An improve method for somatic embryogenesis of Schisandra chinensis (Turcz.) Baillon
A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.0 mg L(-1) N-phenyl-1,2,3-thidiazol-5-yl-urea (TDZ) or 2.0 mg L...
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| Veröffentlicht in: | Pakistan journal of biological sciences 2013-02, Vol.16 (3), p.127-134 |
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| container_title | Pakistan journal of biological sciences |
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| creator | Sun, Dan Lang, Wen-Xiang Li, Hong-Bo Guo, Xiu-Wu Lian, Mei-Lan Piao, Zhong-Yun |
| description | A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.0 mg L(-1) N-phenyl-1,2,3-thidiazol-5-yl-urea (TDZ) or 2.0 mg L(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Addition of Zeatin (Zt) promoted the formation of embryogenic calli. To induce somatic embryogenesis, 2,4-D, TDZ and Zt were incorporated in the medium alone or in combination. Development of the maximum number of somatic embryos (81 globular, 37 heart, 52 torpedo and 37 cotyledon-stage) and germination of the highest number of embryos (50%) was observed on 1/2 MS medium supplemented with 1.0 mg L(-1) TDZ and 0.2 mg L(-1) Zt. Further development of somatic embryos into plantlets was completed in 1/2 MS medium free of plant growth regulators. |
| doi_str_mv | 10.3923/pjbs.2013.127.134 |
| format | Article |
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Baillon</title><source>Science Alert Free</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Sun, Dan ; Lang, Wen-Xiang ; Li, Hong-Bo ; Guo, Xiu-Wu ; Lian, Mei-Lan ; Piao, Zhong-Yun</creator><creatorcontrib>Sun, Dan ; Lang, Wen-Xiang ; Li, Hong-Bo ; Guo, Xiu-Wu ; Lian, Mei-Lan ; Piao, Zhong-Yun</creatorcontrib><description>A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.0 mg L(-1) N-phenyl-1,2,3-thidiazol-5-yl-urea (TDZ) or 2.0 mg L(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Addition of Zeatin (Zt) promoted the formation of embryogenic calli. To induce somatic embryogenesis, 2,4-D, TDZ and Zt were incorporated in the medium alone or in combination. Development of the maximum number of somatic embryos (81 globular, 37 heart, 52 torpedo and 37 cotyledon-stage) and germination of the highest number of embryos (50%) was observed on 1/2 MS medium supplemented with 1.0 mg L(-1) TDZ and 0.2 mg L(-1) Zt. Further development of somatic embryos into plantlets was completed in 1/2 MS medium free of plant growth regulators.</description><identifier>ISSN: 1028-8880</identifier><identifier>DOI: 10.3923/pjbs.2013.127.134</identifier><identifier>PMID: 24171274</identifier><language>eng</language><publisher>Pakistan</publisher><subject>2,4-Dichlorophenoxyacetic Acid - chemistry ; Culture Media - chemistry ; Germination ; Phenylurea Compounds - chemistry ; Plant Growth Regulators - chemistry ; Plant Shoots - growth & development ; Plant Somatic Embryogenesis Techniques - methods ; Schisandra - growth & development ; Seeds - growth & development ; Thiadiazoles - chemistry ; Zeatin - chemistry</subject><ispartof>Pakistan journal of biological sciences, 2013-02, Vol.16 (3), p.127-134</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c216t-a69498829919c399ef35e41e9bfe2036fe2d7aa023e745260fafb56e1fb89e4f3</citedby><cites>FETCH-LOGICAL-c216t-a69498829919c399ef35e41e9bfe2036fe2d7aa023e745260fafb56e1fb89e4f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4124,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24171274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Dan</creatorcontrib><creatorcontrib>Lang, Wen-Xiang</creatorcontrib><creatorcontrib>Li, Hong-Bo</creatorcontrib><creatorcontrib>Guo, Xiu-Wu</creatorcontrib><creatorcontrib>Lian, Mei-Lan</creatorcontrib><creatorcontrib>Piao, Zhong-Yun</creatorcontrib><title>An improve method for somatic embryogenesis of Schisandra chinensis (Turcz.) Baillon</title><title>Pakistan journal of biological sciences</title><addtitle>Pak J Biol Sci</addtitle><description>A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.0 mg L(-1) N-phenyl-1,2,3-thidiazol-5-yl-urea (TDZ) or 2.0 mg L(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Addition of Zeatin (Zt) promoted the formation of embryogenic calli. To induce somatic embryogenesis, 2,4-D, TDZ and Zt were incorporated in the medium alone or in combination. Development of the maximum number of somatic embryos (81 globular, 37 heart, 52 torpedo and 37 cotyledon-stage) and germination of the highest number of embryos (50%) was observed on 1/2 MS medium supplemented with 1.0 mg L(-1) TDZ and 0.2 mg L(-1) Zt. Further development of somatic embryos into plantlets was completed in 1/2 MS medium free of plant growth regulators.