A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3 nanorods, methyl bonding domain protein and anti-his tag antibody
In this work, we fabricated a novel photoelectrochemical immunosensor for assay of DNA methylation, where Bi2S3 nanorods were used as photoelectric conversion material, MBD1 protein (a kind of methyl bonding domain protein) was used as DNA methylation recognizing unit, anti-his tag antibody was used...
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| Veröffentlicht in: | Biosensors & bioelectronics 2014-01, Vol.51, p.103-108 |
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| creator | Yin, Huanshun Sun, Bing Zhou, Yunlei Wang, Mo Xu, Zhenning Fu, Zhengliang Ai, Shiyun |
| description | In this work, we fabricated a novel photoelectrochemical immunosensor for assay of DNA methylation, where Bi2S3 nanorods were used as photoelectric conversion material, MBD1 protein (a kind of methyl bonding domain protein) was used as DNA methylation recognizing unit, anti-his tag antibody was used to further inhibit the photocurrent and increase the detection sensitivity. The results demonstrated that Bi2S3 possessed excellent photoelectron property. The detection conditions, such as Bi2S3 concentration, MBD1 protein concentration, incubation time of MBD1 protein, antibody concentration and antibody incubation time, were optimized. Under optimal experimental conditions, the photocurrent variation was proportional to the logarithm of methylated target DNA concentration from 10−9 to 10–13M with detection limit of 3.5×10–14M (S/N=3). Moreover, the immunosensor presented high detection specificity, even distinguishing single-base mismatched sequence.
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•DNA methylation was detected by a novel photoelectrochemical (PEC) immunosensor.•The photoelectrochemical signal was produced by Bi2S3 nanoparticles.•Methylated DNA was recognized by methyl bonding domain (MBD) protein.•The photoelectrochemical signal decreased after capturing MBD protein and his-tag antibody.•The immunosensor showed a low detection limit of 3.5×10–14M. |
| doi_str_mv | 10.1016/j.bios.2013.07.040 |
| format | Article |
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[Display omitted]
•DNA methylation was detected by a novel photoelectrochemical (PEC) immunosensor.•The photoelectrochemical signal was produced by Bi2S3 nanoparticles.•Methylated DNA was recognized by methyl bonding domain (MBD) protein.•The photoelectrochemical signal decreased after capturing MBD protein and his-tag antibody.•The immunosensor showed a low detection limit of 3.5×10–14M.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2013.07.040</identifier><identifier>PMID: 23948240</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Anti-his tag antibody ; Base Sequence ; Bi2S3 nanomaterial ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Bismuth - chemistry ; DNA - analysis ; DNA - metabolism ; DNA Methylation ; DNA-Binding Proteins - metabolism ; Electrochemical Techniques - methods ; Fundamental and applied biological sciences. Psychology ; Immunoassay - methods ; Limit of Detection ; Methods. Procedures. Technologies ; Methyl bonding domain protein ; Models, Molecular ; Nanotubes - chemistry ; Nanotubes - ultrastructure ; Photoelectrochemical immunosensor ; Recombinant Proteins - metabolism ; Reproducibility of Results ; Sulfides - chemistry ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2014-01, Vol.51, p.103-108</ispartof><rights>2013 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-8da6ac0bfe86aafaa2329a64d2999423db99b9955f44a1a983ffc40a81f069913</citedby><cites>FETCH-LOGICAL-c452t-8da6ac0bfe86aafaa2329a64d2999423db99b9955f44a1a983ffc40a81f069913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2013.07.040$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27822557$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23948240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Huanshun</creatorcontrib><creatorcontrib>Sun, Bing</creatorcontrib><creatorcontrib>Zhou, Yunlei</creatorcontrib><creatorcontrib>Wang, Mo</creatorcontrib><creatorcontrib>Xu, Zhenning</creatorcontrib><creatorcontrib>Fu, Zhengliang</creatorcontrib><creatorcontrib>Ai, Shiyun</creatorcontrib><title>A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3 nanorods, methyl bonding domain protein and anti-his tag antibody</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>In this work, we fabricated a novel photoelectrochemical immunosensor for assay of DNA methylation, where Bi2S3 nanorods were used as photoelectric conversion material, MBD1 protein (a kind of methyl bonding domain protein) was used as DNA methylation recognizing unit, anti-his tag antibody was used to further inhibit the photocurrent and increase the detection sensitivity. The results demonstrated that Bi2S3 possessed excellent photoelectron property. The detection conditions, such as Bi2S3 concentration, MBD1 protein concentration, incubation time of MBD1 protein, antibody concentration and antibody incubation time, were optimized. Under optimal experimental conditions, the photocurrent variation was proportional to the logarithm of methylated target DNA concentration from 10−9 to 10–13M with detection limit of 3.5×10–14M (S/N=3). Moreover, the immunosensor presented high detection specificity, even distinguishing single-base mismatched sequence.
