Measurement of Protein−Ligand Binding Constants from Reaction-Diffusion Concentration Profiles
Protein−ligand dissociation constants, K d, are determined precisely and down to the picomolar range from reaction-diffusion (RD) concentration profiles created by proteins diffusing through hydrogels functionalized with protein ligands. The RD process effectively amplifies the molecular-scale bindi...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2010-11, Vol.82 (21), p.8780-8784 |
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| container_end_page | 8784 |
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| container_issue | 21 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Wei, Yanhu Wesson, Paul J Kourkine, Igor Grzybowski, Bartosz A |
| description | Protein−ligand dissociation constants, K d, are determined precisely and down to the picomolar range from reaction-diffusion (RD) concentration profiles created by proteins diffusing through hydrogels functionalized with protein ligands. The RD process effectively amplifies the molecular-scale binding events into macroscopic patterns visible to the naked eye. The method is applicable to various protein−ligand pairs and does not require any prior knowledge about the protein structure. |
| doi_str_mv | 10.1021/ac102055a |
| format | Article |
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| ispartof | Analytical chemistry (Washington), 2010-11, Vol.82 (21), p.8780-8784 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Analytical chemistry Analytical, structural and metabolic biochemistry Binding sites Biological and medical sciences Chemical reactions Diffusion Fundamental and applied biological sciences. Psychology Gels - chemistry Kinetics Ligands Measurement Protein Binding Proteins Proteins - metabolism |
| title | Measurement of Protein−Ligand Binding Constants from Reaction-Diffusion Concentration Profiles |
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