Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA
We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with...
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Veröffentlicht in: | Clinical biochemistry 2013-11, Vol.46 (16-17), p.1717-1722 |
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creator | Ogino, Keiki Murakami, Ikuo Wang, Da-Hong Tsukiyama, Yorika Takahashi, Hidekazu Kubo, Masayuki Sakano, Noriko Setiawan, Heri Bando, Masahiko Ohmoto, Yasukazu |
description | We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with oxidative stress in a larger healthy population by a newly established ELISA.
The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n=721) by comparing a commercial ELISA kit with the new ELISA.
The mean levels of serum arginase I were 20.3±0.7ng/mL and 4.7±0.2ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent.
The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus.
•Serum arginase I is associated with age, serum insulin and urinary 8-OHdG.•Serum arginase I is correlated with WBC, RBC, hs-CRP, HDL-c, ALT and BMI.•New ELISA for arginase I was established compared with a commercial ELISA. |
doi_str_mv | 10.1016/j.clinbiochem.2013.08.012 |
format | Article |
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The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n=721) by comparing a commercial ELISA kit with the new ELISA.
The mean levels of serum arginase I were 20.3±0.7ng/mL and 4.7±0.2ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent.
The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus.
•Serum arginase I is associated with age, serum insulin and urinary 8-OHdG.•Serum arginase I is correlated with WBC, RBC, hs-CRP, HDL-c, ALT and BMI.•New ELISA for arginase I was established compared with a commercial ELISA.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/j.clinbiochem.2013.08.012</identifier><identifier>PMID: 24005081</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>8-Hydroxy-2'-Deoxyguanosine ; Adult ; Animals ; Arginase - blood ; Arginase I ; Asian Continental Ancestry Group ; Biomarkers - blood ; Biomarkers - urine ; Deoxyguanosine - analogs & derivatives ; Deoxyguanosine - urine ; Electrophoresis, Polyacrylamide Gel ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Erythrocytes - metabolism ; Female ; Health ; Humans ; Japan ; Leukocytes - metabolism ; Male ; Oxidative Stress ; Population study ; Rabbits ; Reagent Kits, Diagnostic ; Reference Standards ; Silver Staining ; Statistics, Nonparametric</subject><ispartof>Clinical biochemistry, 2013-11, Vol.46 (16-17), p.1717-1722</ispartof><rights>2013 The Canadian Society of Clinical Chemists</rights><rights>2013.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-e483e8ae4953ea45b0843fb51c94e4713fade35e2b07c24d448580b2f4259e1c3</citedby><cites>FETCH-LOGICAL-c443t-e483e8ae4953ea45b0843fb51c94e4713fade35e2b07c24d448580b2f4259e1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.clinbiochem.2013.08.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24005081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ogino, Keiki</creatorcontrib><creatorcontrib>Murakami, Ikuo</creatorcontrib><creatorcontrib>Wang, Da-Hong</creatorcontrib><creatorcontrib>Tsukiyama, Yorika</creatorcontrib><creatorcontrib>Takahashi, Hidekazu</creatorcontrib><creatorcontrib>Kubo, Masayuki</creatorcontrib><creatorcontrib>Sakano, Noriko</creatorcontrib><creatorcontrib>Setiawan, Heri</creatorcontrib><creatorcontrib>Bando, Masahiko</creatorcontrib><creatorcontrib>Ohmoto, Yasukazu</creatorcontrib><title>Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with oxidative stress in a larger healthy population by a newly established ELISA.
The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n=721) by comparing a commercial ELISA kit with the new ELISA.
The mean levels of serum arginase I were 20.3±0.7ng/mL and 4.7±0.2ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent.
The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus.
