Sublethal High Hydrostatic Pressure Treatment Reveals the Importance of Genes Coding Cytoskeletal Protein in Escherichia Coli Morphogenesis
We studied morphologic changes after sublethal high hydrostatic pressure treatment (HPT) of Escherichia coli K-12 strains in which genes related to the cytoskeleton, cell wall, and cell division had been deleted. Some long filamentous and swelling cells were observed in wild-type bacteria, while som...
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| Veröffentlicht in: | Current microbiology 2013-11, Vol.67 (5), p.515-521 |
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| creator | Abe, Atsumu Furukawa, Soichi Migita, Yuya Tanaka, Motoharu Ogihara, Hirokazu Morinaga, Yasushi |
| description | We studied morphologic changes after sublethal high hydrostatic pressure treatment (HPT) of Escherichia coli K-12 strains in which genes related to the cytoskeleton, cell wall, and cell division had been deleted. Some long filamentous and swelling cells were observed in wild-type bacteria, while some spherical, branched, or collapsed cells were observed in deletion mutants. In particular, ΔzapA and ΔrodZ showed distinguished morphologies. ZapA supports FtsZ, a cytoskeletal protein, forming ring with ZapB. RodZ, a cytoskeletal protein, interacts with MreB, also a cytoskeletal protein, and both factors are necessary for maintaining the rod shape of the cell. These results showed that insufficient formation of FtsZ rings induced cell elongation and that insufficient formation of MreB induced a branched and collapsed cell shape. Therefore, the correct formation of the bacteria cytoskeleton by FtsZ rings and MreB is important for keeping normal cell shape during growth after HPT, and the polymerization of cytoskeletal proteins was a critical target of sublethal HPT. These results indicate that sublethal HPT induces bacterial cell morphologic change and provide important information on the role of genes involved in morphogenesis. Therefore, sublethal HPT may be a good tool for studying the morphogenesis of bacterial cells. |
| doi_str_mv | 10.1007/s00284-013-0392-8 |
| format | Article |
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Some long filamentous and swelling cells were observed in wild-type bacteria, while some spherical, branched, or collapsed cells were observed in deletion mutants. In particular, ΔzapA and ΔrodZ showed distinguished morphologies. ZapA supports FtsZ, a cytoskeletal protein, forming ring with ZapB. RodZ, a cytoskeletal protein, interacts with MreB, also a cytoskeletal protein, and both factors are necessary for maintaining the rod shape of the cell. These results showed that insufficient formation of FtsZ rings induced cell elongation and that insufficient formation of MreB induced a branched and collapsed cell shape. Therefore, the correct formation of the bacteria cytoskeleton by FtsZ rings and MreB is important for keeping normal cell shape during growth after HPT, and the polymerization of cytoskeletal proteins was a critical target of sublethal HPT. These results indicate that sublethal HPT induces bacterial cell morphologic change and provide important information on the role of genes involved in morphogenesis. Therefore, sublethal HPT may be a good tool for studying the morphogenesis of bacterial cells.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-013-0392-8</identifier><identifier>PMID: 23708427</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>Bacterial proteins ; Biomedical and Life Sciences ; Biotechnology ; Cell division ; cell growth ; cell walls ; cytoskeletal proteins ; Cytoskeletal Proteins - genetics ; Cytoskeletal Proteins - metabolism ; Cytoskeleton ; E coli ; Escherichia coli ; Escherichia coli - cytology ; Escherichia coli - physiology ; Fluid dynamics ; genes ; Hydrostatic Pressure ; Life Sciences ; Microbiology ; morphogenesis ; Morphology ; mutants ; Mutation ; pressure treatment</subject><ispartof>Current microbiology, 2013-11, Vol.67 (5), p.515-521</ispartof><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-2c6707cb653dca6370d6e8afc00ed5e240a7277001e67e6aa60ba7cbbbe1b3763</citedby><cites>FETCH-LOGICAL-c495t-2c6707cb653dca6370d6e8afc00ed5e240a7277001e67e6aa60ba7cbbbe1b3763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-013-0392-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-013-0392-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23708427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abe, Atsumu</creatorcontrib><creatorcontrib>Furukawa, Soichi</creatorcontrib><creatorcontrib>Migita, Yuya</creatorcontrib><creatorcontrib>Tanaka, Motoharu</creatorcontrib><creatorcontrib>Ogihara, Hirokazu</creatorcontrib><creatorcontrib>Morinaga, Yasushi</creatorcontrib><title>Sublethal High Hydrostatic Pressure Treatment Reveals the Importance of Genes Coding Cytoskeletal Protein in Escherichia Coli Morphogenesis</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>We studied morphologic changes after sublethal high hydrostatic pressure treatment (HPT) of Escherichia coli K-12 strains in which genes related to the cytoskeleton, cell wall, and cell division had been deleted. Some long filamentous and swelling cells were observed in wild-type bacteria, while some spherical, branched, or collapsed cells were observed in deletion mutants. In particular, ΔzapA and ΔrodZ showed distinguished morphologies. ZapA supports FtsZ, a cytoskeletal protein, forming ring with ZapB. RodZ, a cytoskeletal protein, interacts with MreB, also a cytoskeletal protein, and both factors are necessary for maintaining the rod shape of the cell. These results showed that insufficient formation of FtsZ rings induced cell elongation and that insufficient formation of MreB induced a branched and collapsed cell shape. Therefore, the correct formation of the bacteria cytoskeleton by FtsZ rings and MreB is important for keeping normal cell shape during growth after HPT, and the polymerization of cytoskeletal proteins was a critical target of sublethal HPT. These results indicate that sublethal HPT induces bacterial cell morphologic change and provide important information on the role of genes involved in morphogenesis. Therefore, sublethal HPT may be a good tool for studying the morphogenesis of bacterial cells.</description><subject>Bacterial proteins</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell division</subject><subject>cell growth</subject><subject>cell walls</subject><subject>cytoskeletal proteins</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Cytoskeleton</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - cytology</subject><subject>Escherichia coli - physiology</subject><subject>Fluid dynamics</subject><subject>genes</subject><subject>Hydrostatic Pressure</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>morphogenesis</subject><subject>Morphology</subject><subject>mutants</subject><subject>Mutation</subject><subject>pressure treatment</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkcFu1DAURSMEokPhA9iAJTbdBJ7txE6WaFQ6lYqoaLu2HOclcUniwXaQ5hv46XqUghALhGTJC593rPtulr2m8J4CyA8BgFVFDpTnwGuWV0-yDS04y6Gu6dNsA7zgeSVKepK9COEegLIa6PPshHEJVcHkJvt5szQjxkGPZGf7gewOrXch6mgNufYYwuKR3HrUccI5kq_4A_UYSByQXE5756OeDRLXkQucMZCta-3ck-0huvANkzh5r72LaGeSznkwA3prBqsTOlry2fn94PrjrA0vs2ddkuOrx_s0u_t0frvd5VdfLi63H69yU9RlzJkREqRpRMlbo0WK0gqsdGcAsC2RFaAlkzKlRSFRaC2g0YlvGqQNl4KfZmerd-_d9wVDVJMNBsdRz-iWoGhRVIxxXvL_QTmv64pWCX33F3rvFj-nIIlK7QhaUpooulImrTl47NTe20n7g6KgjqWqtVSVSlXHUtXR_ObRvDQTtr8nfrWYALYCIT3NPfo_vv6H9e061GmndO9tUHc3DGgBACXUQvIHDaG23Q</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Abe, Atsumu</creator><creator>Furukawa, Soichi</creator><creator>Migita, Yuya</creator><creator>Tanaka, Motoharu</creator><creator>Ogihara, Hirokazu</creator><creator>Morinaga, Yasushi</creator><general>Springer-Verlag</general><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20131101</creationdate><title>Sublethal High Hydrostatic Pressure Treatment Reveals the Importance of Genes