Accumulation of PrLeg, a perilla legumin protein in potato tuber results in enhanced level of sulphur-containing amino acids
Potato is the fourth staple food in the world, following rice, wheat, and maize, whereas tubers contain high quality of starch, relatively high amounts of vitamin C and many other important substances. It also contains relatively good quality of protein (about 3 to 6% of the dried weight) and patati...
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description | Potato is the fourth staple food in the world, following rice, wheat, and maize, whereas tubers contain high quality of starch, relatively high amounts of vitamin C and many other important substances. It also contains relatively good quality of protein (about 3 to 6% of the dried weight) and patatin, and 11S globulin is a major storage protein with high level of lysine. However, tuber protein contains relatively low amounts of sulphur-containing amino acids, which may result in low nutritional value. Recently, we cloned a gene encoding PrLeg polypeptide, a seed storage protein from perilla, which contains relatively higher levels of sulphur-containing amino acids. We transformed PrLeg cDNA into a potato plant to over-express under the direction of the tuber-specific promoter, patatin. Most of the transgenic lines identified through PCR and RT–PCR analyses were able to accumulate high amount of prLeg transcript in their tuber tissue, while very little or no transcript that were detected in their leaf tissues. The level of methionine content was elevated up to three-fold compared to non-transgenic parental line, without any significant changes in other amino acids, suggesting that further research is required to get a deeper insight into their nutritional value. |
doi_str_mv | 10.1016/j.crvi.2013.09.002 |
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It also contains relatively good quality of protein (about 3 to 6% of the dried weight) and patatin, and 11S globulin is a major storage protein with high level of lysine. However, tuber protein contains relatively low amounts of sulphur-containing amino acids, which may result in low nutritional value. Recently, we cloned a gene encoding PrLeg polypeptide, a seed storage protein from perilla, which contains relatively higher levels of sulphur-containing amino acids. We transformed PrLeg cDNA into a potato plant to over-express under the direction of the tuber-specific promoter, patatin. Most of the transgenic lines identified through PCR and RT–PCR analyses were able to accumulate high amount of prLeg transcript in their tuber tissue, while very little or no transcript that were detected in their leaf tissues. The level of methionine content was elevated up to three-fold compared to non-transgenic parental line, without any significant changes in other amino acids, suggesting that further research is required to get a deeper insight into their nutritional value.</description><identifier>ISSN: 1631-0691</identifier><identifier>ISSN: 1768-3238</identifier><identifier>EISSN: 1768-3238</identifier><identifier>DOI: 10.1016/j.crvi.2013.09.002</identifier><identifier>PMID: 24161240</identifier><language>eng</language><publisher>France: Elsevier SAS</publisher><subject>Amino Acids - analysis ; ascorbic acid ; Carboxylic Ester Hydrolases - genetics ; complementary DNA ; corn ; DNA, Complementary - genetics ; Gene Expression Regulation, Plant ; genes ; Genetic Enhancement ; genetically modified organisms ; leaves ; legumin ; Legumins ; lysine ; Methionine ; Methionine - analysis ; Methionine - metabolism ; nutritive value ; Patatin ; Perilla ; Perilla frutescens - genetics ; Plant Leaves - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Tubers - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; polypeptides ; Potato ; potato protein ; potatoes ; PrLeg ; Promoter Regions, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; rice ; RNA, Messenger - biosynthesis ; RNA, Plant - biosynthesis ; seed storage ; Solanum tuberosum - genetics ; Solanum tuberosum - metabolism ; starch ; Sulphur-containing amino acid ; tissues ; Transgenes ; tubers ; wheat</subject><ispartof>Comptes rendus. Biologies, 2013-09, Vol.336 (9), p.433-439</ispartof><rights>2013 Académie des sciences</rights><rights>Copyright © 2013 Académie des sciences. Published by Elsevier SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-92e145a7e7e3756425620a0401bb6f16ab4f4d084d2a87531e1264ecbd50a8bc3</citedby><cites>FETCH-LOGICAL-c380t-92e145a7e7e3756425620a0401bb6f16ab4f4d084d2a87531e1264ecbd50a8bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.crvi.2013.09.