The switch role of the Tmod4 in the regulation of balanced development between myogenesis and adipogenesis
Tmod4 (Tropomodulin 4) is a member of Tmod family that plays important role in thin filament length regulation and myofibril assembly. We found that the expression levels of Tmod4 were higher in skeletal muscle and adipose tissues. However, the function and regulation of the Tmod4 gene in the myogen...
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| Veröffentlicht in: | Gene 2013-12, Vol.532 (2), p.263-271 |
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| creator | Zhao, Xiao Huang, Zheng Liu, Xiaohong Chen, Yaosheng Gong, Wen Yu, Kaifan Qin, Lijun Chen, Hu Mo, Delin |
| description | Tmod4 (Tropomodulin 4) is a member of Tmod family that plays important role in thin filament length regulation and myofibril assembly. We found that the expression levels of Tmod4 were higher in skeletal muscle and adipose tissues. However, the function and regulation of the Tmod4 gene in the myogenesis and adipogenesis remains unclear. In this study, we found that the expression of Tmod4 was decreased in myogenesis while increased in adipogenesis. Then, the transcriptional regulation analysis of Tmod4 promoter showed that Tmod4 could be regulated directly by myogenic factors and adipogenic factors. Furthermore, the roles of Tmod4 in the myogenesis and adipogenesis were confirmed by its over-expression in C2C12 cells and 3T3 cells, which suggested that Tmod4 could promote adipogenesis by up-regulating the adipogenic factors but moderately delay the myogenesis. These results indicated that the Tmod4 gene may play as a switch between myogenesis and adipogenesis, which resulted in the balanced development between skeletal muscle and adipose tissue. Therefore, the model for switch role of the Tmod4 in the balanced regulation between myogenesis and adipogenesis was proposed. It is showed that the expression of Tmod4 was activated in adipogenesis by adipogenic factors while inhibited in myogenesis by myogenic factors. Moreover, Tmod4 could promote adipogenesis by up-regulating the expression of adipogenic factors while moderately delaying the myogenesis. Our study provides an important basis for further understanding the regulation and function of porcine Tmod4 in muscle and fat development.
•Tmod4 was up-regulated in adipogenesis while down-regulated in myogenesis.•Tmod4 could be regulated by adipogenic factors and myogenic factors.•Tmod4 could promote adipogenesis by up-regulating adipogenic factors.•Tmod4 could moderate delay the myogenesis.•Tmod4 gene may regulate a switch between myogenesis and adipogenesis. |
| doi_str_mv | 10.1016/j.gene.2013.08.088 |
| format | Article |
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•Tmod4 was up-regulated in adipogenesis while down-regulated in myogenesis.•Tmod4 could be regulated by adipogenic factors and myogenic factors.•Tmod4 could promote adipogenesis by up-regulating adipogenic factors.•Tmod4 could moderate delay the myogenesis.•Tmod4 gene may regulate a switch between myogenesis and adipogenesis.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2013.08.088</identifier><identifier>PMID: 24036428</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>3T3 Cells ; 3T3-L1 ; Adipogenesis ; Adipogenesis - genetics ; adipose tissue ; Adipose Tissue - growth & development ; Animals ; Base Sequence ; Binding Sites ; C2C12 ; Conserved Sequence ; Gene Expression Regulation, Developmental ; genes ; Mice ; Molecular Sequence Data ; muscle development ; Muscle Development - genetics ; muscles ; Myogenesis ; Organ Specificity ; Protein Binding ; Protein Transport ; Sequence Analysis, DNA ; skeletal muscle ; Sus scrofa - genetics ; Sus scrofa - growth & development ; swine ; Tmod4 ; transcription (genetics) ; Transcription regulation ; Transcription, Genetic ; Tropomodulin - physiology</subject><ispartof>Gene, 2013-12, Vol.532 (2), p.263-271</ispartof><rights>2013 Elsevier B.V.</rights><rights>2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-794a38860c4fe40cd71021b2c62bdbda6ab7f6c6f427ec421b9673e6f8eab3933</citedby><cites>FETCH-LOGICAL-c380t-794a38860c4fe40cd71021b2c62bdbda6ab7f6c6f427ec421b9673e6f8eab3933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gene.2013.08.088$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24036428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Xiao</creatorcontrib><creatorcontrib>Huang, Zheng</creatorcontrib><creatorcontrib>Liu, Xiaohong</creatorcontrib><creatorcontrib>Chen, Yaosheng</creatorcontrib><creatorcontrib>Gong, Wen</creatorcontrib><creatorcontrib>Yu, Kaifan</creatorcontrib><creatorcontrib>Qin, Lijun</creatorcontrib><creatorcontrib>Chen, Hu</creatorcontrib><creatorcontrib>Mo, Delin</creatorcontrib><title>The