[Display omitted]
•DNA methylation was detected by a novel photoelectrochemical (PEC) immunosensor.•The photoelectrochemical signal was produced by Bi2S3 nanoparticles.•Methylated DNA was recognized by methyl bonding domain (MBD) protein.•The photoelectrochemical signal decreased after capturing MBD protein and his-tag antibody.•The immunosensor showed a low detection limit of 3.5×10–14M.</description><subject>Anti-his tag antibody</subject><subject>Base Sequence</subject><subject>Bi2S3 nanomaterial</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Bismuth - chemistry</subject><subject>DNA - analysis</subject><subject>DNA - metabolism</subject><subject>DNA Methylation</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Electrochemical Techniques - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoassay - methods</subject><subject>Limit of Detection</subject><subject>Methods. Procedures. Technologies</subject><subject>Methyl bonding domain protein</subject><subject>Models, Molecular</subject><subject>Nanotubes - chemistry</subject><subject>Nanotubes - ultrastructure</subject><subject>Photoelectrochemical immunosensor</subject><subject>Recombinant Proteins - metabolism</subject><subject>Reproducibility of Results</subject><subject>Sulfides - chemistry</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcmOEzEQhi0EYjIDL8AB-YLEYTp468USlzCs0ggOwNmq9pI46rYH2w3K-_CgOCTADclWucpf_SrVj9ATStaU0O7Ffj36mNeMUL4m_ZoIcg-t6NDzRjDe3kcrItuuabuOX6DLnPeEkJ5K8hBdMC7FwARZoZ8bHOwPnEuCYrcH7GLCsy27w1Rzg19_3GBji9XFx4BHyLVWH3e7WKKdajlFvbOz1zBhP89LiNmGXDWW7MMWv_LsM8cBQkzR5OuzMh5jMMdvE2fwVS3FYmuEYOotvtn5jAtsfydjNIdH6IGDKdvH53iFvr598-XmfXP76d2Hm81to0XLSjMY6ECT0dmhA3AAjDMJnTBMSllXYkYp62lbJwRQkAN3TgsCA3Wkk5LyK_T8pFsn-rbYXNTss7bTBMHGJSsqBBeUsr6rKDuhOsWck3XqLvkZ0kFRoo7uqL06uqOO7ijSq-pObXp61l_G2Zq_LX_sqMCzMwC5rtQlCNrnf1w_MNa2feVenjhbt_Hd26Sy9jZoa3yqpigT_f_m-AWQabDv</recordid><startdate>20140115</startdate><enddate>20140115</enddate><creator>Yin, Huanshun</creator><creator>Sun, Bing</creator><creator>Zhou, Yunlei</creator><creator>Wang, Mo</creator><creator>Xu, Zhenning</creator><creator>Fu, Zhengliang</creator><creator>Ai, Shiyun</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140115</creationdate><title>A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3 nanorods, methyl bonding domain protein and anti-his tag antibody</title><author>Yin, Huanshun ; Sun, Bing ; Zhou, Yunlei ; Wang, Mo ; Xu, Zhenning ; Fu, Zhengliang ; Ai, Shiyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-8da6ac0bfe86aafaa2329a64d2999423db99b9955f44a1a983ffc40a81f069913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anti-his tag antibody</topic><topic>Base Sequence</topic><topic>Bi2S3 nanomaterial</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Bismuth - chemistry</topic><topic>DNA - analysis</topic><topic>DNA - metabolism</topic><topic>DNA Methylation</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Electrochemical Techniques - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoassay - methods</topic><topic>Limit of Detection</topic><topic>Methods. Procedures. Technologies</topic><topic>Methyl bonding domain protein</topic><topic>Models, Molecular</topic><topic>Nanotubes - chemistry</topic><topic>Nanotubes - ultrastructure</topic><topic>Photoelectrochemical immunosensor</topic><topic>Recombinant