Strain specific differences in ribosomal DNA from the fungus Schizophyllum commune
CsCl-bisbenzimide gradients were used to purify ribosomal DNA (rDNA) from Schizophyllum commune total DNA. Southern hybridizations demonstrate that this DNA codes for rRNA. Restriction mapping of the rDNA from four strains revealed strain variation with repeat lengths of 9.2-9.6 kbp. Specific differ...
Gespeichert in:
| Veröffentlicht in: | Current genetics 1984-04, Vol.8 (3), p.219-222 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 222 |
|---|---|
| container_issue | 3 |
| container_start_page | 219 |
| container_title | Current genetics |
| container_volume | 8 |
| creator | Specht, C.A Novotny, C.P Ullrich, R.C |
| description | CsCl-bisbenzimide gradients were used to purify ribosomal DNA (rDNA) from Schizophyllum commune total DNA. Southern hybridizations demonstrate that this DNA codes for rRNA. Restriction mapping of the rDNA from four strains revealed strain variation with repeat lengths of 9.2-9.6 kbp. Specific differences in the length of the rDNA repeat in different strains are due to insertions of 0.2 or 0.4 kbp of DNA at a single site. Different strains also show restriction site polymorphisms. Our analysis demonstrates the caution that must be exercised when interpreting restriction data from genomes containing restriction polymorphisms. Restriction digests with MspI and HpaII indicate that the rDNA contains 5-methylcytosine and that the unit repeats are not methylated identically, but rather differentially. This is the first report of methylated rDNA in fungi. |
| doi_str_mv | 10.1007/BF00417819 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1448208383</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1448208383</sourcerecordid><originalsourceid>FETCH-LOGICAL-c369t-e58a0034510dc2e235eb952dd47b077f4377138b4052c6ee73d0f86e536362003</originalsourceid><addsrcrecordid>eNp90E1v1DAQBmALUdFt4cIPAB9QhSoFxl-xfSyFFqQKJJaeI8cZd42SeLE3h_LrMdqlvXEaafTMK81LyEsG7xiAfv_hCkAybZh9QlZMCt6ANeIpWQHTvDFgxDE5KeUnAOPG6mfkmFeuteQr8n29yy7OtGzRxxA9HWIImHH2WGjd59inkiY30o9fL2jIaaK7DdKwzHdLoWu_ib_TdnM_jstEfZqmZcbn5Ci4seCLwzwlt1efflx-bm6-XX-5vLhpvGjtrkFlHICQisHgOXKhsLeKD4PUPWgdpNCaCdNLUNy3iFoMEEyLSrSi5fXylLzd525z-rVg2XVTLB7H0c2YltIxKQ2vzxtR6dn_qbDSCmUrPN9Dn1MpGUO3zXFy-b5j0P0tu3ssu-JXh9Sln3B4oP_areDNAbji3Riym30sD85qDpKryl7vWXCpc3e5kts1ByaAq9bwVog_c8WNXw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13949359</pqid></control><display><type>article</type><title>Strain specific differences in ribosomal DNA from the fungus Schizophyllum commune</title><source>SpringerNature Journals</source><creator>Specht, C.A ; Novotny, C.P ; Ullrich, R.C</creator><creatorcontrib>Specht, C.A ; Novotny, C.P ; Ullrich, R.C</creatorcontrib><description>CsCl-bisbenzimide gradients were used to purify ribosomal DNA (rDNA) from Schizophyllum commune total DNA. Southern hybridizations demonstrate that this DNA codes for rRNA. Restriction mapping of the rDNA from four strains revealed strain variation with repeat lengths of 9.2-9.6 kbp. Specific differences in the length of the rDNA repeat in different strains are due to insertions of 0.2 or 0.4 kbp of DNA at a single site. Different strains also show restriction site polymorphisms. Our analysis demonstrates the caution that must be exercised when interpreting restriction data from genomes containing restriction polymorphisms. Restriction digests with MspI and HpaII indicate that the rDNA contains 5-methylcytosine and that the unit repeats are not methylated identically, but rather differentially. This is the first report of methylated rDNA in fungi.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/BF00417819</identifier><identifier>PMID: 24177742</identifier><identifier>CODEN: CUGED5</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Molecular and cellular biology ; Molecular genetics ; plant breeding ; plant genetics ; Schizophyllum commune</subject><ispartof>Current genetics, 1984-04, Vol.8 (3), p.219-222</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-e58a0034510dc2e235eb952dd47b077f4377138b4052c6ee73d0f86e536362003</citedby><cites>FETCH-LOGICAL-c369t-e58a0034510dc2e235eb952dd47b077f4377138b4052c6ee73d0f86e536362003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9720425$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24177742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Specht, C.A</creatorcontrib><creatorcontrib>Novotny, C.P</creatorcontrib><creatorcontrib>Ullrich, R.C</creatorcontrib><title>Strain specific differences in ribosomal DNA from the fungus Schizophyllum commune</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>CsCl-bisbenzimide gradients were used to purify ribosomal DNA (rDNA) from Schizophyllum commune total DNA. Southern