species of DNA Methyltransferase in Murine Erythroleukemia Cells |
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