The characterization of normal thyroid tissue by micro-FTIR spectroscopy
In this paper, we report the spectral patterns of normal human thyroid tissue and methodology to interpret hyperspectral imaging data and protein conformational changes observed therein. Raw image datasets were imported into software written in-house in the MATLAB environment and processed to yield...
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| Veröffentlicht in: | Analyst (London) 2013-12, Vol.138 (23), p.794-71 |
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| creator | Pereira, Thiago M Zezell, Denise M Bird, Benjamin Miljkovi, Milos Diem, Max |
| description | In this paper, we report the spectral patterns of normal human thyroid tissue and methodology to interpret hyperspectral imaging data and protein conformational changes observed therein. Raw image datasets were imported into software written in-house in the MATLAB environment and processed to yield pseudo-color images of the tissue sections. All spectra were vector normalized, noise-filtered, and corrected for water-vapour contributions and scattering effects before being subjected to Hierarchical Cluster Analysis (HCA) and correlated with histological structures obtained from images of H&E-stained parallel tissue sections. We successfully identified a protein structural heterogeneity that can be correlated with the spatially resolved amount of iodine in the thyroglobulin structure of colloids and follicular cells.
In this paper, we report the spectral patterns of normal human thyroid tissue and methodology to interpret hyperspectral imaging data and any protein conformational changes observed therein. |
| doi_str_mv | 10.1039/c3an00296a |
| format | Article |
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In this paper, we report the spectral patterns of normal human thyroid tissue and methodology to interpret hyperspectral imaging data and any protein conformational changes observed therein.</description><subject>Computer programs</subject><subject>Correlation</subject><subject>Heterogeneity</subject><subject>Humans</subject><subject>Matlab</subject><subject>Proteins</subject><subject>Spectra</subject><subject>Spectroscopy, Fourier Transform Infrared - methods</subject><subject>Thyroid Gland - chemistry</subject><subject>Thyroid Hormones - analysis</subject><subject>Tissue Array Analysis</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1Lw0AQxRdRbK1evCvrTYTozn4lOUqxtlAQpJ7DZrNLI0k27qaH-Ncbaa14US8zDO_HY2YeQudAboGw9E4z1RBCU6kO0BiY5JEQNDlEY0IIi6gUfIROQngdRiCCHKMR5cBiCWKM5qu1wXqtvNKd8eW76krXYGdx43ytKtyte-_KAndlCBuD8x7XpfYumq0Wzzi0RnfeBe3a_hQdWVUFc7brE_Qye1hN59Hy6XExvV9Gmsu4iwzNCbWMF4JTWyTCDlsnseCa5KnIZa4pL4iGQsrYUiFUKgzlxoK2VkgJkk3Q9da39e5tY0KX1WXQpqpUY9wmZBAPR0ICAH-jXHIh-VD_gfIYSAxJOqA3W3R4Qwje2Kz1Za18nwHJPvPIvvMY4Mud7yavTbFHvwIYgIst4IPeqz8Mrn7Ts7aw7AO5ZJm_</recordid><startdate>20131207</startdate><enddate>20131207</enddate><creator>Pereira, Thiago M</creator><creator>Zezell, Denise M</creator><creator>Bird, Benjamin</creator><creator>Miljkovi, Milos</creator><creator>Diem, Max</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20131207</creationdate><title>The characterization of normal thyroid tissue by micro-FTIR spectroscopy</title><author>Pereira, Thiago M ; Zezell, Denise M ; Bird, Benjamin ; Miljkovi, Milos ; Diem, Max</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-e2b02f34d542fd85f96a8754c0b95b6bc24d0c1d667f255a95e24ef1cff566163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Computer programs</topic><topic>Correlation</topic><topic>Heterogeneity</topic><topic>Humans</topic><topic>Matlab</topic><topic>Proteins</topic><topic>Spectra</topic><topic>Spectroscopy, Fourier Transform Infrared - methods</topic><topic>Thyroid Gland - chemistry</topic><topic>Thyroid Hormones - analysis</topic><topic>Tissue Array Analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pereira, Thiago M</creatorcontrib><creatorcontrib>Zezell, Denise M</creatorcontrib><creatorcontrib>Bird, Benjamin</creatorcontrib><creatorcontrib>Miljkovi, Milos</creatorcontrib><creatorcontrib>Diem, Max</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pereira, Thiago M</au><au>Zezell, Denise M</au><au>Bird, Benjamin</au><au>Miljkovi, Milos</au><au>Diem, Max</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The characterization of normal thyroid tissue by micro-FTIR spectroscopy</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2013-12-07</date><risdate>2013</risdate><volume>138</volume><issue>23</issue><spage>794</spage><epage>71</epage><pages>794-71</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>In this paper, we report the spectral patterns of normal human thyroid tissue and methodology to interpret hyperspectral imaging data and protein conformational changes observed therein. 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| ispartof | Analyst (London), 2013-12, Vol.138 (23), p.794-71 |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Computer programs Correlation Heterogeneity Humans Matlab Proteins Spectra Spectroscopy, Fourier Transform Infrared - methods Thyroid Gland - chemistry Thyroid Hormones - analysis Tissue Array Analysis |
| title | The characterization of normal thyroid tissue by micro-FTIR spectroscopy |
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