Serum-free culture of rat proximal tubule cells with enhanced function on chitosan

The proximal tubule performs a variety of important renal functions and is the major site for nutrient reabsorption. The purpose of this study is to culture rat renal proximal tubule cells (PTCs) on chitosan without serum to maintain a transcellular pathway to transport water and ions effectively wi...

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Veröffentlicht in:	Acta biomaterialia 2013-11, Vol.9 (11), p.8942-8951
Hauptverfasser:	Chang, Shao-Hsuan, Chiang, I-Ni, Chen, Yi-Hsin, Young, Tai-Horng
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - metabolism Animals Cadherins - metabolism Cell Adhesion - drug effects Cell Culture Techniques - methods Cell Proliferation - drug effects Cell Shape - drug effects Cells, Cultured Chitosan Chitosan - pharmacology Collagen Collagen - pharmacology Culture Media, Serum-Free - pharmacology Dome formation Electric Impedance electrical resistance Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism fetal bovine serum heparin heparin lyase Heparin Lyase - pharmacology ion transport ions Kidney Tubules, Proximal - cytology Kidney Tubules, Proximal - drug effects Kidney Tubules, Proximal - enzymology Male mitogen-activated protein kinase proteoglycans Proximal tubule cells (PTCs) Rats Rats, Wistar Serum Sodium-Potassium-Exchanging ATPase - metabolism tight junctions Tight Junctions - drug effects Tight Junctions - metabolism tissue engineering viability Zonula Occludens-1 Protein - metabolism
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