Proteins - metabolism</topic><topic>Reproducibility of Results</topic><topic>Sulfides - chemistry</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Huanshun</creatorcontrib><creatorcontrib>Sun, Bing</creatorcontrib><creatorcontrib>Zhou, Yunlei</creatorcontrib><creatorcontrib>Wang, Mo</creatorcontrib><creatorcontrib>Xu, Zhenning</creatorcontrib><creatorcontrib>Fu, Zhengliang</creatorcontrib><creatorcontrib>Ai, Shiyun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Huanshun</au><au>Sun, Bing</au><au>Zhou, Yunlei</au><au>Wang, Mo</au><au>Xu, Zhenning</au><au>Fu, Zhengliang</au><au>Ai, Shiyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3 nanorods, methyl bonding domain protein and anti-his tag antibody</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2014-01-15</date><risdate>2014</risdate><volume>51</volume><spage>103</spage><epage>108</epage><pages>103-108</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>In this work, we fabricated a novel photoelectrochemical immunosensor for assay of DNA methylation, where Bi2S3 nanorods were used as photoelectric conversion material, MBD1 protein (a kind of methyl bonding domain protein) was used as DNA methylation recognizing unit, anti-his tag antibody was used to further inhibit the photocurrent and increase the detection sensitivity. The results demonstrated that Bi2S3 possessed excellent photoelectron property. The detection conditions, such as Bi2S3 concentration, MBD1 protein concentration, incubation time of MBD1 protein, antibody concentration and antibody incubation time, were optimized. Under optimal experimental conditions, the photocurrent variation was proportional to the logarithm of methylated target DNA concentration from 10−9 to 10–13M with detection limit of 3.5×10–14M (S/N=3). Moreover, the immunosensor presented high detection specificity, even distinguishing single-base mismatched sequence.
[Display omitted]
•DNA methylation was detected by a novel photoelectrochemical (PEC) immunosensor.•The photoelectrochemical signal was produced by Bi2S3 nanoparticles.•Methylated DNA was recognized by methyl bonding domain (MBD) protein.•The photoelectrochemical signal decreased after capturing MBD protein and his-tag antibody.•The immunosensor showed a low detection limit of 3.5×10–14M.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>23948240</pmid><doi>10.1016/j.bios.2013.07.040</doi><tpages>6</tpages></addata></record> |
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| subjects | Anti-his tag antibody Base Sequence Bi2S3 nanomaterial Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Bismuth - chemistry DNA - analysis DNA - metabolism DNA Methylation DNA-Binding Proteins - metabolism Electrochemical Techniques - methods Fundamental and applied biological sciences. Psychology Immunoassay - methods Limit of Detection Methods. Procedures. Technologies Methyl bonding domain protein Models, Molecular Nanotubes - chemistry Nanotubes - ultrastructure Photoelectrochemical immunosensor Recombinant Proteins - metabolism Reproducibility of Results Sulfides - chemistry Various methods and equipments |
| title | A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3 nanorods, methyl bonding domain protein and anti-his tag antibody |
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