Coding Cytoskeletal Protein in Escherichia Coli Morphogenesis</title><author>Abe, Atsumu ; Furukawa, Soichi ; Migita, Yuya ; Tanaka, Motoharu ; Ogihara, Hirokazu ; Morinaga, Yasushi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-2c6707cb653dca6370d6e8afc00ed5e240a7277001e67e6aa60ba7cbbbe1b3763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Bacterial proteins</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell division</topic><topic>cell growth</topic><topic>cell walls</topic><topic>cytoskeletal proteins</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Cytoskeleton</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - cytology</topic><topic>Escherichia coli - physiology</topic><topic>Fluid dynamics</topic><topic>genes</topic><topic>Hydrostatic Pressure</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>morphogenesis</topic><topic>Morphology</topic><topic>mutants</topic><topic>Mutation</topic><topic>pressure treatment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abe, Atsumu</creatorcontrib><creatorcontrib>Furukawa, Soichi</creatorcontrib><creatorcontrib>Migita, Yuya</creatorcontrib><creatorcontrib>Tanaka, Motoharu</creatorcontrib><creatorcontrib>Ogihara, Hirokazu</creatorcontrib><creatorcontrib>Morinaga, Yasushi</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abe, Atsumu</au><au>Furukawa, Soichi</au><au>Migita, Yuya</au><au>Tanaka, Motoharu</au><au>Ogihara, Hirokazu</au><au>Morinaga, Yasushi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sublethal High Hydrostatic Pressure Treatment Reveals the Importance of Genes Coding Cytoskeletal Protein in Escherichia Coli Morphogenesis</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>67</volume><issue>5</issue><spage>515</spage><epage>521</epage><pages>515-521</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>We studied morphologic changes after sublethal high hydrostatic pressure treatment (HPT) of Escherichia coli K-12 strains in which genes related to the cytoskeleton, cell wall, and cell division had been deleted. Some long filamentous and swelling cells were observed in wild-type bacteria, while some spherical, branched, or collapsed cells were observed in deletion mutants. In particular, ΔzapA and ΔrodZ showed distinguished morphologies. ZapA supports FtsZ, a cytoskeletal protein, forming ring with ZapB. RodZ, a cytoskeletal protein, interacts with MreB, also a cytoskeletal protein, and both factors are necessary for maintaining the rod shape of the cell. These results showed that insufficient formation of FtsZ rings induced cell elongation and that insufficient formation of MreB induced a branched and collapsed cell shape. Therefore, the correct formation of the bacteria cytoskeleton by FtsZ rings and MreB is important for keeping normal cell shape during growth after HPT, and the polymerization of cytoskeletal proteins was a critical target of sublethal HPT. These results indicate that sublethal HPT induces bacterial cell morphologic change and provide important information on the role of genes involved in morphogenesis. Therefore, sublethal HPT may be a good tool for studying the morphogenesis of bacterial cells.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><pmid>23708427</pmid><doi>10.1007/s00284-013-0392-8</doi><tpages>7</tpages></addata></record> |
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| subjects | Bacterial proteins Biomedical and Life Sciences Biotechnology Cell division cell growth cell walls cytoskeletal proteins Cytoskeletal Proteins - genetics Cytoskeletal Proteins - metabolism Cytoskeleton E coli Escherichia coli Escherichia coli - cytology Escherichia coli - physiology Fluid dynamics genes Hydrostatic Pressure Life Sciences Microbiology morphogenesis Morphology mutants Mutation pressure treatment |
| title | Sublethal High Hydrostatic Pressure Treatment Reveals the Importance of Genes Coding Cytoskeletal Protein in Escherichia Coli Morphogenesis |
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