Development, Purification, and Characterization of Monoclonal Antibodies Against Recombinant Histidine-rich Protein 3 of Plasmodium falciparum
In the present study, monoclonal antibodies (MAbs) against recombinant histidine-rich protein (rHRP3) were developed and assessed for their potential in detection of Plasmodium falciparum HRP3. Hybridomas were obtained by fusion of Sp2/0 mouse myeloma cells and spleen cells from the mouse immunized...
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| Veröffentlicht in: | Monoclonal antibodies in immunodiagnosis and immunotherapy 2013-10, Vol.32 (5), p.341-348 |
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| creator | Palani, B. Ranjini, S. Shiva Jayaprakash, N.S. Vijayalakshmi, M.A. |
| description | In the present study, monoclonal antibodies (MAbs) against recombinant histidine-rich protein (rHRP3) were developed and assessed for their potential in detection of
Plasmodium falciparum
HRP3. Hybridomas were obtained by fusion of Sp2/0 mouse myeloma cells and spleen cells from the mouse immunized with purified rHRP3. Three MAbs (IgG1 isotype) specific to rHRP3 were established and characterized by enzyme-linked immunosorbent assay (ELISA) and immunoblotting for sensitivity and specificity. Purification of MAbs from hybridoma cell culture supernatant and PAbs from rabbit anti-serum were carried out using Phenylpropylamine (PPA) HyperCel
™
sorbent. The MAbs were able to detect rHRP3 and the HRP3 from
P. falciparum
spent medium. Sandwich ELISA was developed to quantify HRP3 in the spent medium of
P. falciparum
culture. The generated MAbs could be potentially used in immuno-based diagnostic systems for the detection of
P. falciparum
HRP. |
| doi_str_mv | 10.1089/mab.2013.0018 |
| format | Article |
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Plasmodium falciparum
HRP3. Hybridomas were obtained by fusion of Sp2/0 mouse myeloma cells and spleen cells from the mouse immunized with purified rHRP3. Three MAbs (IgG1 isotype) specific to rHRP3 were established and characterized by enzyme-linked immunosorbent assay (ELISA) and immunoblotting for sensitivity and specificity. Purification of MAbs from hybridoma cell culture supernatant and PAbs from rabbit anti-serum were carried out using Phenylpropylamine (PPA) HyperCel
™
sorbent. The MAbs were able to detect rHRP3 and the HRP3 from
P. falciparum
spent medium. Sandwich ELISA was developed to quantify HRP3 in the spent medium of
P. falciparum
culture. The generated MAbs could be potentially used in immuno-based diagnostic systems for the detection of
P. falciparum
HRP.</description><identifier>ISSN: 2167-9436</identifier><identifier>EISSN: 2167-9436</identifier><identifier>DOI: 10.1089/mab.2013.0018</identifier><identifier>PMID: 24111866</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - genetics ; Antigens, Protozoan - immunology ; Enzyme-Linked Immunosorbent Assay - methods ; Hybridomas - cytology ; Hybridomas - metabolism ; Immunoblotting ; Malaria - diagnosis ; Mice ; Original Articles ; Plasmodium falciparum - immunology ; Plasmodium falciparum - metabolism ; Protozoan Proteins - immunology ; Rabbits ; Recombinant Proteins - immunology ; Sensitivity and Specificity ; Spleen - cytology ; Tumor Cells, Cultured</subject><ispartof>Monoclonal antibodies in immunodiagnosis and immunotherapy, 2013-10, Vol.32 (5), p.341-348</ispartof><rights>2013, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c252t-1ef221c87a36f4fd8b5de8bcc00c93495cceda945a2b26ca9ed63d2a83009b123</citedby><cites>FETCH-LOGICAL-c252t-1ef221c87a36f4fd8b5de8bcc00c93495cceda945a2b26ca9ed63d2a83009b123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24111866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palani, B.</creatorcontrib><creatorcontrib>Ranjini, S. Shiva</creatorcontrib><creatorcontrib>Jayaprakash, N.S.</creatorcontrib><creatorcontrib>Vijayalakshmi, M.A.</creatorcontrib><title>Development, Purification, and Characterization of Monoclonal Antibodies Against Recombinant Histidine-rich Protein 3 of Plasmodium falciparum</title><title>Monoclonal antibodies in immunodiagnosis and immunotherapy</title><addtitle>Monoclon Antib Immunodiagn Immunother</addtitle><description>In the present study, monoclonal antibodies (MAbs) against recombinant histidine-rich protein (rHRP3) were developed and assessed for their potential in detection of
Plasmodium falciparum
HRP3. Hybridomas were obtained by fusion of Sp2/0 mouse myeloma cells and spleen cells from the mouse immunized with purified rHRP3. Three MAbs (IgG1 isotype) specific to rHRP3 were established and characterized by enzyme-linked immunosorbent assay (ELISA) and immunoblotting for sensitivity and specificity. Purification of MAbs from hybridoma cell culture supernatant and PAbs from rabbit anti-serum were carried out using Phenylpropylamine (PPA) HyperCel
™
sorbent. The MAbs were able to detect rHRP3 and the HRP3 from
P. falciparum
spent medium. Sandwich ELISA was developed to quantify HRP3 in the spent medium of
P. falciparum
culture. The generated MAbs could be potentially used in immuno-based diagnostic systems for the detection of
P. falciparum
