Anti-tumor activity of the regenerated triple-helical polysaccharide from Dictyophora indusiata
A triple helical polysaccharide (PD3) was isolated from Dictyophora indusiata. After denaturation in dilute NaOH solution (0.3M) and renaturation by sequential dialysis, regenerated polysaccharide (RPD3) was obtained. The physico-chemical properties of RPD3 including intrinsic viscosity [η], molecul...
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| Veröffentlicht in: | International journal of biological macromolecules 2013-10, Vol.61, p.453-458 |
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| creator | Deng, Chao Fu, Haitian Teng, Liping Hu, Zhun Xu, Xiaofei Chen, Jinghua Ren, Tianli |
| description | A triple helical polysaccharide (PD3) was isolated from Dictyophora indusiata. After denaturation in dilute NaOH solution (0.3M) and renaturation by sequential dialysis, regenerated polysaccharide (RPD3) was obtained. The physico-chemical properties of RPD3 including intrinsic viscosity [η], molecular weight (Mw) and optical rotation were similar to those of PD3, which suggested that RPD3 also had a triple helical structure after denaturation–renaturation. However, different intrinsic viscosity dependence on the concentration of NaOH solution was noted in PD3 and RPD3, which indicated that RPD3 had lower chain tightness compared with PD3. The anti-tumor activity of this polysaccharide after denaturation–renaturation treatment was further investigated. Both PD3 and RPD3 showed no direct cytotoxicity against S-180 cells in vitro but behaved anti-tumor activity in vivo. Meanwhile, RPD3 in high-dose group showed much higher anti-tumor activity than that of PD3, suggesting that the denaturation–renaturation treatment improved the bioactivity of the polysaccharide from D. indusiata. |
| doi_str_mv | 10.1016/j.ijbiomac.2013.08.007 |
| format | Article |
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After denaturation in dilute NaOH solution (0.3M) and renaturation by sequential dialysis, regenerated polysaccharide (RPD3) was obtained. The physico-chemical properties of RPD3 including intrinsic viscosity [η], molecular weight (Mw) and optical rotation were similar to those of PD3, which suggested that RPD3 also had a triple helical structure after denaturation–renaturation. However, different intrinsic viscosity dependence on the concentration of NaOH solution was noted in PD3 and RPD3, which indicated that RPD3 had lower chain tightness compared with PD3. The anti-tumor activity of this polysaccharide after denaturation–renaturation treatment was further investigated. Both PD3 and RPD3 showed no direct cytotoxicity against S-180 cells in vitro but behaved anti-tumor activity in vivo. Meanwhile, RPD3 in high-dose group showed much higher anti-tumor activity than that of PD3, suggesting that the denaturation–renaturation treatment improved the bioactivity of the polysaccharide from D. indusiata.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2013.08.007</identifier><identifier>PMID: 23973497</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Anti-tumor activity ; antineoplastic agents ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - pharmacology ; Antineoplastic Agents - toxicity ; Basidiomycota - chemistry ; Body Weight - drug effects ; Cell Line, Tumor ; Cytokines - blood ; Cytokines - metabolism ; cytotoxicity ; denaturation ; Denaturation–renaturation ; dialysis ; Dictyophora ; Dictyophora indusiata polysaccharide ; Fungal Polysaccharides - chemistry ; Fungal Polysaccharides - pharmacology ; Fungal Polysaccharides - toxicity ; Male ; Mice ; Molecular Weight ; Neoplasms - immunology ; Neoplasms - metabolism ; Neoplasms - pathology ; polysaccharides ; sodium hydroxide ; Viscosity</subject><ispartof>International journal of biological macromolecules, 2013-10, Vol.61, p.453-458</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-3e7e7171151b9232f9ec375aebdf47087b2c1ed3e951af530f3f49b476231dec3</citedby><cites>FETCH-LOGICAL-c392t-3e7e7171151b9232f9ec375aebdf47087b2c1ed3e951af530f3f49b476231dec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2013.08.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23973497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Chao</creatorcontrib><creatorcontrib>Fu, Haitian</creatorcontrib><creatorcontrib>Teng, Liping</creatorcontrib><creatorcontrib>Hu, Zhun</creatorcontrib><creatorcontrib>Xu, Xiaofei</creatorcontrib><creatorcontrib>Chen, Jinghua</creatorcontrib><creatorcontrib>Ren, Tianli</creatorcontrib><title>Anti-tumor activity of the regenerated triple-helical polysaccharide from Dictyophora indusiata</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>A triple helical polysaccharide (PD3) was isolated from Dictyophora indusiata. After denaturation in dilute NaOH solution (0.3M) and renaturation by sequential dialysis, regenerated polysaccharide (RPD3) was obtained. The physico-chemical properties of RPD3 including intrinsic viscosity [η], molecular weight (Mw) and optical rotation were similar to those of PD3, which suggested that RPD3 also had a triple helical structure after denaturation–renaturation. However, different intrinsic viscosity dependence on the concentration of NaOH solution was noted in PD3 and RPD3, which indicated that RPD3 had lower chain tightness compared with PD3. The anti-tumor activity of this polysaccharide after denaturation–renaturation treatment was further investigated. Both PD3 and RPD3 showed no direct cytotoxicity against S-180 cells in vitro but behaved anti-tumor activity in vivo. Meanwhile, RPD3 in high-dose group showed much higher anti-tumor activity than that of PD3, suggesting that the denaturation–renaturation treatment improved the bioactivity of the polysaccharide from D. indusiata.