Medium optimization, molecular characterization, and bioactivity of exopolysaccharides from Pleurotus eryngii
An optimal medium for exopolysaccharides (EPS) production was obtained through one-factor-at-a-time method and response surface methodology. Under optimal culture medium, the maximum EPS concentration in shake flask was 5.16 g/l. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from t...
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| Veröffentlicht in: | Archives of microbiology 2013-11, Vol.195 (10-11), p.749-757 |
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| creator | Jing, Xiaoyan Mao, Duobin Geng, Lujing Xu, Chunping |
| description | An optimal medium for exopolysaccharides (EPS) production was obtained through one-factor-at-a-time method and response surface methodology. Under optimal culture medium, the maximum EPS concentration in shake flask was 5.16 g/l. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography/multiangle laser light scattering, and the weight average molar masses (Mw) of Fr-I and Fr-II were determined to be 4.098 × 10⁴and 1.114 × 10⁴ g/mol, respectively. The molecular confirmation of Fr-I was revealed to be a rigid rod form in aqueous solution. Moreover, monosaccharide composition and characteristic groups were investigated by GC and Fourier transform infrared, respectively. Finally, pharmacology experiment in vitro indicated EPS Fr-II of Pleurotus eryngii exhibited higher antioxidant and antitumor abilities than Fr-I, which might be attributed to the different molecular weights and chemical compositions in the EPS fraction. |
| doi_str_mv | 10.1007/s00203-013-0927-1 |
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Under optimal culture medium, the maximum EPS concentration in shake flask was 5.16 g/l. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography/multiangle laser light scattering, and the weight average molar masses (Mw) of Fr-I and Fr-II were determined to be 4.098 × 10⁴and 1.114 × 10⁴ g/mol, respectively. The molecular confirmation of Fr-I was revealed to be a rigid rod form in aqueous solution. Moreover, monosaccharide composition and characteristic groups were investigated by GC and Fourier transform infrared, respectively. Finally, pharmacology experiment in vitro indicated EPS Fr-II of Pleurotus eryngii exhibited higher antioxidant and antitumor abilities than Fr-I, which might be attributed to the different molecular weights and chemical compositions in the EPS fraction.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-013-0927-1</identifier><identifier>PMID: 24071733</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Antineoplastic Agents - pharmacology ; Antioxidants ; Antioxidants - pharmacology ; aqueous solutions ; Biochemistry ; Biological activity ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Chromatography ; culture filtrates ; culture media ; Culture Media - chemistry ; Ecology ; exopolysaccharides ; Fourier transforms ; Fungal Polysaccharides - biosynthesis ; Fungal Polysaccharides - chemistry ; Fungal Polysaccharides - isolation & purification ; Fungal Polysaccharides - pharmacology ; Fungi ; gel chromatography ; in vitro studies ; Life Sciences ; Light scattering ; Microbial Ecology ; Microbiology ; Molecular Weight ; Optimization ; Original Paper ; Pharmacology ; Physiology ; Pleurotus - chemistry ; Pleurotus - metabolism ; Pleurotus eryngii ; response surface methodology ; Spectroscopy, Fourier Transform Infrared</subject><ispartof>Archives of microbiology, 2013-11, Vol.195 (10-11), p.749-757</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-8a567a70450910f32ffb18452d7bec20ba9ca9f30c4cf0f4acc9068382f4c6d93</citedby><cites>FETCH-LOGICAL-c396t-8a567a70450910f32ffb18452d7bec20ba9ca9f30c4cf0f4acc9068382f4c6d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-013-0927-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-013-0927-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24071733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jing, Xiaoyan</creatorcontrib><creatorcontrib>Mao, Duobin</creatorcontrib><creatorcontrib>Geng, Lujing</creatorcontrib><creatorcontrib>Xu, Chunping</creatorcontrib><title>Medium optimization, molecular characterization, and bioactivity of exopolysaccharides from Pleurotus eryngii</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>An optimal medium for exopolysaccharides (EPS) production was obtained through one-factor-at-a-time method and response surface methodology. Under optimal culture medium, the maximum EPS concentration in shake flask was 5.16 g/l. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography/multiangle laser light scattering, and the weight average molar masses (Mw) of Fr-I and Fr-II were determined to be 4.098 × 10⁴and 1.114 × 10⁴ g/mol, respectively. The molecular confirmation of Fr-I was revealed to be a rigid rod form in aqueous solution. Moreover, monosaccharide composition and characteristic groups were investigated by GC and Fourier transform infrared, respectively. Finally, pharmacology experiment in vitro indicated EPS Fr-II of Pleurotus eryngii exhibited higher antioxidant and antitumor abilities than Fr-I, which might be attributed to the different molecular weights and chemical compositions in the EPS fraction.