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24161240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goo, Young-Min</creatorcontrib><creatorcontrib>Kim, Tae-Won</creatorcontrib><creatorcontrib>Lee, Min-Kyung</creatorcontrib><creatorcontrib>Lee, Shin-Woo</creatorcontrib><title>Accumulation of PrLeg, a perilla legumin protein in potato tuber results in enhanced level of sulphur-containing amino acids</title><title>Comptes rendus. Biologies</title><addtitle>C R Biol</addtitle><description>Potato is the fourth staple food in the world, following rice, wheat, and maize, whereas tubers contain high quality of starch, relatively high amounts of vitamin C and many other important substances. It also contains relatively good quality of protein (about 3 to 6% of the dried weight) and patatin, and 11S globulin is a major storage protein with high level of lysine. However, tuber protein contains relatively low amounts of sulphur-containing amino acids, which may result in low nutritional value. Recently, we cloned a gene encoding PrLeg polypeptide, a seed storage protein from perilla, which contains relatively higher levels of sulphur-containing amino acids. We transformed PrLeg cDNA into a potato plant to over-express under the direction of the tuber-specific promoter, patatin. Most of the transgenic lines identified through PCR and RT–PCR analyses were able to accumulate high amount of prLeg transcript in their tuber tissue, while very little or no transcript that were detected in their leaf tissues. The level of methionine content was elevated up to three-fold compared to non-transgenic parental line, without any significant changes in other amino acids, suggesting that further research is required to get a deeper insight into their nutritional value.</description><subject>Amino Acids - analysis</subject><subject>ascorbic acid</subject><subject>Carboxylic Ester Hydrolases - genetics</subject><subject>complementary DNA</subject><subject>corn</subject><subject>DNA, Complementary - genetics</subject><subject>Gene Expression Regulation, Plant</subject><subject>genes</subject><subject>Genetic Enhancement</subject><subject>genetically modified organisms</subject><subject>leaves</subject><subject>legumin</subject><subject>Legumins</subject><subject>lysine</subject><subject>Methionine</subject><subject>Methionine - analysis</subject><subject>Methionine - metabolism</subject><subject>nutritive value</subject><subject>Patatin</subject><subject>Perilla</subject><subject>Perilla frutescens - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Tubers - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>polypeptides</subject><subject>Potato</subject><subject>potato protein</subject><subject>potatoes</subject><subject>PrLeg</subject><subject>Promoter Regions, Genetic</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>rice</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Plant - biosynthesis</subject><subject>seed storage</subject><subject>Solanum tuberosum - genetics</subject><subject>Solanum tuberosum - metabolism</subject><subject>starch</subject><subject>Sulphur-containing amino acid</subject><subject>tissues</subject><subject>Transgenes</subject><subject>tubers</subject><subject>wheat</subject><issn>1631-0691</issn><issn>1768-3238</issn><issn>1768-3238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2L1TAUhoMozof-AReapYtpPUnTNAU3w-AXXFDQWYc0Pb2TS9tc83FB8MebckeXrnJInvcl5yHkFYOaAZPvDrUNJ1dzYE0NfQ3An5BL1klVNbxRT8ssG1aB7NkFuYrxACXUt-1zcsEFk4wLuCS_b63NS55Ncn6lfqLfwg73N9TQIwY3z4bOuM-LW-kx-ITl3EafTPI05QEDDRjznOJ2j-uDWS2OJXPCeWsrT8eHHCrr12Tc6tY9NaXMU2PdGF-QZ5OZI758PK_J_ccPP-4-V7uvn77c3e4q2yhIVc-RidZ02GHTtVLwVnIwIIANg5yYNIOYxAhKjNyorm0YMi4F2mFswajBNtfk7bm37PAzY0x6cdFi2W5Fn6NmQkjVMd5DQfkZtcHHGHDSx-AWE35pBnqzrg96s6436xp6XayX0OvH_jwsOP6L_NVcgDdnYDJem31wUd9_Lw0SAFQhVCHenwksHk4Og47W4SbTBbRJj9797wd_APyCnag</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Goo, Young-Min</creator><creator>Kim, Tae-Won</creator><creator>Lee, Min-Kyung</creator><creator>Lee, Shin-Woo</creator><general>Elsevier SAS</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130901</creationdate><title>Accumulation of PrLeg, a perilla legumin protein in potato tuber results in enhanced level of sulphur-containing amino acids</title><author>Goo, Young-Min ; Kim, Tae-Won ; Lee, Min-Kyung ; Lee, Shin-Woo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-92e145a7e7e3756425620a0401bb6f16ab4f4d084d2a87531e1264ecbd50a8bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino Acids - analysis</topic><topic>ascorbic acid</topic><topic>Carboxylic