Copper toxicity in the microalga Chlamydomonas reinhardtii: an integrated approach
The effects of copper exposure at five different concentrations on the freshwater alga Chlamydomonas reinhardtii were studied at the biochemical (metabolite), physiological (uptake kinetics and flow cytometry) and growth level. Changes at the physiological level were evident at the lowest exposure c...
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| Veröffentlicht in: | Biometals 2013-10, Vol.26 (5), p.731-740 |
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| creator | Jamers, An Blust, Ronny De Coen, Wim Griffin, Julian L. Jones, Oliver A. H. |
| description | The effects of copper exposure at five different concentrations on the freshwater alga
Chlamydomonas reinhardtii
were studied at the biochemical (metabolite), physiological (uptake kinetics and flow cytometry) and growth level. Changes at the physiological level were evident at the lowest exposure concentration while effects on the metabolome and on growth only occurred at the highest copper concentration tested. Flow cytometry revealed the presence of higher reactive oxygen species concentrations in algae exposed to higher copper concentrations and this was confirmed by a significant reduction in glutathione levels as part of the metabolomics assessment. Cu
2+
uptake kinetic data contributed information on possible mechanisms of copper toxicity, revealing that, a decrease in efflux pumping might be at the basis of an increased metal accumulation at higher exposure levels. This study demonstrates the value of using a comparative approach to investigating the mechanisms of toxicity rather than focusing on a single level of organization or effect. |
| doi_str_mv | 10.1007/s10534-013-9648-9 |
| format | Article |
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Chlamydomonas reinhardtii
were studied at the biochemical (metabolite), physiological (uptake kinetics and flow cytometry) and growth level. Changes at the physiological level were evident at the lowest exposure concentration while effects on the metabolome and on growth only occurred at the highest copper concentration tested. Flow cytometry revealed the presence of higher reactive oxygen species concentrations in algae exposed to higher copper concentrations and this was confirmed by a significant reduction in glutathione levels as part of the metabolomics assessment. Cu
2+
uptake kinetic data contributed information on possible mechanisms of copper toxicity, revealing that, a decrease in efflux pumping might be at the basis of an increased metal accumulation at higher exposure levels. This study demonstrates the value of using a comparative approach to investigating the mechanisms of toxicity rather than focusing on a single level of organization or effect.</description><identifier>ISSN: 0966-0844</identifier><identifier>EISSN: 1572-8773</identifier><identifier>DOI: 10.1007/s10534-013-9648-9</identifier><identifier>PMID: 23775669</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Algae ; Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Chlamydomonas reinhardtii ; Chlamydomonas reinhardtii - cytology ; Chlamydomonas reinhardtii - drug effects ; Copper ; Copper - chemistry ; Copper - toxicity ; Dose-Response Relationship, Drug ; Flow Cytometry ; Kinetics ; Life Sciences ; Medicine/Public Health ; Metabolites ; Microbiology ; Pharmacology/Toxicology ; Physiology ; Plant Physiology ; Structure-Activity Relationship ; Toxicity ; Toxicology</subject><ispartof>Biometals, 2013-10, Vol.26 (5), p.731-740</ispartof><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-66ffabaf587e51b2439182f7c47e8ebe326912eb459c2de3442dbea5a38fd31c3</citedby><cites>FETCH-LOGICAL-c405t-66ffabaf587e51b2439182f7c47e8ebe326912eb459c2de3442dbea5a38fd31c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10534-013-9648-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10534-013-9648-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23775669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jamers, An</creatorcontrib><creatorcontrib>Blust, Ronny</creatorcontrib><creatorcontrib>De Coen, Wim</creatorcontrib><creatorcontrib>Griffin, Julian L.</creatorcontrib><creatorcontrib>Jones, Oliver A. H.</creatorcontrib><title>Copper toxicity in the microalga Chlamydomonas reinhardtii: an integrated approach</title><title>Biometals</title><addtitle>Biometals</addtitle><addtitle>Biometals</addtitle><description>The effects of copper exposure at five different concentrations on the freshwater alga
Chlamydomonas reinhardtii
were studied at the biochemical (metabolite), physiological (uptake kinetics and flow cytometry) and growth level. Changes at the physiological level were evident at the lowest exposure concentration while effects on the metabolome and on growth only occurred at the highest copper concentration tested. Flow cytometry revealed the presence of higher reactive oxygen species concentrations in algae exposed to higher copper concentrations and this was confirmed by a significant reduction in glutathione levels as part of the metabolomics assessment. Cu
2+