</description><subject>Animals</subject><subject>Anti-tumor activity</subject><subject>antineoplastic agents</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Antineoplastic Agents - toxicity</subject><subject>Basidiomycota - chemistry</subject><subject>Body Weight - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cytokines - blood</subject><subject>Cytokines - metabolism</subject><subject>cytotoxicity</subject><subject>denaturation</subject><subject>Denaturation–renaturation</subject><subject>dialysis</subject><subject>Dictyophora</subject><subject>Dictyophora indusiata polysaccharide</subject><subject>Fungal Polysaccharides - chemistry</subject><subject>Fungal Polysaccharides - pharmacology</subject><subject>Fungal Polysaccharides - toxicity</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Neoplasms - immunology</subject><subject>Neoplasms - metabolism</subject><subject>Neoplasms - pathology</subject><subject>polysaccharides</subject><subject>sodium hydroxide</subject><subject>Viscosity</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EotvCKxQfuSTYcRLHN6oCBakSB-jZcuxxd1ZJHGyn0r49Xm3LldNIo-__Z_QRcs1ZzRnvPx1qPIwYZmPrhnFRs6FmTL4iOz5IVTHGxGuyY7zl1cAFuyCXKR3Ktu_48JZcNEJJ0Sq5I_pmyVjlbQ6RGpvxCfORBk_zHmiER1ggmgyO5ojrBNUeJrRmomuYjslYuzcRHVAfw0y_oM3HsO5DNBQXtyU02bwjb7yZErx_nlfk4dvX37ffq_ufdz9ub-4rK1STKwESJJecd3xUjWi8AitkZ2B0vpVskGNjOTgBquPGd4J54Vs1trJvBHeFvSIfz71rDH82SFnPmCxMk1kgbEnzthViaPqOFbQ_ozaGlCJ4vUacTTxqzvRJrj7oF7n6JFezQRe5JXj9fGMbZ3D_Yi82C_DhDHgTtHmMmPTDr9LQF_OdauWJ-HwmoLh4Qog6WYTFgsMINmsX8H9f_AWyXZkP</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Deng, Chao</creator><creator>Fu, Haitian</creator><creator>Teng, Liping</creator><creator>Hu, Zhun</creator><creator>Xu, Xiaofei</creator><creator>Chen, Jinghua</creator><creator>Ren, Tianli</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131001</creationdate><title>Anti-tumor activity of the regenerated triple-helical polysaccharide from Dictyophora indusiata</title><author>Deng, Chao ; Fu, Haitian ; Teng, Liping ; Hu, Zhun ; Xu, Xiaofei ; Chen, Jinghua ; Ren, Tianli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-3e7e7171151b9232f9ec375aebdf47087b2c1ed3e951af530f3f49b476231dec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Anti-tumor activity</topic><topic>antineoplastic agents</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Antineoplastic Agents - toxicity</topic><topic>Basidiomycota - chemistry</topic><topic>Body Weight - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cytokines - blood</topic><topic>Cytokines - metabolism</topic><topic>cytotoxicity</topic><topic>denaturation</topic><topic>Denaturation–renaturation</topic><topic>dialysis</topic><topic>Dictyophora</topic><topic>Dictyophora indusiata polysaccharide</topic><topic>Fungal Polysaccharides - chemistry</topic><topic>Fungal Polysaccharides - pharmacology</topic><topic>Fungal Polysaccharides - toxicity</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular Weight</topic><topic>Neoplasms - immunology</topic><topic>Neoplasms - metabolism</topic><topic>Neoplasms - pathology</topic><topic>polysaccharides</topic><topic>sodium hydroxide</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Chao</creatorcontrib><creatorcontrib>Fu, Haitian</creatorcontrib><creatorcontrib>Teng, Liping</creatorcontrib><creatorcontrib>Hu, Zhun</creatorcontrib><creatorcontrib>Xu, Xiaofei</creatorcontrib><creatorcontrib>Chen, Jinghua</creatorcontrib><creatorcontrib>Ren, Tianli</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Chao</au><au>Fu, Haitian</au><au>Teng, Liping</au><au>Hu, Zhun</au><au>Xu, Xiaofei</au><au>Chen, Jinghua</au><au>Ren, Tianli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-tumor activity of the regenerated triple-helical polysaccharide from Dictyophora indusiata</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>61</volume><spage>453</spage><epage>458</epage><pages>453-458</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>A triple helical polysaccharide (PD3) was isolated from Dictyophora indusiata. After denaturation in dilute NaOH solution (0.3M) and renaturation by sequential dialysis, regenerated polysaccharide (RPD3) was obtained. The physico-chemical properties of RPD3 including intrinsic viscosity [η], molecular weight (Mw) and optical rotation were similar to those of PD3, which suggested that RPD3 also had a triple helical structure after denaturation–renaturation. However, different intrinsic viscosity dependence on the concentration of NaOH solution was noted in PD3 and RPD3, which indicated that RPD3 had lower chain tightness compared with PD3. The anti-tumor activity of this polysaccharide after denaturation–renaturation treatment was further investigated. Both PD3 and RPD3 showed no direct cytotoxicity against S-180 cells in vitro but behaved anti-tumor activity in vivo. 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| subjects | Animals Anti-tumor activity antineoplastic agents Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Antineoplastic Agents - toxicity Basidiomycota - chemistry Body Weight - drug effects Cell Line, Tumor Cytokines - blood Cytokines - metabolism cytotoxicity denaturation Denaturation–renaturation dialysis Dictyophora Dictyophora indusiata polysaccharide Fungal Polysaccharides - chemistry Fungal Polysaccharides - pharmacology Fungal Polysaccharides - toxicity Male Mice Molecular Weight Neoplasms - immunology Neoplasms - metabolism Neoplasms - pathology polysaccharides sodium hydroxide Viscosity |
| title | Anti-tumor activity of the regenerated triple-helical polysaccharide from Dictyophora indusiata |
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