hybridizations demonstrate that this DNA codes for rRNA. Restriction mapping of the rDNA from four strains revealed strain variation with repeat lengths of 9.2-9.6 kbp. Specific differences in the length of the rDNA repeat in different strains are due to insertions of 0.2 or 0.4 kbp of DNA at a single site. Different strains also show restriction site polymorphisms. Our analysis demonstrates the caution that must be exercised when interpreting restriction data from genomes containing restriction polymorphisms. Restriction digests with MspI and HpaII indicate that the rDNA contains 5-methylcytosine and that the unit repeats are not methylated identically, but rather differentially. This is the first report of methylated rDNA in fungi.</description><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>plant breeding</subject><subject>plant genetics</subject><subject>Schizophyllum commune</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNp90E1v1DAQBmALUdFt4cIPAB9QhSoFxl-xfSyFFqQKJJaeI8cZd42SeLE3h_LrMdqlvXEaafTMK81LyEsG7xiAfv_hCkAybZh9QlZMCt6ANeIpWQHTvDFgxDE5KeUnAOPG6mfkmFeuteQr8n29yy7OtGzRxxA9HWIImHH2WGjd59inkiY30o9fL2jIaaK7DdKwzHdLoWu_ib_TdnM_jstEfZqmZcbn5Ci4seCLwzwlt1efflx-bm6-XX-5vLhpvGjtrkFlHICQisHgOXKhsLeKD4PUPWgdpNCaCdNLUNy3iFoMEEyLSrSi5fXylLzd525z-rVg2XVTLB7H0c2YltIxKQ2vzxtR6dn_qbDSCmUrPN9Dn1MpGUO3zXFy-b5j0P0tu3ssu-JXh9Sln3B4oP_areDNAbji3Riym30sD85qDpKryl7vWXCpc3e5kts1ByaAq9bwVog_c8WNXw</recordid><startdate>198404</startdate><enddate>198404</enddate><creator>Specht, C.A</creator><creator>Novotny, C.P</creator><creator>Ullrich, R.C</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198404</creationdate><title>Strain specific differences in ribosomal DNA from the fungus Schizophyllum commune</title><author>Specht, C.A ; Novotny, C.P ; Ullrich, R.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-e58a0034510dc2e235eb952dd47b077f4377138b4052c6ee73d0f86e536362003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>plant breeding</topic><topic>plant genetics</topic><topic>Schizophyllum commune</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Specht, C.A</creatorcontrib><creatorcontrib>Novotny, C.P</creatorcontrib><creatorcontrib>Ullrich, R.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Specht, C.A</au><au>Novotny, C.P</au><au>Ullrich, R.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Strain specific differences in ribosomal DNA from the fungus Schizophyllum commune</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1984-04</date><risdate>1984</risdate><volume>8</volume><issue>3</issue><spage>219</spage><epage>222</epage><pages>219-222</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><coden>CUGED5</coden><abstract>CsCl-bisbenzimide gradients were used to purify ribosomal DNA (rDNA) from Schizophyllum commune total DNA. Southern hybridizations demonstrate that this DNA codes for rRNA. Restriction mapping of the rDNA from four strains revealed strain variation with repeat lengths of 9.2-9.6 kbp. Specific differences in the length of the rDNA repeat in different strains are due to insertions of 0.2 or 0.4 kbp of DNA at a single site. Different strains also show restriction site polymorphisms. Our analysis demonstrates the caution that must be exercised when interpreting restriction data from genomes containing restriction polymorphisms. Restriction digests with MspI and HpaII indicate that the rDNA contains 5-methylcytosine and that the unit repeats are not methylated identically, but rather differentially. This is the first report of methylated rDNA in fungi.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>24177742</pmid><doi>10.1007/BF00417819</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1984-04, Vol.8 (3), p.219-222 |
| issn | 0172-8083 1432-0983 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1448208383 |
| source | SpringerNature Journals |
| subjects | Biological and medical sciences Fundamental and applied biological sciences. Psychology Genes. Genome Molecular and cellular biology Molecular genetics plant breeding plant genetics Schizophyllum commune |
| title | Strain specific differences in ribosomal DNA from the fungus Schizophyllum commune |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T11%3A20%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Strain%20specific%20differences%20in%20ribosomal%20DNA%20from%20the%20fungus%20Schizophyllum%20commune&rft.jtitle=Current%20genetics&rft.au=Specht,%20C.A&rft.date=1984-04&rft.volume=8&rft.issue=3&rft.spage=219&rft.epage=222&rft.pages=219-222&rft.issn=0172-8083&rft.eissn=1432-0983&rft.coden=CUGED5&rft_id=info:doi/10.1007/BF00417819&rft_dat=%3Cproquest_cross%3E1448208383%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13949359&rft_id=info:pmid/24177742&rfr_iscdi=true |