Growth and regeneration of Alfalfa callus lines after freezing in liquid nitrogen
Alfalfa ( Medicago sativa L.) callus culture lines established from the cotyledons of aseptically germinated seeds were treated with a cryoprotective mixture containing 10% (w/v) polyethylene glycol, 8% (w/v) glucose and 10% (w/v) dimethylsulfoxide (DMSO), frozen at 1°/min to −30°C, and transferred...
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| Veröffentlicht in: | Plant science (Limerick) 1985-12, Vol.42 (2), p.133-140 |
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| container_title | Plant science (Limerick) |
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| creator | Finkle, Bernard J. Ulrich, Jane M. Rains, D.William Stavarek, Susan J. |
| description | Alfalfa (
Medicago sativa L.) callus culture lines established from the cotyledons of aseptically germinated seeds were treated with a cryoprotective mixture containing 10% (w/v) polyethylene glycol, 8% (w/v) glucose and 10% (w/v) dimethylsulfoxide (DMSO), frozen at 1°/min to −30°C, and transferred to liquid nitrogen (LN). Samples in glass tubes were thawed rapidly, rinsed with a simplified Murashige-Skoog medium and placed on a modified Blaydes agar medium for growth comparisons. Variations of experimental treatments, including thawing-bath temperature, use of glass or polypropylene freezing tubes, and exposure time of callus in cryoprotectant solution, were tested. Following treatments, W74RS callus was stored 1 year in LN and, when thawed, grew at approximately the same rate as unfrozen callus. Two other lines survived LN but the cultures grew more slowly, probably indicating some killing of frozen cells. One of these, Regen S, differentiated and grew into normal-appearing, seed-producing plantlets after thawing; the other, a salt-tolerant line, retained its salt-tolerant character. |
| doi_str_mv | 10.1016/0168-9452(85)90153-0 |
| format | Article |
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Medicago sativa L.) callus culture lines established from the cotyledons of aseptically germinated seeds were treated with a cryoprotective mixture containing 10% (w/v) polyethylene glycol, 8% (w/v) glucose and 10% (w/v) dimethylsulfoxide (DMSO), frozen at 1°/min to −30°C, and transferred to liquid nitrogen (LN). Samples in glass tubes were thawed rapidly, rinsed with a simplified Murashige-Skoog medium and placed on a modified Blaydes agar medium for growth comparisons. Variations of experimental treatments, including thawing-bath temperature, use of glass or polypropylene freezing tubes, and exposure time of callus in cryoprotectant solution, were tested. Following treatments, W74RS callus was stored 1 year in LN and, when thawed, grew at approximately the same rate as unfrozen callus. Two other lines survived LN but the cultures grew more slowly, probably indicating some killing of frozen cells. One of these, Regen S, differentiated and grew into normal-appearing, seed-producing plantlets after thawing; the other, a salt-tolerant line, retained its salt-tolerant character.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/0168-9452(85)90153-0</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; cryogenic (liquid nitrogen) preservation ; cryogenics ; cryoprotectant toxicity ; cryoprotective mixtures (polyethylene glycol, glucose, dimethylsulfoxide) ; Economic plant physiology ; Eukaryotic cell cultures ; freezing-tube effects on survival (glass vs. polypropylene plastic) ; Fundamental and applied biological sciences. Psychology ; In vitro culture ; In vitro propagation: entire plant regeneration from tissues and cell cultures ; Medicago sativa ; Medicago sativa (Alfalfa, Lucerne) tissue culture ; Methods. Procedures. Technologies ; Plant cells and fungal cells ; Plant physiology and development ; plant regeneration after freezing ; thawing rate effect on survival ; Tissue cultures, protoplasts</subject><ispartof>Plant science (Limerick), 1985-12, Vol.42 (2), p.133-140</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-62b871b70ed8f2b01491b6861a177027ac80857b9befdad40be1ca9f4dadc9f33</citedby><cites>FETCH-LOGICAL-c366t-62b871b70ed8f2b01491b6861a177027ac80857b9befdad40be1ca9f4dadc9f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-9452(85)90153-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8658784$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Finkle, Bernard J.</creatorcontrib><creatorcontrib>Ulrich, Jane M.</creatorcontrib><creatorcontrib>Rains, D.William</creatorcontrib><creatorcontrib>Stavarek, Susan J.</creatorcontrib><title>Growth and regeneration of Alfalfa callus lines after freezing in liquid nitrogen</title><title>Plant science (Limerick)</title><description>Alfalfa (
Medicago sativa L.) callus culture lines established from the cotyledons of aseptically germinated seeds were treated with a cryoprotective mixture containing 10% (w/v) polyethylene glycol, 8% (w/v) glucose and 10% (w/v) dimethylsulfoxide (DMSO), frozen at 1°/min to −30°C, and transferred to liquid nitrogen (LN). Samples in glass tubes were thawed rapidly, rinsed with a simplified Murashige-Skoog medium and placed on a modified Blaydes agar medium for growth comparisons. Variations of experimental treatments, including thawing-bath temperature, use of glass or polypropylene freezing tubes, and exposure time of callus in cryoprotectant solution, were tested. Following treatments, W74RS callus was stored 1 year in LN and, when thawed, grew at approximately the same rate as unfrozen callus. Two other lines survived LN but the cultures grew more slowly, probably indicating some killing of frozen cells. One of these, Regen S, differentiated and grew into normal-appearing, seed-producing plantlets after thawing; the other, a salt-tolerant line, retained its salt-tolerant character.