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>aqueous solutions</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Chromatography</subject><subject>culture filtrates</subject><subject>culture media</subject><subject>Culture Media - chemistry</subject><subject>Ecology</subject><subject>exopolysaccharides</subject><subject>Fourier transforms</subject><subject>Fungal Polysaccharides - biosynthesis</subject><subject>Fungal Polysaccharides - chemistry</subject><subject>Fungal Polysaccharides - isolation & purification</subject><subject>Fungal Polysaccharides - pharmacology</subject><subject>Fungi</subject><subject>gel chromatography</subject><subject>in vitro studies</subject><subject>Life Sciences</subject><subject>Light scattering</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Optimization</subject><subject>Original Paper</subject><subject>Pharmacology</subject><subject>Physiology</subject><subject>Pleurotus - chemistry</subject><subject>Pleurotus - metabolism</subject><subject>Pleurotus eryngii</subject><subject>response surface methodology</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1P3DAQhq0KVBbaH9ALWOqFA2nHHxvHR7QqHxKISi1Sb5bj2FujJF7spGL59TgKRYgDB2skz_O-M5oXoS8EvhEA8T0BUGAFkPwkFQX5gBaEM1qAoH920AIY0KKSjO2h_ZTuAAitquoj2qMcBBGMLVB3bRs_djhsBt_5Rz340J_gLrTWjK2O2PzVUZvBxpee7htc-5A__T8_bHFw2D6ETWi3SZsJ941N2MXQ4Z-tHWMYxoRt3PZr7z-hXafbZD8_1wN0e_bj9-qiuLo5v1ydXhWGyXIoKr0shRbAlyAJOEadq0nFl7QRtTUUai2Nlo6B4caB43muhLJiFXXclI1kB-h49t3EcD_aNKjOJ2PbVvc2jEkRzpmUZUl5Rr--Qe_CGPu83UTRfFbgkyGZKRNDStE6tYm-03GrCKgpCzVnoXIWaspCkaw5fHYe6842L4r_x88AnYGUW_3axlej33E9mkVOB6XX0Sd1-4sCWWaSMCGBPQHlop8a</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Jing, Xiaoyan</creator><creator>Mao, Duobin</creator><creator>Geng, Lujing</creator><creator>Xu, Chunping</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20131101</creationdate><title>Medium optimization, molecular characterization, and bioactivity of exopolysaccharides from Pleurotus eryngii</title><author>Jing, Xiaoyan ; Mao, Duobin ; Geng, Lujing ; Xu, Chunping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-8a567a70450910f32ffb18452d7bec20ba9ca9f30c4cf0f4acc9068382f4c6d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antineoplastic Agents - 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Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jing, Xiaoyan</au><au>Mao, Duobin</au><au>Geng, Lujing</au><au>Xu, Chunping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Medium optimization, molecular characterization, and bioactivity of exopolysaccharides from Pleurotus eryngii</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>195</volume><issue>10-11</issue><spage>749</spage><epage>757</epage><pages>749-757</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>An optimal medium for exopolysaccharides (EPS) production was obtained through one-factor-at-a-time method and response surface methodology. Under optimal culture medium, the maximum EPS concentration in shake flask was 5.16 g/l. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography/multiangle laser light scattering, and the weight average molar masses (Mw) of Fr-I and Fr-II were determined to be 4.098 × 10⁴and 1.114 × 10⁴ g/mol, respectively. The molecular confirmation of Fr-I was revealed to be a rigid rod form in aqueous solution. Moreover, monosaccharide composition and characteristic groups were investigated by GC and Fourier transform infrared, respectively. Finally, pharmacology experiment in vitro indicated EPS Fr-II of Pleurotus eryngii exhibited higher antioxidant and antitumor abilities than Fr-I, which might be attributed to the different molecular weights and chemical compositions in the EPS fraction.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>24071733</pmid><doi>10.1007/s00203-013-0927-1</doi><tpages>9</tpages></addata></record> |
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| subjects | Antineoplastic Agents - pharmacology Antioxidants Antioxidants - pharmacology aqueous solutions Biochemistry Biological activity Biomedical and Life Sciences Biotechnology Cell Biology Chromatography culture filtrates culture media Culture Media - chemistry Ecology exopolysaccharides Fourier transforms Fungal Polysaccharides - biosynthesis Fungal Polysaccharides - chemistry Fungal Polysaccharides - isolation & purification Fungal Polysaccharides - pharmacology Fungi gel chromatography in vitro studies Life Sciences Light scattering Microbial Ecology Microbiology Molecular Weight Optimization Original Paper Pharmacology Physiology Pleurotus - chemistry Pleurotus - metabolism Pleurotus eryngii response surface methodology Spectroscopy, Fourier Transform Infrared |
| title | Medium optimization, molecular characterization, and bioactivity of exopolysaccharides from Pleurotus eryngii |
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