</description><subject>2,4-Dichlorophenoxyacetic Acid - chemistry</subject><subject>Culture Media - chemistry</subject><subject>Germination</subject><subject>Phenylurea Compounds - chemistry</subject><subject>Plant Growth Regulators - chemistry</subject><subject>Plant Shoots - growth & development</subject><subject>Plant Somatic Embryogenesis Techniques - methods</subject><subject>Schisandra - growth & development</subject><subject>Seeds - growth & development</subject><subject>Thiadiazoles - chemistry</subject><subject>Zeatin - chemistry</subject><issn>1028-8880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwzAQhH0A0VL4AVyQj-WQ4FcT-1gqXlIlDpSz5SRrmiqJi50glV-PoxYuu6vVzGj0IXRDScoV4_f7XRFSRihPKctTysUZmlLCZCKlJBN0GcKOEJGzXF6gCRM0jzIxRZtlh-t279034Bb6rauwdR4H15q-LjG0hT-4T-gg1AE7i9_LbR1MV3mD49VBN_7nm8GXP-kdfjB107juCp1b0wS4Pu0Z-nh63KxekvXb8-tquU5KRrM-MZkSSkqmFFUlVwosX4CgoAoLjPAszio3hjAOuViwjFhji0UG1BZSgbB8hubH3Nj_a4DQ67YOJTSN6cANQVMhJCOSMhml9CgtvQvBg9V7X7fGHzQlegSoR4B6BKgjGR0BRs_tKX4oWqj-HX_0-C8pEm7e</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Sun, Dan</creator><creator>Lang, Wen-Xiang</creator><creator>Li, Hong-Bo</creator><creator>Guo, Xiu-Wu</creator><creator>Lian, Mei-Lan</creator><creator>Piao, Zhong-Yun</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130201</creationdate><title>An improve method for somatic embryogenesis of Schisandra chinensis (Turcz.) Baillon</title><author>Sun, Dan ; Lang, Wen-Xiang ; Li, Hong-Bo ; Guo, Xiu-Wu ; Lian, Mei-Lan ; Piao, Zhong-Yun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c216t-a69498829919c399ef35e41e9bfe2036fe2d7aa023e745260fafb56e1fb89e4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>2,4-Dichlorophenoxyacetic Acid - chemistry</topic><topic>Culture Media - chemistry</topic><topic>Germination</topic><topic>Phenylurea Compounds - chemistry</topic><topic>Plant Growth Regulators - chemistry</topic><topic>Plant Shoots - growth & development</topic><topic>Plant Somatic Embryogenesis Techniques - methods</topic><topic>Schisandra - growth & development</topic><topic>Seeds - growth & development</topic><topic>Thiadiazoles - chemistry</topic><topic>Zeatin - chemistry</topic><toplevel>online_resources</toplevel><creatorcontrib>Sun, Dan</creatorcontrib><creatorcontrib>Lang, Wen-Xiang</creatorcontrib><creatorcontrib>Li, Hong-Bo</creatorcontrib><creatorcontrib>Guo, Xiu-Wu</creatorcontrib><creatorcontrib>Lian, Mei-Lan</creatorcontrib><creatorcontrib>Piao, Zhong-Yun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pakistan journal of biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Dan</au><au>Lang, Wen-Xiang</au><au>Li, Hong-Bo</au><au>Guo, Xiu-Wu</au><au>Lian, Mei-Lan</au><au>Piao, Zhong-Yun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improve method for somatic embryogenesis of Schisandra chinensis (Turcz.) Baillon</atitle><jtitle>Pakistan journal of biological sciences</jtitle><addtitle>Pak J Biol Sci</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>16</volume><issue>3</issue><spage>127</spage><epage>134</epage><pages>127-134</pages><issn>1028-8880</issn><abstract>A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.0 mg L(-1) N-phenyl-1,2,3-thidiazol-5-yl-urea (TDZ) or 2.0 mg L(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Addition of Zeatin (Zt) promoted the formation of embryogenic calli. To induce somatic embryogenesis, 2,4-D, TDZ and Zt were incorporated in the medium alone or in combination. Development of the maximum number of somatic embryos (81 globular, 37 heart, 52 torpedo and 37 cotyledon-stage) and germination of the highest number of embryos (50%) was observed on 1/2 MS medium supplemented with 1.0 mg L(-1) TDZ and 0.2 mg L(-1) Zt. Further development of somatic embryos into plantlets was completed in 1/2 MS medium free of plant growth regulators.</abstract><cop>Pakistan</cop><pmid>24171274</pmid><doi>10.3923/pjbs.2013.127.134</doi><tpages>8</tpages></addata></record> |
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| ispartof | Pakistan journal of biological sciences, 2013-02, Vol.16 (3), p.127-134 |
| issn | 1028-8880 |
| language | eng |
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| source | Science Alert Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 2,4-Dichlorophenoxyacetic Acid - chemistry Culture Media - chemistry Germination Phenylurea Compounds - chemistry Plant Growth Regulators - chemistry Plant Shoots - growth & development Plant Somatic Embryogenesis Techniques - methods Schisandra - growth & development Seeds - growth & development Thiadiazoles - chemistry Zeatin - chemistry |
| title | An improve method for somatic embryogenesis of Schisandra chinensis (Turcz.) Baillon |
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