Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression
Abstract Introduction Alterations of apoptosis are commonly associated with pregnancy complications and abortion. Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors...
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| Veröffentlicht in: | Placenta (Eastbourne) 2013-11, Vol.34 (11), p.973-982 |
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| creator | Angeloni, M.B Guirelli, P.M Franco, P.S Barbosa, B.F Gomes, A.O Castro, A.S Silva, N.M Martins-Filho, O.A Mineo, T.W.P Silva, D.A.O Mineo, J.R Ferro, E.A.V |
| description | Abstract Introduction Alterations of apoptosis are commonly associated with pregnancy complications and abortion. Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors that are involved in the differential apoptosis of BeWo cells infected with distinct T. gondii strain types. Methods Human trophoblastic cells (BeWo cell line) were infected with RH or ME49 strains, the cytokine production was measured and the phosphorylation of anti-apoptotic ERK1/2 protein was analyzed. Also, cells were treated with different cytokines, infected with RH or ME49 strain, and analyzed for apoptosis index and Fas/CD95 death receptor expression. Results ME49-infected BeWo cells exhibited a predominantly pro-inflammatory cytokine profile, whereas cells infected with RH strain had a higher production of anti-inflammatory cytokines. Also, the incidence of apoptosis was higher in ME49-infected cells, which have been treated with pro-inflammatory cytokines compared to cells infected with RH and treated with anti-inflammatory cytokines. Moreover, Fas/CD95 expression was higher in cells infected with either ME49 or RH strain and treated with pro-inflammatory cytokines compared to anti-inflammatory cytokine treatment. The phosphorylation of ERK1/2 protein increased after 24 h of infection only with the RH strain. Conclusion These results suggest that opposing mechanisms of interference in apoptosis of BeWo cells after infection with RH or ME49 strains of T. gondii can be associated with the differential cytokine profile secreted, the Fas/CD95 expression and the phosphorylated ERK1/2 expression. |
| doi_str_mv | 10.1016/j.placenta.2013.09.005 |
| format | Article |
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Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors that are involved in the differential apoptosis of BeWo cells infected with distinct T. gondii strain types. Methods Human trophoblastic cells (BeWo cell line) were infected with RH or ME49 strains, the cytokine production was measured and the phosphorylation of anti-apoptotic ERK1/2 protein was analyzed. Also, cells were treated with different cytokines, infected with RH or ME49 strain, and analyzed for apoptosis index and Fas/CD95 death receptor expression. Results ME49-infected BeWo cells exhibited a predominantly pro-inflammatory cytokine profile, whereas cells infected with RH strain had a higher production of anti-inflammatory cytokines. Also, the incidence of apoptosis was higher in ME49-infected cells, which have been treated with pro-inflammatory cytokines compared to cells infected with RH and treated with anti-inflammatory cytokines. Moreover, Fas/CD95 expression was higher in cells infected with either ME49 or RH strain and treated with pro-inflammatory cytokines compared to anti-inflammatory cytokine treatment. The phosphorylation of ERK1/2 protein increased after 24 h of infection only with the RH strain. Conclusion These results suggest that opposing mechanisms of interference in apoptosis of BeWo cells after infection with RH or ME49 strains of T. gondii can be associated with the differential cytokine profile secreted, the Fas/CD95 expression and the phosphorylated ERK1/2 expression.