Rapid determination of catecholamines in urine samples by nonaqueous microchip electrophoresis with LIF detection
A method was developed for the rapid separation of catecholamines by nonaqueous microchip electrophoresis (NAMCE) with LIF detection, A homemade pump‐free negative pressure sampling device was used for rapid bias‐free sampling in NAMCE, the injection time was 0.5 s and the electrophoresis separation...
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| Veröffentlicht in: | Journal of separation science 2013-10, Vol.36 (20), p.3419-3425 |
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| creator | Hu, Hongmei Li, Zhenhua Zhang, Xiaoning Xu, Chunxiu Guo, Yuanming |
| description | A method was developed for the rapid separation of catecholamines by nonaqueous microchip electrophoresis (NAMCE) with LIF detection, A homemade pump‐free negative pressure sampling device was used for rapid bias‐free sampling in NAMCE, the injection time was 0.5 s and the electrophoresis separation conditions were optimized. Under the optimized conditions, the samples were separated completely in |
| doi_str_mv | 10.1002/jssc.201300342 |
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Under the optimized conditions, the samples were separated completely in <1 min. The average migration times of the epinephrine (E), dopamine (DA), and norepinephrine (NE) were 34.26, 43.81, and 50.07 s, with an RSD of 1.05, 1.26, and 0.89% (n = 7), respectively. The linearity of the method ranged from 0.0125 to 2.0 mg/L for E and 0.025∼4.0 mg/L for DA and NE, with correlation coefficients ranging between 0.9978 and 0.9986. The detection limits of E, DA, and NE were 2.5, 5.0, and 5.0 μg/L, respectively. The recoveries of E, DA, and NE in spiked urine samples were between 86 and 103%, with RSDs of 4.5∼6.8% (n = 5). The proposed NAMCE with LIF detection combined with a pump‐free negative pressure sampling device is a simple, inexpensive, energy efficient, miniaturized system that can be successfully applied for the determination of catecholamines in urine samples.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.201300342</identifier><identifier>PMID: 24038935</identifier><language>eng</language><publisher>Weinheim: Blackwell Publishing Ltd</publisher><subject>Amino acids ; Analytical chemistry ; Biological and medical sciences ; Catecholamines ; Catecholamines - urine ; Electrophoresis, Microchip - instrumentation ; Electrophoresis, Microchip - methods ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Method development ; Microchip electrophoresis ; Miscellaneous. Technology ; Neurotransmitter Agents - urine ; Nonaqueous ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Proteins ; Urine ; Urine samples</subject><ispartof>Journal of separation science, 2013-10, Vol.36 (20), p.3419-3425</ispartof><rights>2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2014 INIST-CNRS</rights><rights>2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3517-8a40c75da50adaf33df5805a631543d05729be07b4b7d17ee8834a0e94566c373</citedby><cites>FETCH-LOGICAL-c3517-8a40c75da50adaf33df5805a631543d05729be07b4b7d17ee8834a0e94566c373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.201300342$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.201300342$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27863969$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24038935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Hongmei</creatorcontrib><creatorcontrib>Li, Zhenhua</creatorcontrib><creatorcontrib>Zhang, Xiaoning</creatorcontrib><creatorcontrib>Xu, Chunxiu</creatorcontrib><creatorcontrib>Guo, Yuanming</creatorcontrib><title>Rapid determination of catecholamines in urine samples by nonaqueous microchip electrophoresis with LIF detection</title><title>Journal of separation science</title><addtitle>J. Sep. Science</addtitle><description>A method was developed for the rapid separation of catecholamines by nonaqueous microchip electrophoresis (NAMCE) with LIF detection, A homemade pump‐free negative pressure sampling device was used for rapid bias‐free sampling in NAMCE, the injection time was 0.5 s and the electrophoresis separation conditions were optimized. Under the optimized conditions, the samples were separated completely in <1 min. The average migration times of the epinephrine (E), dopamine (DA), and norepinephrine (NE) were 34.26, 43.81, and 50.07 s, with an RSD of 1.05, 1.26, and 0.89% (n = 7), respectively. The linearity of the method ranged from 0.0125 to 2.0 mg/L for E and 0.025∼4.0 mg/L for DA and NE, with correlation coefficients ranging between 0.9978 and 0.9986. The detection limits of E, DA, and NE were 2.5, 5.0, and 5.0 μg/L, respectively. The recoveries of E, DA, and NE in spiked urine samples were between 86 and 103%, with RSDs of 4.5∼6.8% (n = 5). The proposed NAMCE with LIF detection combined with a pump‐free negative pressure sampling device is a simple, inexpensive, energy efficient, miniaturized system that can be successfully applied for the determination of catecholamines in urine samples.