switch role of the Tmod4 in the regulation of balanced development between myogenesis and adipogenesis</title><title>Gene</title><addtitle>Gene</addtitle><description>Tmod4 (Tropomodulin 4) is a member of Tmod family that plays important role in thin filament length regulation and myofibril assembly. We found that the expression levels of Tmod4 were higher in skeletal muscle and adipose tissues. However, the function and regulation of the Tmod4 gene in the myogenesis and adipogenesis remains unclear. In this study, we found that the expression of Tmod4 was decreased in myogenesis while increased in adipogenesis. Then, the transcriptional regulation analysis of Tmod4 promoter showed that Tmod4 could be regulated directly by myogenic factors and adipogenic factors. Furthermore, the roles of Tmod4 in the myogenesis and adipogenesis were confirmed by its over-expression in C2C12 cells and 3T3 cells, which suggested that Tmod4 could promote adipogenesis by up-regulating the adipogenic factors but moderately delay the myogenesis. These results indicated that the Tmod4 gene may play as a switch between myogenesis and adipogenesis, which resulted in the balanced development between skeletal muscle and adipose tissue. Therefore, the model for switch role of the Tmod4 in the balanced regulation between myogenesis and adipogenesis was proposed. It is showed that the expression of Tmod4 was activated in adipogenesis by adipogenic factors while inhibited in myogenesis by myogenic factors. Moreover, Tmod4 could promote adipogenesis by up-regulating the expression of adipogenic factors while moderately delaying the myogenesis. Our study provides an important basis for further understanding the regulation and function of porcine Tmod4 in muscle and fat development.
•Tmod4 was up-regulated in adipogenesis while down-regulated in myogenesis.•Tmod4 could be regulated by adipogenic factors and myogenic factors.•Tmod4 could promote adipogenesis by up-regulating adipogenic factors.•Tmod4 could moderate delay the myogenesis.•Tmod4 gene may regulate a switch between myogenesis and adipogenesis.</description><subject>3T3 Cells</subject><subject>3T3-L1</subject><subject>Adipogenesis</subject><subject>Adipogenesis - genetics</subject><subject>adipose tissue</subject><subject>Adipose Tissue - growth & development</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>C2C12</subject><subject>Conserved Sequence</subject><subject>Gene Expression Regulation, Developmental</subject><subject>genes</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>muscle development</subject><subject>Muscle Development - genetics</subject><subject>muscles</subject><subject>Myogenesis</subject><subject>Organ Specificity</subject><subject>Protein Binding</subject><subject>Protein Transport</subject><subject>Sequence Analysis, DNA</subject><subject>skeletal muscle</subject><subject>Sus scrofa - genetics</subject><subject>Sus scrofa - growth & development</subject><subject>swine</subject><subject>Tmod4</subject><subject>transcription (genetics)</subject><subject>Transcription regulation</subject><subject>Transcription, Genetic</subject><subject>Tropomodulin - physiology</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYMoOj7-gAvN0k3HvCZNwY0MvkBw4bgOaXI7ZmibMeko_ntTR10aLoR773cPh4PQKSVTSqi8XE2X0MOUEcqnROVSO2hCVVkVhHC1iyaEl6qglFYH6DClFclvNmP76IAJwqVgaoJWi1fA6cMP9hXH0AIODR7yaNEFJ7Dvv5sIy01rBh_6cV2b1vQWHHbwDm1Yd9APuIbhA6DH3WcYTSWfsOkdNs6vfwfHaK8xbYKTn_8IvdzeLOb3xePT3cP8-rGwXJGhKCthuFKSWNGAINaVlDBaMytZ7WpnpKnLRlrZCFaCFXlVyZKDbBSYmlecH6GLre46hrcNpEF3Pllos2sIm6SpEIoxIlSZUbZFbQwpRWj0OvrOxE9NiR4z1is9utdjxpqoXCofnf3ob-oO3N_Jb6gZON8CjQnaLKNP-uU5K8xy_oILJjJxtSUg5_DuIepkPYyh-gh20C74_xx8AbkNl4s</recordid><startdate>20131215</startdate><enddate>20131215</enddate><creator>Zhao, Xiao</creator><creator>Huang, Zheng</creator><creator>Liu, Xiaohong</creator><creator>Chen, Yaosheng</creator><creator>Gong, Wen</creator><creator>Yu, Kaifan</creator><creator>Qin, Lijun</creator><creator>Chen, Hu</creator><creator>Mo, Delin</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131215</creationdate><title>The switch role of the Tmod4 in the regulation of balanced development between myogenesis and adipogenesis</title><author>Zhao, Xiao ; Huang, Zheng ; Liu, Xiaohong ; Chen, Yaosheng ; Gong, Wen ; Yu, Kaifan ; Qin, Lijun ; Chen, Hu ; Mo, Delin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-794a38860c4fe40cd71021b2c62bdbda6ab7f6c6f427ec421b9673e6f8eab3933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>3T3 Cells</topic><topic>3T3-L1</topic><topic>Adipogenesis</topic><topic>Adipogenesis - genetics</topic><topic>adipose tissue</topic><topic>Adipose Tissue - growth & development</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>C2C12</topic><topic>Conserved Sequence</topic><topic>Gene Expression Regulation, Developmental</topic><topic>genes</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>muscle development</topic><topic>Muscle Development - genetics</topic><topic>muscles</topic><topic>Myogenesis</topic><topic>Organ Specificity</topic><topic>Protein Binding</topic><topic>Protein Transport</topic><topic>Sequence Analysis, DNA</topic><topic>skeletal muscle</topic><topic>Sus scrofa - genetics</topic><topic>Sus scrofa - growth & development</topic><topic>swine</topic><topic>Tmod4</topic><topic>transcription (genetics)</topic><topic>Transcription regulation</topic><topic>Transcription, Genetic</topic><topic>Tropomodulin - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Xiao</creatorcontrib><creatorcontrib>Huang, Zheng</creatorcontrib><creatorcontrib>Liu, Xiaohong</creatorcontrib><creatorcontrib>Chen, Yaosheng</creatorcontrib><creatorcontrib>Gong, Wen</creatorcontrib><creatorcontrib>Yu, Kaifan</creatorcontrib><creatorcontrib>Qin, Lijun</creatorcontrib><creatorcontrib>Chen, Hu</creatorcontrib><creatorcontrib>Mo, Delin</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Xiao</au><au>Huang, Zheng</au><au>Liu, Xiaohong</au><au>Chen, Yaosheng</au><au>Gong, Wen</au><au>Yu, Kaifan</au><au>Qin, Lijun</au><au>Chen, Hu</au><au>Mo, Delin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The switch role of the Tmod4 in the regulation of balanced development between myogenesis and adipogenesis</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2013-12-15</date><risdate>2013</risdate><volume>532</volume><issue>2</issue><spage>263</spage><epage>271</epage><pages>263-271</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Tmod4 (Tropomodulin 4) is a member of Tmod family that plays important role in thin filament length regulation and myofibril assembly. We found that the expression levels of Tmod4 were higher in skeletal muscle and adipose tissues. However, the function and regulation of the Tmod4 gene in the myogenesis and adipogenesis remains unclear. In this study, we found that the expression of Tmod4 was decreased in myogenesis while increased in adipogenesis. Then, the transcriptional regulation analysis of Tmod4 promoter showed that Tmod4 could be regulated directly by myogenic factors and adipogenic factors. Furthermore, the roles of Tmod4 in the myogenesis and adipogenesis were confirmed by its over-expression in C2C12 cells and 3T3 cells, which suggested that Tmod4 could promote adipogenesis by up-regulating the adipogenic factors but moderately delay the myogenesis. These results indicated that the Tmod4 gene may play as a switch between myogenesis and adipogenesis, which resulted in the balanced development between skeletal muscle and adipose tissue. Therefore, the model for switch role of the Tmod4 in the balanced regulation between myogenesis and adipogenesis was proposed. It is showed that the expression of Tmod4 was activated in adipogenesis by adipogenic factors while inhibited in myogenesis by myogenic factors. Moreover, Tmod4 could promote adipogenesis by up-regulating the expression of adipogenic factors while moderately delaying the myogenesis. Our study provides an important basis for further understanding the regulation and function of porcine Tmod4 in muscle and fat development.
•Tmod4 was up-regulated in adipogenesis while down-regulated in myogenesis.•Tmod4 could be regulated by adipogenic factors and myogenic factors.•Tmod4 could promote adipogenesis by up-regulating adipogenic factors.•Tmod4 could moderate delay the myogenesis.•Tmod4 gene may regulate a switch between myogenesis and adipogenesis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24036428</pmid><doi>10.1016/j.gene.2013.08.088</doi><tpages>9</tpages></addata></record> |
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| ispartof | Gene, 2013-12, Vol.532 (2), p.263-271 |
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| subjects | 3T3 Cells 3T3-L1 Adipogenesis Adipogenesis - genetics adipose tissue Adipose Tissue - growth & development Animals Base Sequence Binding Sites C2C12 Conserved Sequence Gene Expression Regulation, Developmental genes Mice Molecular Sequence Data muscle development Muscle Development - genetics muscles Myogenesis Organ Specificity Protein Binding Protein Transport Sequence Analysis, DNA skeletal muscle Sus scrofa - genetics Sus scrofa - growth & development swine Tmod4 transcription (genetics) Transcription regulation Transcription, Genetic Tropomodulin - physiology |
| title | The switch role of the Tmod4 in the regulation of balanced development between myogenesis and adipogenesis |
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