Alteromonas australica sp. nov., isolated from the Tasman Sea

A non-pigmented, motile, Gram-negative bacterium designated H 17 T was isolated from a seawater sample collected in Port Phillip Bay (the Tasman Sea, Pacific Ocean). The new organism displayed optimal growth between 4 and 37 °C, was found to be neutrophilic and slightly halophilic, tolerating salt w...

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Veröffentlicht in:Antonie van Leeuwenhoek 2013-04, Vol.103 (4), p.877-884
Hauptverfasser: Ivanova, Elena P., Ng, Hooi Jun, Webb, Hayden K., Kurilenko, Valeriya V., Zhukova, Natalia V., Mikhailov, Valery V., Ponamoreva, Olga N., Crawford, Russell J.
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container_issue 4
container_start_page 877
container_title Antonie van Leeuwenhoek
container_volume 103
creator Ivanova, Elena P.
Ng, Hooi Jun
Webb, Hayden K.
Kurilenko, Valeriya V.
Zhukova, Natalia V.
Mikhailov, Valery V.
Ponamoreva, Olga N.
Crawford, Russell J.
description A non-pigmented, motile, Gram-negative bacterium designated H 17 T was isolated from a seawater sample collected in Port Phillip Bay (the Tasman Sea, Pacific Ocean). The new organism displayed optimal growth between 4 and 37 °C, was found to be neutrophilic and slightly halophilic, tolerating salt water environments up to 10 % NaCl. Strain H 17 T was found to be able to degrade starch and Tween 80 but unable to degrade gelatin or agar. Phosphatidylglycerol (27.7 %) and phosphatidylethanolamine (72.3 %) were found to be the only associated phospholipids. The major fatty acids identified are typical for the genus Alteromonas and include C 16:0 , C 16:1 ω7, C 17:1 ω8 and C 18:1 ω7. The G+C content of the DNA was found to be 43.4 mol%. A phylogenetic study, based on the 16S rRNA gene sequence analysis and Multilocus Phylogenetic Analysis, clearly indicated that strain H 17 T belongs to the genus Alteromonas . The DNA−DNA relatedness between strain H 17 T and the validly named Alteromonas species was between 30.7 and 46.4 mol%. Based on these results, a new species, Alteromonas australica , is proposed. The type strain is H 17 T (= KMM 6016 T  = CIP 109921 T ).
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The new organism displayed optimal growth between 4 and 37 °C, was found to be neutrophilic and slightly halophilic, tolerating salt water environments up to 10 % NaCl. Strain H 17 T was found to be able to degrade starch and Tween 80 but unable to degrade gelatin or agar. Phosphatidylglycerol (27.7 %) and phosphatidylethanolamine (72.3 %) were found to be the only associated phospholipids. The major fatty acids identified are typical for the genus Alteromonas and include C 16:0 , C 16:1 ω7, C 17:1 ω8 and C 18:1 ω7. The G+C content of the DNA was found to be 43.4 mol%. A phylogenetic study, based on the 16S rRNA gene sequence analysis and Multilocus Phylogenetic Analysis, clearly indicated that strain H 17 T belongs to the genus Alteromonas . The DNA−DNA relatedness between strain H 17 T and the validly named Alteromonas species was between 30.7 and 46.4 mol%. Based on these results, a new species, Alteromonas australica , is proposed. 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The new organism displayed optimal growth between 4 and 37 °C, was found to be neutrophilic and slightly halophilic, tolerating salt water environments up to 10 % NaCl. Strain H 17 T was found to be able to degrade starch and Tween 80 but unable to degrade gelatin or agar. Phosphatidylglycerol (27.7 %) and phosphatidylethanolamine (72.3 %) were found to be the only associated phospholipids. The major fatty acids identified are typical for the genus Alteromonas and include C 16:0 , C 16:1 ω7, C 17:1 ω8 and C 18:1 ω7. The G+C content of the DNA was found to be 43.4 mol%. A phylogenetic study, based on the 16S rRNA gene sequence analysis and Multilocus Phylogenetic Analysis, clearly indicated that strain H 17 T belongs to the genus Alteromonas . The DNA−DNA relatedness between strain H 17 T and the validly named Alteromonas species was between 30.7 and 46.4 mol%. Based on these results, a new species, Alteromonas australica , is proposed. 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The new organism displayed optimal growth between 4 and 37 °C, was found to be neutrophilic and slightly halophilic, tolerating salt water environments up to 10 % NaCl. Strain H 17 T was found to be able to degrade starch and Tween 80 but unable to degrade gelatin or agar. Phosphatidylglycerol (27.7 %) and phosphatidylethanolamine (72.3 %) were found to be the only associated phospholipids. The major fatty acids identified are typical for the genus Alteromonas and include C 16:0 , C 16:1 ω7, C 17:1 ω8 and C 18:1 ω7. The G+C content of the DNA was found to be 43.4 mol%. A phylogenetic study, based on the 16S rRNA gene sequence analysis and Multilocus Phylogenetic Analysis, clearly indicated that strain H 17 T belongs to the genus Alteromonas . The DNA−DNA relatedness between strain H 17 T and the validly named Alteromonas species was between 30.7 and 46.4 mol%. Based on these results, a new species, Alteromonas australica , is proposed. The type strain is H 17 T (= KMM 6016 T  = CIP 109921 T ).</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>23291832</pmid><doi>10.1007/s10482-012-9869-x</doi><tpages>8</tpages></addata></record>
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subjects Alteromonas
Alteromonas - classification
Alteromonas - genetics
Alteromonas - isolation & purification
Alteromonas - physiology
Bacterial Typing Techniques
Bacteriology
Base Composition
Biomedical and Life Sciences
Chemical analysis
Cluster Analysis
Deoxyribonucleic acid
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Fatty Acids - analysis
Gram-negative bacteria
Life Sciences
Marine environment
Medical Microbiology
Microbiology
Molecular Sequence Data
Nucleic Acid Hybridization
Oceans
Original Paper
Pacific Ocean
Phospholipids - analysis
Phylogenetics
Phylogeny
Plant Sciences
RNA, Ribosomal, 16S - genetics
Saline water
Seawater
Seawater - microbiology
Sequence Analysis, DNA
Sodium chloride
Soil Science & Conservation
Taxonomy
Water analysis
title Alteromonas australica sp. nov., isolated from the Tasman Sea
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