HRP.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Hybridomas - cytology</subject><subject>Hybridomas - metabolism</subject><subject>Immunoblotting</subject><subject>Malaria - diagnosis</subject><subject>Mice</subject><subject>Original Articles</subject><subject>Plasmodium falciparum - immunology</subject><subject>Plasmodium falciparum - metabolism</subject><subject>Protozoan Proteins - immunology</subject><subject>Rabbits</subject><subject>Recombinant Proteins - immunology</subject><subject>Sensitivity and Specificity</subject><subject>Spleen - cytology</subject><subject>Tumor Cells, Cultured</subject><issn>2167-9436</issn><issn>2167-9436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EolXpkSvykUOz-CPJJsfVAi1SESsE52hsT-ig2F5sBwl-BL-5CVsQN-YwX3rmPczL2HMpNlJ0_SsPZqOE1BshZPeInSvZbqu-1u3jf_ozdpnzV7HEViqpm6fsTNVSyq5tz9mv1_gdp3j0GMoVP8yJRrJQKIYrDsHx_R0ksAUT_fy95XHk72OIdooBJr4LhUx0hJnvvgCFXPhHtNEbChAKv6FcyFHAKpG944cUC1LgelU5TJD9cjp7PsJk6Qhp9s_Yk2XIePlQL9jnt28-7W-q2w_X7_a728qqRpVK4qiUtN0WdDvWo-tM47Az1gphe133jbXooK8bUEa1Fnp0rXYKOi1Eb6TSF-zlSfeY4rcZcxk8ZYvTBAHjnAdZ17oWzZIWtDqhNsWcE47DMZGH9GOQYlhdGBYXhtWFYXVh4V88SM_Go_tL__n5AugTsK4hhInQYCr_kb0Hm3yWrQ</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Palani, B.</creator><creator>Ranjini, S. Shiva</creator><creator>Jayaprakash, N.S.</creator><creator>Vijayalakshmi, M.A.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131001</creationdate><title>Development, Purification, and Characterization of Monoclonal Antibodies Against Recombinant Histidine-rich Protein 3 of Plasmodium falciparum</title><author>Palani, B. ; Ranjini, S. Shiva ; Jayaprakash, N.S. ; Vijayalakshmi, M.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c252t-1ef221c87a36f4fd8b5de8bcc00c93495cceda945a2b26ca9ed63d2a83009b123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Hybridomas - cytology</topic><topic>Hybridomas - metabolism</topic><topic>Immunoblotting</topic><topic>Malaria - diagnosis</topic><topic>Mice</topic><topic>Original Articles</topic><topic>Plasmodium falciparum - immunology</topic><topic>Plasmodium falciparum - metabolism</topic><topic>Protozoan Proteins - immunology</topic><topic>Rabbits</topic><topic>Recombinant Proteins - immunology</topic><topic>Sensitivity and Specificity</topic><topic>Spleen - cytology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palani, B.</creatorcontrib><creatorcontrib>Ranjini, S. Shiva</creatorcontrib><creatorcontrib>Jayaprakash, N.S.</creatorcontrib><creatorcontrib>Vijayalakshmi, M.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Monoclonal antibodies in immunodiagnosis and immunotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palani, B.</au><au>Ranjini, S. Shiva</au><au>Jayaprakash, N.S.</au><au>Vijayalakshmi, M.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development, Purification, and Characterization of Monoclonal Antibodies Against Recombinant Histidine-rich Protein 3 of Plasmodium falciparum</atitle><jtitle>Monoclonal antibodies in immunodiagnosis and immunotherapy</jtitle><addtitle>Monoclon Antib Immunodiagn Immunother</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>32</volume><issue>5</issue><spage>341</spage><epage>348</epage><pages>341-348</pages><issn>2167-9436</issn><eissn>2167-9436</eissn><abstract>In the present study, monoclonal antibodies (MAbs) against recombinant histidine-rich protein (rHRP3) were developed and assessed for their potential in detection of
Plasmodium falciparum
HRP3. Hybridomas were obtained by fusion of Sp2/0 mouse myeloma cells and spleen cells from the mouse immunized with purified rHRP3. Three MAbs (IgG1 isotype) specific to rHRP3 were established and characterized by enzyme-linked immunosorbent assay (ELISA) and immunoblotting for sensitivity and specificity. Purification of MAbs from hybridoma cell culture supernatant and PAbs from rabbit anti-serum were carried out using Phenylpropylamine (PPA) HyperCel
™
sorbent. The MAbs were able to detect rHRP3 and the HRP3 from
P. falciparum
spent medium. Sandwich ELISA was developed to quantify HRP3 in the spent medium of
P. falciparum
culture. The generated MAbs could be potentially used in immuno-based diagnostic systems for the detection of
P. falciparum
HRP.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>24111866</pmid><doi>10.1089/mab.2013.0018</doi><tpages>8</tpages></addata></record> |
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| ispartof | Monoclonal antibodies in immunodiagnosis and immunotherapy, 2013-10, Vol.32 (5), p.341-348 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - genetics Antigens, Protozoan - immunology Enzyme-Linked Immunosorbent Assay - methods Hybridomas - cytology Hybridomas - metabolism Immunoblotting Malaria - diagnosis Mice Original Articles Plasmodium falciparum - immunology Plasmodium falciparum - metabolism Protozoan Proteins - immunology Rabbits Recombinant Proteins - immunology Sensitivity and Specificity Spleen - cytology Tumor Cells, Cultured |
| title | Development, Purification, and Characterization of Monoclonal Antibodies Against Recombinant Histidine-rich Protein 3 of Plasmodium falciparum |
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