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cryogenic (liquid nitrogen) preservation</subject><subject>cryogenics</subject><subject>cryoprotectant toxicity</subject><subject>cryoprotective mixtures (polyethylene glycol, glucose, dimethylsulfoxide)</subject><subject>Economic plant physiology</subject><subject>Eukaryotic cell cultures</subject><subject>freezing-tube effects on survival (glass vs. polypropylene plastic)</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In vitro culture</subject><subject>In vitro propagation: entire plant regeneration from tissues and cell cultures</subject><subject>Medicago sativa</subject><subject>Medicago sativa (Alfalfa, Lucerne) tissue culture</subject><subject>Methods. Procedures. Technologies</subject><subject>Plant cells and fungal cells</subject><subject>Plant physiology and development</subject><subject>plant regeneration after freezing</subject><subject>thawing rate effect on survival</subject><subject>Tissue cultures, protoplasts</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNp9kEFLwzAYhoMoOKf_wEMOInqoJm3SpBdhDJ3CQAQ9hzT9MiNduiWtor_ezI0dhYSQ8LzvRx6Ezim5oYSWt2nLrGI8v5L8uiKUFxk5QCMqRZHlOa8O0WiPHKOTGD8IITnnYoReZqH76t-x9g0OsAAPQfeu87izeNJanRY2um2HiFvnIWJtewjYBoAf5xfY-fS-HlyDvetDlwpO0VGKRTjbnWP09nD_On3M5s-zp-lknpmiLPuszGspaC0INNLmNaGsonUpS6qpECQX2kgiuairGmyjG0ZqoEZXlqWLqWxRjNHltncVuvUAsVdLFw20rfbQDVFRxoqSMppAtgVN6GIMYNUquKUO34oStfGnNnLURo6SXP35UyTFLnb9OiYDNmhvXNxnZcmlkCxhd1sM0l8_HQQVjQNvoHEBTK-azv0_5xdKnIR6</recordid><startdate>19851219</startdate><enddate>19851219</enddate><creator>Finkle, Bernard J.</creator><creator>Ulrich, Jane M.</creator><creator>Rains, D.William</creator><creator>Stavarek, Susan J.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19851219</creationdate><title>Growth and regeneration of Alfalfa callus lines after freezing in liquid nitrogen</title><author>Finkle, Bernard J. ; Ulrich, Jane M. ; Rains, D.William ; Stavarek, Susan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-62b871b70ed8f2b01491b6861a177027ac80857b9befdad40be1ca9f4dadc9f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cryogenic (liquid nitrogen) preservation</topic><topic>cryogenics</topic><topic>cryoprotectant toxicity</topic><topic>cryoprotective mixtures (polyethylene glycol, glucose, dimethylsulfoxide)</topic><topic>Economic plant physiology</topic><topic>Eukaryotic cell cultures</topic><topic>freezing-tube effects on survival (glass vs. polypropylene plastic)</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In vitro culture</topic><topic>In vitro propagation: entire plant regeneration from tissues and cell cultures</topic><topic>Medicago sativa</topic><topic>Medicago sativa (Alfalfa, Lucerne) tissue culture</topic><topic>Methods. Procedures. Technologies</topic><topic>Plant cells and fungal cells</topic><topic>Plant physiology and development</topic><topic>plant regeneration after freezing</topic><topic>thawing rate effect on survival</topic><topic>Tissue cultures, protoplasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Finkle, Bernard J.</creatorcontrib><creatorcontrib>Ulrich, Jane M.</creatorcontrib><creatorcontrib>Rains, D.William</creatorcontrib><creatorcontrib>Stavarek, Susan J.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Finkle, Bernard J.</au><au>Ulrich, Jane M.</au><au>Rains, D.William</au><au>Stavarek, Susan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth and regeneration of Alfalfa callus lines after freezing in liquid nitrogen</atitle><jtitle>Plant science (Limerick)</jtitle><date>1985-12-19</date><risdate>1985</risdate><volume>42</volume><issue>2</issue><spage>133</spage><epage>140</epage><pages>133-140</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>Alfalfa (
Medicago sativa L.) callus culture lines established from the cotyledons of aseptically germinated seeds were treated with a cryoprotective mixture containing 10% (w/v) polyethylene glycol, 8% (w/v) glucose and 10% (w/v) dimethylsulfoxide (DMSO), frozen at 1°/min to −30°C, and transferred to liquid nitrogen (LN). Samples in glass tubes were thawed rapidly, rinsed with a simplified Murashige-Skoog medium and placed on a modified Blaydes agar medium for growth comparisons. Variations of experimental treatments, including thawing-bath temperature, use of glass or polypropylene freezing tubes, and exposure time of callus in cryoprotectant solution, were tested. Following treatments, W74RS callus was stored 1 year in LN and, when thawed, grew at approximately the same rate as unfrozen callus. Two other lines survived LN but the cultures grew more slowly, probably indicating some killing of frozen cells. One of these, Regen S, differentiated and grew into normal-appearing, seed-producing plantlets after thawing; the other, a salt-tolerant line, retained its salt-tolerant character.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/0168-9452(85)90153-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant science (Limerick), 1985-12, Vol.42 (2), p.133-140 |
| issn | 0168-9452 1873-2259 |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology cryogenic (liquid nitrogen) preservation cryogenics cryoprotectant toxicity cryoprotective mixtures (polyethylene glycol, glucose, dimethylsulfoxide) Economic plant physiology Eukaryotic cell cultures freezing-tube effects on survival (glass vs. polypropylene plastic) Fundamental and applied biological sciences. Psychology In vitro culture In vitro propagation: entire plant regeneration from tissues and cell cultures Medicago sativa Medicago sativa (Alfalfa, Lucerne) tissue culture Methods. Procedures. Technologies Plant cells and fungal cells Plant physiology and development plant regeneration after freezing thawing rate effect on survival Tissue cultures, protoplasts |
| title | Growth and regeneration of Alfalfa callus lines after freezing in liquid nitrogen |
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