•Serum arginase I is associated with age, serum insulin and urinary 8-OHdG.•Serum arginase I is correlated with WBC, RBC, hs-CRP, HDL-c, ALT and BMI.•New ELISA for arginase I was established compared with a commercial ELISA.</description><subject>8-Hydroxy-2'-Deoxyguanosine</subject><subject>Adult</subject><subject>Animals</subject><subject>Arginase - blood</subject><subject>Arginase I</subject><subject>Asian Continental Ancestry Group</subject><subject>Biomarkers - blood</subject><subject>Biomarkers - urine</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - urine</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Erythrocytes - metabolism</subject><subject>Female</subject><subject>Health</subject><subject>Humans</subject><subject>Japan</subject><subject>Leukocytes - metabolism</subject><subject>Male</subject><subject>Oxidative Stress</subject><subject>Population study</subject><subject>Rabbits</subject><subject>Reagent Kits, Diagnostic</subject><subject>Reference Standards</subject><subject>Silver Staining</subject><subject>Statistics, Nonparametric</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu2zAQRYkiReOk_YWA2WUjdfiQRS0Dw2lcGOii7ZqgqFFMl3qUlJx4108vAydFll0NiDl37gwvIdcMcgZs-XmfW-_62g12h13OgYkcVA6MvyMLpkqR8UqIM7IAgCqrGIdzchHjPj25VMsP5JxLgAIUW5A_64Pxs5nc0NOhpRHD3FETHlxvItINNZGa1HlyTWIOSOMUMEaavDsTfmGgrqeG7tD4aXekX81oekzCcRhnf5o6R9c_JKbHR3-kGCdTexd32ND1dvP99iN53xof8dNLvSQ_79Y_VvfZ9tuXzep2m1kpxZShVAKVQVkVAo0salBStHXBbCVRlky0pkFRIK-htFw2UqpCQc1byYsKmRWX5OY0dwzD7zmtoTsXLXqfFh7mqJmUS1WyUrKEVifUhiHGgK0eg0vnHjUD_RyA3us3AejnADQonQJI2qsXm7nusPmnfP3xBKxOAKZjDw6DjtZhb7FxAe2km8H9h81fzmWePA</recordid><startdate>201311</startdate><enddate>201311</enddate><creator>Ogino, Keiki</creator><creator>Murakami, Ikuo</creator><creator>Wang, Da-Hong</creator><creator>Tsukiyama, Yorika</creator><creator>Takahashi, Hidekazu</creator><creator>Kubo, Masayuki</creator><creator>Sakano, Noriko</creator><creator>Setiawan, Heri</creator><creator>Bando, Masahiko</creator><creator>Ohmoto, Yasukazu</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201311</creationdate><title>Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA</title><author>Ogino, Keiki ; Murakami, Ikuo ; Wang, Da-Hong ; Tsukiyama, Yorika ; Takahashi, Hidekazu ; Kubo, Masayuki ; Sakano, Noriko ; Setiawan, Heri ; Bando, Masahiko ; Ohmoto, Yasukazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-e483e8ae4953ea45b0843fb51c94e4713fade35e2b07c24d448580b2f4259e1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>8-Hydroxy-2'-Deoxyguanosine</topic><topic>Adult</topic><topic>Animals</topic><topic>Arginase - blood</topic><topic>Arginase I</topic><topic>Asian Continental Ancestry Group</topic><topic>Biomarkers - blood</topic><topic>Biomarkers - urine</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - urine</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Erythrocytes - metabolism</topic><topic>Female</topic><topic>Health</topic><topic>Humans</topic><topic>Japan</topic><topic>Leukocytes - metabolism</topic><topic>Male</topic><topic>Oxidative Stress</topic><topic>Population study</topic><topic>Rabbits</topic><topic>Reagent Kits, Diagnostic</topic><topic>Reference Standards</topic><topic>Silver Staining</topic><topic>Statistics, Nonparametric</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ogino, Keiki</creatorcontrib><creatorcontrib>Murakami, Ikuo</creatorcontrib><creatorcontrib>Wang, Da-Hong</creatorcontrib><creatorcontrib>Tsukiyama, Yorika</creatorcontrib><creatorcontrib>Takahashi, Hidekazu</creatorcontrib><creatorcontrib>Kubo, Masayuki</creatorcontrib><creatorcontrib>Sakano, Noriko</creatorcontrib><creatorcontrib>Setiawan, Heri</creatorcontrib><creatorcontrib>Bando, Masahiko</creatorcontrib><creatorcontrib>Ohmoto, Yasukazu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogino, Keiki</au><au>Murakami, Ikuo</au><au>Wang, Da-Hong</au><au>Tsukiyama, Yorika</au><au>Takahashi, Hidekazu</au><au>Kubo, Masayuki</au><au>Sakano, Noriko</au><au>Setiawan, Heri</au><au>Bando, Masahiko</au><au>Ohmoto, Yasukazu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2013-11</date><risdate>2013</risdate><volume>46</volume><issue>16-17</issue><spage>1717</spage><epage>1722</epage><pages>1717-1722</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with oxidative stress in a larger healthy population by a newly established ELISA.
The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n=721) by comparing a commercial ELISA kit with the new ELISA.
The mean levels of serum arginase I were 20.3±0.7ng/mL and 4.7±0.2ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent.
The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus.
•Serum arginase I is associated with age, serum insulin and urinary 8-OHdG.•Serum arginase I is correlated with WBC, RBC, hs-CRP, HDL-c, ALT and BMI.•New ELISA for arginase I was established compared with a commercial ELISA.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24005081</pmid><doi>10.1016/j.clinbiochem.2013.08.012</doi><tpages>6</tpages></addata></record> |
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subjects | 8-Hydroxy-2'-Deoxyguanosine Adult Animals Arginase - blood Arginase I Asian Continental Ancestry Group Biomarkers - blood Biomarkers - urine Deoxyguanosine - analogs & derivatives Deoxyguanosine - urine Electrophoresis, Polyacrylamide Gel ELISA Enzyme-Linked Immunosorbent Assay - methods Erythrocytes - metabolism Female Health Humans Japan Leukocytes - metabolism Male Oxidative Stress Population study Rabbits Reagent Kits, Diagnostic Reference Standards Silver Staining Statistics, Nonparametric |
title | Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA |
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