uptake kinetic data contributed information on possible mechanisms of copper toxicity, revealing that, a decrease in efflux pumping might be at the basis of an increased metal accumulation at higher exposure levels. This study demonstrates the value of using a comparative approach to investigating the mechanisms of toxicity rather than focusing on a single level of organization or effect.</description><subject>Algae</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Chlamydomonas reinhardtii</subject><subject>Chlamydomonas reinhardtii - cytology</subject><subject>Chlamydomonas reinhardtii - drug effects</subject><subject>Copper</subject><subject>Copper - chemistry</subject><subject>Copper - toxicity</subject><subject>Dose-Response Relationship, Drug</subject><subject>Flow Cytometry</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Medicine/Public Health</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>Pharmacology/Toxicology</subject><subject>Physiology</subject><subject>Plant Physiology</subject><subject>Structure-Activity Relationship</subject><subject>Toxicity</subject><subject>Toxicology</subject><issn>0966-0844</issn><issn>1572-8773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kMtKxDAUhoMozjj6AG6k4MZNNbcmjTsp3mBAEF2HtD2dydCbSQvO25uho4jg6izy_f85-RA6J_iaYCxvPMEJ4zEmLFaCp7E6QHOSSBqnUrJDNMdKiBinnM_QifcbjLGSWByjGWVSJkKoOXrNur4HFw3dpy3ssI1sGw1riBpbuM7UKxNl69o027Jrutb4yIFt18aVg7W3kWkDPsDKmQHKyPR9iBTrU3RUmdrD2X4u0PvD_Vv2FC9fHp-zu2VccJwMsRBVZXJTJamEhOSUM0VSWsmCS0ghB0aFIhRynqiClsA4p2UOJjEsrUpGCrZAV1NvWPsxgh90Y30BdW1a6EavCecpJUxJGtDLP-imG10brgsUY5RSrHigyESFr3vvoNK9s41xW02w3gnXk3AdhOudcK1C5mLfPOYNlD-Jb8MBoBPgw1O7Avdr9b-tX1Gli5I</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Jamers, An</creator><creator>Blust, Ronny</creator><creator>De Coen, Wim</creator><creator>Griffin, Julian L.</creator><creator>Jones, Oliver A. 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H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-66ffabaf587e51b2439182f7c47e8ebe326912eb459c2de3442dbea5a38fd31c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Algae</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Chlamydomonas reinhardtii</topic><topic>Chlamydomonas reinhardtii - cytology</topic><topic>Chlamydomonas reinhardtii - drug effects</topic><topic>Copper</topic><topic>Copper - chemistry</topic><topic>Copper - toxicity</topic><topic>Dose-Response Relationship, Drug</topic><topic>Flow Cytometry</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Medicine/Public Health</topic><topic>Metabolites</topic><topic>Microbiology</topic><topic>Pharmacology/Toxicology</topic><topic>Physiology</topic><topic>Plant Physiology</topic><topic>Structure-Activity Relationship</topic><topic>Toxicity</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jamers, An</creatorcontrib><creatorcontrib>Blust, Ronny</creatorcontrib><creatorcontrib>De Coen, Wim</creatorcontrib><creatorcontrib>Griffin, Julian L.</creatorcontrib><creatorcontrib>Jones, Oliver A. 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H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Copper toxicity in the microalga Chlamydomonas reinhardtii: an integrated approach</atitle><jtitle>Biometals</jtitle><stitle>Biometals</stitle><addtitle>Biometals</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>26</volume><issue>5</issue><spage>731</spage><epage>740</epage><pages>731-740</pages><issn>0966-0844</issn><eissn>1572-8773</eissn><abstract>The effects of copper exposure at five different concentrations on the freshwater alga
Chlamydomonas reinhardtii
were studied at the biochemical (metabolite), physiological (uptake kinetics and flow cytometry) and growth level. Changes at the physiological level were evident at the lowest exposure concentration while effects on the metabolome and on growth only occurred at the highest copper concentration tested. Flow cytometry revealed the presence of higher reactive oxygen species concentrations in algae exposed to higher copper concentrations and this was confirmed by a significant reduction in glutathione levels as part of the metabolomics assessment. Cu
2+
uptake kinetic data contributed information on possible mechanisms of copper toxicity, revealing that, a decrease in efflux pumping might be at the basis of an increased metal accumulation at higher exposure levels. This study demonstrates the value of using a comparative approach to investigating the mechanisms of toxicity rather than focusing on a single level of organization or effect.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>23775669</pmid><doi>10.1007/s10534-013-9648-9</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| language | eng |
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| subjects | Algae Biochemistry Biomedical and Life Sciences Cell Biology Chlamydomonas reinhardtii Chlamydomonas reinhardtii - cytology Chlamydomonas reinhardtii - drug effects Copper Copper - chemistry Copper - toxicity Dose-Response Relationship, Drug Flow Cytometry Kinetics Life Sciences Medicine/Public Health Metabolites Microbiology Pharmacology/Toxicology Physiology Plant Physiology Structure-Activity Relationship Toxicity Toxicology |
| title | Copper toxicity in the microalga Chlamydomonas reinhardtii: an integrated approach |
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