Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7
Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2013-08, Vol.159 (Pt 8), p.1629-1638 |
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| creator | Shahrbabak, Salehe Sabouri Khodabandehlou, Zahra Shahverdi, Ahmad Reza Skurnik, Mikael Ackermann, Hans-Wolfgang Varjosalo, Markku Yazdi, Mojtaba Tabatabaei Sepehrizadeh, Zargham |
| description | Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115×15 nm. PhaxI contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7. |
| doi_str_mv | 10.1099/mic.0.063776-0 |
| format | Article |
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Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115×15 nm. PhaxI contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/mic.0.063776-0</identifier><identifier>PMID: 23676434</identifier><language>eng</language><publisher>England</publisher><subject>Base Composition ; Coliphages - chemistry ; Coliphages - genetics ; Coliphages - isolation & purification ; Coliphages - ultrastructure ; DNA, Viral - chemistry ; DNA, Viral - genetics ; Escherichia coli O157 - virology ; Genome, Viral ; Iran ; Mass Spectrometry ; Microscopy, Electron, Transmission ; Molecular Sequence Data ; Myoviridae - chemistry ; Myoviridae - genetics ; Myoviridae - isolation & purification ; Myoviridae - ultrastructure ; Open Reading Frames ; Sequence Analysis, DNA ; Sewage - virology ; Virion - chemistry ; Virion - ultrastructure</subject><ispartof>Microbiology (Society for General Microbiology), 2013-08, Vol.159 (Pt 8), p.1629-1638</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c210t-9cb3959102a6607b4f721c524951bb438bd9bba99fae71045e7e9ffa6f87dc553</citedby><cites>FETCH-LOGICAL-c210t-9cb3959102a6607b4f721c524951bb438bd9bba99fae71045e7e9ffa6f87dc553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23676434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shahrbabak, Salehe Sabouri</creatorcontrib><creatorcontrib>Khodabandehlou, Zahra</creatorcontrib><creatorcontrib>Shahverdi, Ahmad Reza</creatorcontrib><creatorcontrib>Skurnik, Mikael</creatorcontrib><creatorcontrib>Ackermann, Hans-Wolfgang</creatorcontrib><creatorcontrib>Varjosalo, Markku</creatorcontrib><creatorcontrib>Yazdi, Mojtaba Tabatabaei</creatorcontrib><creatorcontrib>Sepehrizadeh, Zargham</creatorcontrib><title>Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115×15 nm. PhaxI contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.</description><subject>Base Composition</subject><subject>Coliphages - chemistry</subject><subject>Coliphages - genetics</subject><subject>Coliphages - isolation & purification</subject><subject>Coliphages - ultrastructure</subject><subject>DNA, Viral - chemistry</subject><subject>DNA, Viral - genetics</subject><subject>Escherichia coli O157 - virology</subject><subject>Genome, Viral</subject><subject>Iran</subject><subject>Mass Spectrometry</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular Sequence Data</subject><subject>Myoviridae - chemistry</subject><subject>Myoviridae - genetics</subject><subject>Myoviridae - isolation & purification</subject><subject>Myoviridae - ultrastructure</subject><subject>Open Reading Frames</subject><subject>Sequence Analysis, DNA</subject><subject>Sewage - virology</subject><subject>Virion - chemistry</subject><subject>Virion - ultrastructure</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kL1PwzAQxS0EolBYGZFHBhLO8VfcDVWFVqpUBpgjx3GaoCQOcSoBfz2mLUx3evd-T6eH0A2BmIBSD21tYohBUClFBCfogjDBowRSOA075RBBKpMJuvT-HSAcgZyjSUKFFIyyC1StvGv0WLvuHptKD9qMdqi_9wrWXYGNa_vGjhZvbedai7392NnOWOxK_FLpz9UMa9xXertXFt5UgTdVrQPZ1HhDuMQzvJRX6KzUjbfXxzlFb0-L1_kyWm-eV_PHdWQSAmOkTE4VVwQSLQTInJUyIYYnTHGS54ymeaHyXCtVaisJMG6lVWWpRZnKwnBOp-jukNsPLnzqx6ytvbFNozvrdj4jjIV4QZUK1vhgNYPzfrBl1g91q4evjED2225ATQbZod0MAnB7zN7lrS3-7X910h8OP3SH</recordid><startdate>201308</startdate><enddate>201308</enddate><creator>Shahrbabak, Salehe Sabouri</creator><creator>Khodabandehlou, Zahra</creator><creator>Shahverdi, Ahmad Reza</creator><creator>Skurnik, Mikael</creator><creator>Ackermann, Hans-Wolfgang</creator><creator>Varjosalo, Markku</creator><creator>Yazdi, Mojtaba Tabatabaei</creator><creator>Sepehrizadeh, Zargham</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201308</creationdate><title>Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7</title><author>Shahrbabak, Salehe Sabouri ; 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Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115×15 nm. PhaxI contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.</abstract><cop>England</cop><pmid>23676434</pmid><doi>10.1099/mic.0.063776-0</doi><tpages>10</tpages></addata></record> |
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| subjects | Base Composition Coliphages - chemistry Coliphages - genetics Coliphages - isolation & purification Coliphages - ultrastructure DNA, Viral - chemistry DNA, Viral - genetics Escherichia coli O157 - virology Genome, Viral Iran Mass Spectrometry Microscopy, Electron, Transmission Molecular Sequence Data Myoviridae - chemistry Myoviridae - genetics Myoviridae - isolation & purification Myoviridae - ultrastructure Open Reading Frames Sequence Analysis, DNA Sewage - virology Virion - chemistry Virion - ultrastructure |
| title | Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7 |
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