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/j.placenta.2013.09.005</identifier><identifier>PMID: 24074900</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Apoptosis ; BeWo cells ; Biological and medical sciences ; Cell Line ; Congenital toxoplasmosis ; Cytokines - genetics ; Cytokines - metabolism ; Cytokines - secretion ; Embryology: invertebrates and vertebrates. Teratology ; fas Receptor - biosynthesis ; fas Receptor - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Internal Medicine ; Mitogen-Activated Protein Kinase 1 - biosynthesis ; Mitogen-Activated Protein Kinase 1 - metabolism ; Mitogen-Activated Protein Kinase 3 - biosynthesis ; Mitogen-Activated Protein Kinase 3 - metabolism ; Obstetrics and Gynecology ; Phosphorylation ; Placenta - immunology ; Placenta - metabolism ; Placenta - parasitology ; Placenta - secretion ; Placentation ; Pregnancy ; Pregnancy Complications, Parasitic - immunology ; Pregnancy Complications, Parasitic - metabolism ; Pregnancy Complications, Parasitic - parasitology ; Pregnancy Complications, Parasitic - pathology ; Protein Processing, Post-Translational ; Recombinant Proteins - metabolism ; Species Specificity ; Toxoplasma - immunology ; Toxoplasma - pathogenicity ; Toxoplasma gondii ; Toxoplasmosis - immunology ; Toxoplasmosis - metabolism ; Toxoplasmosis - parasitology ; Toxoplasmosis - pathology ; Trophoblastic cells ; Trophoblasts - immunology ; Trophoblasts - metabolism ; Trophoblasts - parasitology ; Trophoblasts - secretion ; Up-Regulation ; Virulence</subject><ispartof>Placenta (Eastbourne), 2013-11, Vol.34 (11), p.973-982</ispartof><rights>Elsevier Ltd</rights><rights>2013 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-c7031761d84f3004c6bba6bd54a7929ba9fca3a35d27c2b933d27635436754963</citedby><cites>FETCH-LOGICAL-c501t-c7031761d84f3004c6bba6bd54a7929ba9fca3a35d27c2b933d27635436754963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0143400413007315$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27918519$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24074900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Angeloni, M.B</creatorcontrib><creatorcontrib>Guirelli, P.M</creatorcontrib><creatorcontrib>Franco, P.S</creatorcontrib><creatorcontrib>Barbosa, B.F</creatorcontrib><creatorcontrib>Gomes, A.O</creatorcontrib><creatorcontrib>Castro, A.S</creatorcontrib><creatorcontrib>Silva, N.M</creatorcontrib><creatorcontrib>Martins-Filho, O.A</creatorcontrib><creatorcontrib>Mineo, T.W.P</creatorcontrib><creatorcontrib>Silva, D.A.O</creatorcontrib><creatorcontrib>Mineo, J.R</creatorcontrib><creatorcontrib>Ferro, E.A.V</creatorcontrib><title>Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Abstract Introduction Alterations of apoptosis are commonly associated with pregnancy complications and abortion. Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors that are involved in the differential apoptosis of BeWo cells infected with distinct T. gondii strain types. Methods Human trophoblastic cells (BeWo cell line) were infected with RH or ME49 strains, the cytokine production was measured and the phosphorylation of anti-apoptotic ERK1/2 protein was analyzed. Also, cells were treated with different cytokines, infected with RH or ME49 strain, and analyzed for apoptosis index and Fas/CD95 death receptor expression. Results ME49-infected BeWo cells exhibited a predominantly pro-inflammatory cytokine profile, whereas cells infected with RH strain had a higher production of anti-inflammatory cytokines. Also, the incidence of apoptosis was higher in ME49-infected cells, which have been treated with pro-inflammatory cytokines compared to cells infected with RH and treated with anti-inflammatory cytokines. Moreover, Fas/CD95 expression was higher in cells infected with either ME49 or RH strain and treated with pro-inflammatory cytokines compared to anti-inflammatory cytokine treatment. The phosphorylation of ERK1/2 protein increased after 24 h of infection only with the RH strain. Conclusion These results suggest that opposing mechanisms of interference in apoptosis of BeWo cells after infection with RH or ME49 strains of T. gondii can be associated with the differential cytokine profile secreted, the Fas/CD95 expression and the phosphorylated ERK1/2 expression.