Brief report: CD24 and CD44 mark human intestinal epithelial cell populations with characteristics of active and facultative stem cells
Recent seminal studies have rapidly advanced the understanding of intestinal epithelial stem cell (IESC) biology in murine models. However, the lack of techniques suitable for isolation and subsequent downstream analysis of IESCs from human tissue has hindered the application of these findings towar...
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| Veröffentlicht in: | Stem cells (Dayton, Ohio) Ohio), 2013-09, Vol.31 (9), p.2024-2030 |
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| container_title | Stem cells (Dayton, Ohio) |
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| creator | Gracz, Adam D. Fuller, Megan K. Wang, Fengchao Li, Linheng Stelzner, Matthias Dunn, James C.Y. Martin, Martin G. Magness, Scott T. |
| description | Recent seminal studies have rapidly advanced the understanding of intestinal epithelial stem cell (IESC) biology in murine models. However, the lack of techniques suitable for isolation and subsequent downstream analysis of IESCs from human tissue has hindered the application of these findings toward the development of novel diagnostics and therapies with direct clinical relevance. This study demonstrates that the cluster of differentiation genes CD24 and CD44 are differentially expressed across LGR5 positive “active” stem cells as well as HOPX positive “facultative” stem cells. Fluorescence‐activated cell sorting enables differential enrichment of LGR5 (CD24−/CD44+) and HOPX (CD24+/CD44+) cells for gene expression analysis and culture. These findings provide the fundamental methodology and basic cell surface signature necessary for isolating and studying intestinal stem cell populations in human physiology and disease. STEM Cells 2013;31:2024‐2030 |
| doi_str_mv | 10.1002/stem.1391 |
| format | Article |
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STEM Cells 2013;31:2024‐2030</description><subject>Adult</subject><subject>Biomarkers - metabolism</subject><subject>CD24 Antigen - metabolism</subject><subject>Cell culture</subject><subject>Cell surface markers</subject><subject>Epithelial Cells - cytology</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Fluorescence‐activated cell sorting</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Hyaluronan Receptors - metabolism</subject><subject>Intestines - cytology</subject><subject>Medical research</subject><subject>Middle Aged</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Tissue‐specific stem cells</subject><issn>1066-5099</issn><issn>1549-4918</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1OHDEQhS0UFAjJIheILGVDFg0uu7ptZ0cGEiIRZZHZtzweW2PoP-zuIE7AtXHPAAukSFm5qvz5qfweIR-BnQBj_DSNrj0BoWGPHEKJukAN6k2uWVUVJdP6gLxL6ZoxwFKpt-SAi7IUmvFD8vAtBudpdEMfx690cc6Rmm6dC0TamnhDN1NrOhq60aUxdKahbgjjxjUhl9Y1DR36YWrMGPou0bt8Re3GRGNHF0N-YRPtPc1t-Ou2yt7YqRnNtp8X34qk92Tfmya5D0_nEVl-v1guLour3z9-Ls6uCoscoIAKV0qsjLRaIpjSAoKEFaLLQ68911YxyddK-zWTxkqBSvHKA2dccBBH5HgnO8T-dso_qtuQ5gVM5_op1YAokEkN-F-okJLJGf38Cr3up5i9mimhuSqrcqa-7Cgb-5Si8_UQQ7b4vgZWzznWsx31nGNmPz0pTqvWrV_I5-AycLoD7kLj7v-tVP9ZXvzaSj4C2Eumug</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Gracz, Adam D.</creator><creator>Fuller, Megan K.</creator><creator>Wang, Fengchao</creator><creator>Li, Linheng</creator><creator>Stelzner, Matthias</creator><creator>Dunn, James C.Y.</creator><creator>Martin, Martin G.</creator><creator>Magness, Scott T.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201309</creationdate><title>Brief report: CD24 and CD44 mark human intestinal epithelial cell populations with characteristics of active and facultative stem cells</title><author>Gracz, Adam D. ; 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adult Biomarkers - metabolism CD24 Antigen - metabolism Cell culture Cell surface markers Epithelial Cells - cytology Female Flow cytometry Fluorescence‐activated cell sorting Gene expression Humans Hyaluronan Receptors - metabolism Intestines - cytology Medical research Middle Aged Stem cells Stem Cells - cytology Stem Cells - metabolism Tissue‐specific stem cells |
| title | Brief report: CD24 and CD44 mark human intestinal epithelial cell populations with characteristics of active and facultative stem cells |
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