Ester Hydrolases - genetics</topic><topic>complementary DNA</topic><topic>corn</topic><topic>DNA, Complementary - genetics</topic><topic>Gene Expression Regulation, Plant</topic><topic>genes</topic><topic>Genetic Enhancement</topic><topic>genetically modified organisms</topic><topic>leaves</topic><topic>legumin</topic><topic>Legumins</topic><topic>lysine</topic><topic>Methionine</topic><topic>Methionine - analysis</topic><topic>Methionine - metabolism</topic><topic>nutritive value</topic><topic>Patatin</topic><topic>Perilla</topic><topic>Perilla frutescens - genetics</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Tubers - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>polypeptides</topic><topic>Potato</topic><topic>potato protein</topic><topic>potatoes</topic><topic>PrLeg</topic><topic>Promoter Regions, Genetic</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>rice</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Plant - biosynthesis</topic><topic>seed storage</topic><topic>Solanum tuberosum - genetics</topic><topic>Solanum tuberosum - metabolism</topic><topic>starch</topic><topic>Sulphur-containing amino acid</topic><topic>tissues</topic><topic>Transgenes</topic><topic>tubers</topic><topic>wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goo, Young-Min</creatorcontrib><creatorcontrib>Kim, Tae-Won</creatorcontrib><creatorcontrib>Lee, Min-Kyung</creatorcontrib><creatorcontrib>Lee, Shin-Woo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Comptes rendus. Biologies</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goo, Young-Min</au><au>Kim, Tae-Won</au><au>Lee, Min-Kyung</au><au>Lee, Shin-Woo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accumulation of PrLeg, a perilla legumin protein in potato tuber results in enhanced level of sulphur-containing amino acids</atitle><jtitle>Comptes rendus. Biologies</jtitle><addtitle>C R Biol</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>336</volume><issue>9</issue><spage>433</spage><epage>439</epage><pages>433-439</pages><issn>1631-0691</issn><issn>1768-3238</issn><eissn>1768-3238</eissn><abstract>Potato is the fourth staple food in the world, following rice, wheat, and maize, whereas tubers contain high quality of starch, relatively high amounts of vitamin C and many other important substances. It also contains relatively good quality of protein (about 3 to 6% of the dried weight) and patatin, and 11S globulin is a major storage protein with high level of lysine. However, tuber protein contains relatively low amounts of sulphur-containing amino acids, which may result in low nutritional value. Recently, we cloned a gene encoding PrLeg polypeptide, a seed storage protein from perilla, which contains relatively higher levels of sulphur-containing amino acids. We transformed PrLeg cDNA into a potato plant to over-express under the direction of the tuber-specific promoter, patatin. Most of the transgenic lines identified through PCR and RT–PCR analyses were able to accumulate high amount of prLeg transcript in their tuber tissue, while very little or no transcript that were detected in their leaf tissues. The level of methionine content was elevated up to three-fold compared to non-transgenic parental line, without any significant changes in other amino acids, suggesting that further research is required to get a deeper insight into their nutritional value.</abstract><cop>France</cop><pub>Elsevier SAS</pub><pmid>24161240</pmid><doi>10.1016/j.crvi.2013.09.002</doi><tpages>7</tpages></addata></record> |
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subjects | Amino Acids - analysis ascorbic acid Carboxylic Ester Hydrolases - genetics complementary DNA corn DNA, Complementary - genetics Gene Expression Regulation, Plant genes Genetic Enhancement genetically modified organisms leaves legumin Legumins lysine Methionine Methionine - analysis Methionine - metabolism nutritive value Patatin Perilla Perilla frutescens - genetics Plant Leaves - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plant Tubers - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism polypeptides Potato potato protein potatoes PrLeg Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction rice RNA, Messenger - biosynthesis RNA, Plant - biosynthesis seed storage Solanum tuberosum - genetics Solanum tuberosum - metabolism starch Sulphur-containing amino acid tissues Transgenes tubers wheat |
title | Accumulation of PrLeg, a perilla legumin protein in potato tuber results in enhanced level of sulphur-containing amino acids |
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