</description><subject>Apoptosis</subject><subject>BeWo cells</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Congenital toxoplasmosis</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Cytokines - secretion</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>fas Receptor - biosynthesis</subject><subject>fas Receptor - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Mitogen-Activated Protein Kinase 1 - biosynthesis</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 3 - biosynthesis</subject><subject>Mitogen-Activated Protein Kinase 3 - metabolism</subject><subject>Obstetrics and Gynecology</subject><subject>Phosphorylation</subject><subject>Placenta - immunology</subject><subject>Placenta - metabolism</subject><subject>Placenta - parasitology</subject><subject>Placenta - secretion</subject><subject>Placentation</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Parasitic - immunology</subject><subject>Pregnancy Complications, Parasitic - metabolism</subject><subject>Pregnancy Complications, Parasitic - parasitology</subject><subject>Pregnancy Complications, Parasitic - pathology</subject><subject>Protein Processing, Post-Translational</subject><subject>Recombinant Proteins - metabolism</subject><subject>Species Specificity</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma - pathogenicity</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis - immunology</subject><subject>Toxoplasmosis - metabolism</subject><subject>Toxoplasmosis - parasitology</subject><subject>Toxoplasmosis - pathology</subject><subject>Trophoblastic cells</subject><subject>Trophoblasts - immunology</subject><subject>Trophoblasts - metabolism</subject><subject>Trophoblasts - parasitology</subject><subject>Trophoblasts - secretion</subject><subject>Up-Regulation</subject><subject>Virulence</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksFu1DAQhiMEokvhFaq5ILUSu7VjJ6kvCChbiihCKkUcLceZdL3NxsH2lu578wBM2C0gLhyiRM43_4z_f7LsgLMZZ7w8Xs6Gzljsk5nljIsZUzPGigfZhBcinwrO8ofZhHEpppIxuZc9iXHJGFOS54-zvVyySirGJtmPt65tMZCQMx2YwQ_JRxfB9fAGv3qw2HURTJsw0FmLNjnfw3eXFrBw14tuA4eX50fgA6x8g8EkHI8-zqU6glsX1h0pQ0zBuD6Cb-HK33maPK4MXPu-cQ6oWcCOChtIHtICwW6Sv3E9whB86zqEiDYgAS9-_W7QUPeAFmnWAGcmAt4NAWMcRzN9A8PCR3rCZis7v_zAj_O_oKfZo9Z0EZ_t3vvZl7P51en59OLTu_enry-mtmA8TW3FBK9K3pzIVpCLtqxrU9ZNIU2lclUb1VojjCiavLJ5rYSgj1IUUpRVIVUp9rPDrS5d5NsaY9IrF0dHTY9-HTWXUihVlHlBaLlFbfAxBmz1ENzKhI3mTI-J66W-T1yPiWumNCVOhQe7Hut6hc3vsvuICXi-A0y0pmuD6a2Lf7hK8ZOCK-JebTkkR24dBh2tw95i48jrpBvv_j_Ly38kbOd6R11vcINx6dehJ7811zHXTH8e93NcT07uVoI29ydvwuXY</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Angeloni, M.B</creator><creator>Guirelli, P.M</creator><creator>Franco, P.S</creator><creator>Barbosa, B.F</creator><creator>Gomes, A.O</creator><creator>Castro, A.S</creator><creator>Silva, N.M</creator><creator>Martins-Filho, O.A</creator><creator>Mineo, T.W.P</creator><creator>Silva, D.A.O</creator><creator>Mineo, J.R</creator><creator>Ferro, E.A.V</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131101</creationdate><title>Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression</title><author>Angeloni, M.B ; Guirelli, P.M ; Franco, P.S ; Barbosa, B.F ; Gomes, A.O ; Castro, A.S ; Silva, N.M ; Martins-Filho, O.A ; Mineo, T.W.P ; Silva, D.A.O ; Mineo, J.R ; Ferro, E.A.V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-c7031761d84f3004c6bba6bd54a7929ba9fca3a35d27c2b933d27635436754963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Apoptosis</topic><topic>BeWo cells</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Congenital toxoplasmosis</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Cytokines - secretion</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>fas Receptor - biosynthesis</topic><topic>fas Receptor - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Mitogen-Activated Protein Kinase 1 - biosynthesis</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 3 - biosynthesis</topic><topic>Mitogen-Activated Protein Kinase 3 - metabolism</topic><topic>Obstetrics and Gynecology</topic><topic>Phosphorylation</topic><topic>Placenta - immunology</topic><topic>Placenta - metabolism</topic><topic>Placenta - parasitology</topic><topic>Placenta - secretion</topic><topic>Placentation</topic><topic>Pregnancy</topic><topic>Pregnancy Complications, Parasitic - immunology</topic><topic>Pregnancy Complications, Parasitic - metabolism</topic><topic>Pregnancy Complications, Parasitic - parasitology</topic><topic>Pregnancy Complications, Parasitic - pathology</topic><topic>Protein Processing, Post-Translational</topic><topic>Recombinant Proteins - metabolism</topic><topic>Species Specificity</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma - pathogenicity</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis - immunology</topic><topic>Toxoplasmosis - metabolism</topic><topic>Toxoplasmosis - parasitology</topic><topic>Toxoplasmosis - pathology</topic><topic>Trophoblastic cells</topic><topic>Trophoblasts - immunology</topic><topic>Trophoblasts - metabolism</topic><topic>Trophoblasts - parasitology</topic><topic>Trophoblasts - secretion</topic><topic>Up-Regulation</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angeloni, M.B</creatorcontrib><creatorcontrib>Guirelli, P.M</creatorcontrib><creatorcontrib>Franco, P.S</creatorcontrib><creatorcontrib>Barbosa, B.F</creatorcontrib><creatorcontrib>Gomes, A.O</creatorcontrib><creatorcontrib>Castro, A.S</creatorcontrib><creatorcontrib>Silva, N.M</creatorcontrib><creatorcontrib>Martins-Filho, O.A</creatorcontrib><creatorcontrib>Mineo, T.W.P</creatorcontrib><creatorcontrib>Silva, D.A.O</creatorcontrib><creatorcontrib>Mineo, J.R</creatorcontrib><creatorcontrib>Ferro, E.A.V</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angeloni, M.B</au><au>Guirelli, P.M</au><au>Franco, P.S</au><au>Barbosa, B.F</au><au>Gomes, A.O</au><au>Castro, A.S</au><au>Silva, N.M</au><au>Martins-Filho, O.A</au><au>Mineo, T.W.P</au><au>Silva, D.A.O</au><au>Mineo, J.R</au><au>Ferro, E.A.V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>34</volume><issue>11</issue><spage>973</spage><epage>982</epage><pages>973-982</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract>Abstract Introduction Alterations of apoptosis are commonly associated with pregnancy complications and abortion. Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors that are involved in the differential apoptosis of BeWo cells infected with distinct T. gondii strain types. Methods Human trophoblastic cells (BeWo cell line) were infected with RH or ME49 strains, the cytokine production was measured and the phosphorylation of anti-apoptotic ERK1/2 protein was analyzed. Also, cells were treated with different cytokines, infected with RH or ME49 strain, and analyzed for apoptosis index and Fas/CD95 death receptor expression. Results ME49-infected BeWo cells exhibited a predominantly pro-inflammatory cytokine profile, whereas cells infected with RH strain had a higher production of anti-inflammatory cytokines. Also, the incidence of apoptosis was higher in ME49-infected cells, which have been treated with pro-inflammatory cytokines compared to cells infected with RH and treated with anti-inflammatory cytokines. Moreover, Fas/CD95 expression was higher in cells infected with either ME49 or RH strain and treated with pro-inflammatory cytokines compared to anti-inflammatory cytokine treatment. The phosphorylation of ERK1/2 protein increased after 24 h of infection only with the RH strain. Conclusion These results suggest that opposing mechanisms of interference in apoptosis of BeWo cells after infection with RH or ME49 strains of T. gondii can be associated with the differential cytokine profile secreted, the Fas/CD95 expression and the phosphorylated ERK1/2 expression.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24074900</pmid><doi>10.1016/j.placenta.2013.09.005</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis BeWo cells Biological and medical sciences Cell Line Congenital toxoplasmosis Cytokines - genetics Cytokines - metabolism Cytokines - secretion Embryology: invertebrates and vertebrates. Teratology fas Receptor - biosynthesis fas Receptor - metabolism Female Fundamental and applied biological sciences. Psychology Humans Internal Medicine Mitogen-Activated Protein Kinase 1 - biosynthesis Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - biosynthesis Mitogen-Activated Protein Kinase 3 - metabolism Obstetrics and Gynecology Phosphorylation Placenta - immunology Placenta - metabolism Placenta - parasitology Placenta - secretion Placentation Pregnancy Pregnancy Complications, Parasitic - immunology Pregnancy Complications, Parasitic - metabolism Pregnancy Complications, Parasitic - parasitology Pregnancy Complications, Parasitic - pathology Protein Processing, Post-Translational Recombinant Proteins - metabolism Species Specificity Toxoplasma - immunology Toxoplasma - pathogenicity Toxoplasma gondii Toxoplasmosis - immunology Toxoplasmosis - metabolism Toxoplasmosis - parasitology Toxoplasmosis - pathology Trophoblastic cells Trophoblasts - immunology Trophoblasts - metabolism Trophoblasts - parasitology Trophoblasts - secretion Up-Regulation Virulence |
| title | Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression |
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