Improvement of short tandem repeat analysis of samples highly contaminated by humic acid

Abstract We investigated several methods for obtaining successful short tandem repeat (STR) results from high-humic acid (HA)-content samples. DNA purification efficiency was tested for QIAquick® PCR Purification, QIAamp® DNA Investigator and Prepfiler™ Forensic DNA Extraction kits. HA-removal capac...

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Veröffentlicht in:	Journal of forensic and legal medicine 2013-10, Vol.20 (7), p.922-928
Hauptverfasser:	Seo, Seung Bum, PhD, Jin, Hong Xuan, MD, Lee, Hye Young, BS, Ge, Jianye, PhD, King, Jonathan L., MS, Lyoo, Sung Hee, BS, Shin, Dong Hoon, MD, PhD, Lee, Soong Deok, MD, PhD
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Deoxyribonucleic acid DNA DNA - isolation & purification DNA Contamination DNA Fingerprinting - instrumentation DNA Fingerprinting - methods DNA recovery Forensic DNA typing Forensic medicine Humans Humic acid Humic Substances Inhibitor Microsatellite Repeats Molecular biology Pathology Polymerase Chain Reaction Short tandem repeat Soils
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