Determination of chokeberry (Aronia melanocarpa) polyphenol components using liquid chromatography–tandem mass spectrometry: Overall contribution to antioxidant activity

•Polyphenols in Korean chokeberry leaves were determined by HPLC–MS/MS.•Six polyphenols were characterised in the leaves for the first time.•The antioxidant activity of leaf extracts were evaluated. The type and content of plant polyphenols can be influenced by maturity. Korean chokeberry (Aronia me...

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Veröffentlicht in:Food chemistry 2014-03, Vol.146, p.1-5
Hauptverfasser: Lee, Ji Eun, Kim, Gon-Sup, Park, Semin, Kim, Yun-Hi, Kim, Man-Bae, Lee, Won Sup, Jeong, Sung Woo, Lee, Soo Jung, Jin, Jong Sung, Shin, Sung Chul
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container_issue
container_start_page 1
container_title Food chemistry
container_volume 146
creator Lee, Ji Eun
Kim, Gon-Sup
Park, Semin
Kim, Yun-Hi
Kim, Man-Bae
Lee, Won Sup
Jeong, Sung Woo
Lee, Soo Jung
Jin, Jong Sung
Shin, Sung Chul
description •Polyphenols in Korean chokeberry leaves were determined by HPLC–MS/MS.•Six polyphenols were characterised in the leaves for the first time.•The antioxidant activity of leaf extracts were evaluated. The type and content of plant polyphenols can be influenced by maturity. Korean chokeberry (Aronia melanocarpa) leaves of three different maturities (young, mature, and aged) were extracted with 70% aqueous methanol. The polyphenols in the leaves were analysed for the first time using high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) and comparison with reported data. Among the 12 characterised components, five flavonoids, 3, 4, and 10–12, and a dicaffeoylquinic acid derivative, 6, were characterised for the first time in chokeberry leaves. Each polyphenol component was validated and quantified using a representative polyphenol standard of the same group. The antioxidant activity of the three different mature leaf extracts was determined. The antioxidant activity was highest for young leaves, followed by mature and aged leaves. The results suggest that younger chokeberry leaves may be more favourable for processing a higher quality functional tea due to their higher polyphenol content.
doi_str_mv 10.1016/j.foodchem.2013.09.029
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The type and content of plant polyphenols can be influenced by maturity. Korean chokeberry (Aronia melanocarpa) leaves of three different maturities (young, mature, and aged) were extracted with 70% aqueous methanol. The polyphenols in the leaves were analysed for the first time using high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) and comparison with reported data. Among the 12 characterised components, five flavonoids, 3, 4, and 10–12, and a dicaffeoylquinic acid derivative, 6, were characterised for the first time in chokeberry leaves. Each polyphenol component was validated and quantified using a representative polyphenol standard of the same group. The antioxidant activity of the three different mature leaf extracts was determined. The antioxidant activity was highest for young leaves, followed by mature and aged leaves. 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subjects Antioxidant effect
Antioxidants - chemistry
Biological and medical sciences
Chokeberry
Chromatography, High Pressure Liquid
Food toxicology
Functional food
Liquid chromatography
Medical sciences
Molecular Structure
Photinia - chemistry
Photinia - growth & development
Plant Extracts - chemistry
Plant Leaves - chemistry
Plant Leaves - growth & development
Polyphenol
Polyphenols - chemistry
Tandem mass spectrometry
Tandem Mass Spectrometry - methods
Toxicology
title Determination of chokeberry (Aronia melanocarpa) polyphenol components using liquid chromatography–tandem mass spectrometry: Overall contribution to antioxidant activity
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