</description><subject>Amino acids</subject><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Catecholamines</subject><subject>Catecholamines - urine</subject><subject>Electrophoresis, Microchip - instrumentation</subject><subject>Electrophoresis, Microchip - methods</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Method development</subject><subject>Microchip electrophoresis</subject><subject>Miscellaneous. Technology</subject><subject>Neurotransmitter Agents - urine</subject><subject>Nonaqueous</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Proteins</subject><subject>Urine</subject><subject>Urine samples</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxSMEoh9w5YgsISQuWcaxHTtHtKKlaFWgBXG0Zh1H6yWJUztR2f--TndZJC6cPBr95s3zvCx7RWFBAYr32xjNogDKABgvnmSntKQirxjlT481lCfZWYxbACpVBc-zk4IDUxUTp9ndDQ6uJrUdbehcj6PzPfENMThas_EtpqaNxPVkCqkiEbuhTY31jvS-x7vJ-imSzpngzcYNxLbWjMEPGx9sdJHcu3FDVlcXjxvMrP4ie9ZgG-3Lw3ue_bj4-H35KV99ubxafljlhgkqc4UcjBQ1CsAaG8bqRigQWDIqOKtByKJaW5BrvpY1ldYqxTiCrbgoS8MkO8_e7XWH4JPNOOrORWPbFvvZs6acMw6lqIqEvvkH3fop9MndIyUoBzFTiz2V_hpjsI0egusw7DQFPYeh5zD0MYw08PogO607Wx_xP9dPwNsDgNFg2wTsjYt_OalKVpVV4vieu3et3f1nrf58e7tkXMwXyPdjLo7293EMwy9dSiaF_nl9qb9dK1BfVzdasQervrH0</recordid><startdate>201310</startdate><enddate>201310</enddate><creator>Hu, Hongmei</creator><creator>Li, Zhenhua</creator><creator>Zhang, Xiaoning</creator><creator>Xu, Chunxiu</creator><creator>Guo, Yuanming</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>201310</creationdate><title>Rapid determination of catecholamines in urine samples by nonaqueous microchip electrophoresis with LIF detection</title><author>Hu, Hongmei ; Li, Zhenhua ; Zhang, Xiaoning ; Xu, Chunxiu ; Guo, Yuanming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3517-8a40c75da50adaf33df5805a631543d05729be07b4b7d17ee8834a0e94566c373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino acids</topic><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Catecholamines</topic><topic>Catecholamines - urine</topic><topic>Electrophoresis, Microchip - instrumentation</topic><topic>Electrophoresis, Microchip - methods</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Method development</topic><topic>Microchip electrophoresis</topic><topic>Miscellaneous. Technology</topic><topic>Neurotransmitter Agents - urine</topic><topic>Nonaqueous</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Proteins</topic><topic>Urine</topic><topic>Urine samples</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Hongmei</creatorcontrib><creatorcontrib>Li, Zhenhua</creatorcontrib><creatorcontrib>Zhang, Xiaoning</creatorcontrib><creatorcontrib>Xu, Chunxiu</creatorcontrib><creatorcontrib>Guo, Yuanming</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Hongmei</au><au>Li, Zhenhua</au><au>Zhang, Xiaoning</au><au>Xu, Chunxiu</au><au>Guo, Yuanming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid determination of catecholamines in urine samples by nonaqueous microchip electrophoresis with LIF detection</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2013-10</date><risdate>2013</risdate><volume>36</volume><issue>20</issue><spage>3419</spage><epage>3425</epage><pages>3419-3425</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>A method was developed for the rapid separation of catecholamines by nonaqueous microchip electrophoresis (NAMCE) with LIF detection, A homemade pump‐free negative pressure sampling device was used for rapid bias‐free sampling in NAMCE, the injection time was 0.5 s and the electrophoresis separation conditions were optimized. Under the optimized conditions, the samples were separated completely in <1 min. The average migration times of the epinephrine (E), dopamine (DA), and norepinephrine (NE) were 34.26, 43.81, and 50.07 s, with an RSD of 1.05, 1.26, and 0.89% (n = 7), respectively. The linearity of the method ranged from 0.0125 to 2.0 mg/L for E and 0.025∼4.0 mg/L for DA and NE, with correlation coefficients ranging between 0.9978 and 0.9986. The detection limits of E, DA, and NE were 2.5, 5.0, and 5.0 μg/L, respectively. The recoveries of E, DA, and NE in spiked urine samples were between 86 and 103%, with RSDs of 4.5∼6.8% (n = 5). The proposed NAMCE with LIF detection combined with a pump‐free negative pressure sampling device is a simple, inexpensive, energy efficient, miniaturized system that can be successfully applied for the determination of catecholamines in urine samples.</abstract><cop>Weinheim</cop><pub>Blackwell Publishing Ltd</pub><pmid>24038935</pmid><doi>10.1002/jssc.201300342</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino acids Analytical chemistry Biological and medical sciences Catecholamines Catecholamines - urine Electrophoresis, Microchip - instrumentation Electrophoresis, Microchip - methods Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Method development Microchip electrophoresis Miscellaneous. Technology Neurotransmitter Agents - urine Nonaqueous Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Proteins Urine Urine samples |
| title | Rapid determination of catecholamines in urine samples by nonaqueous microchip electrophoresis with LIF detection |
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