Carbohydrate Binding and Unfolding of Spatholobus parviflorus Lectin: Fluorescence and Circular Dichroism Spectroscopic Study

Biophysical and carbohydrate binding studies have been carried out on a lectin of Spatholobus parviflorus (SPL) seeds isolated by affinity chromatography on cross-linked guar gum. It agglutinated erythrocytes of all ABO blood groups. SDS-PAGE, both in reducing and non-reducing conditions, showed two...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2013-09, Vol.171 (1), p.80-92
Hauptverfasser:	K., Geethanandan, Joseph, Abhilash, C., Sadasivan, Haridas, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillus Aspergillus niger - drug effects Binding sites Biochemistry Biological and medical sciences Biotechnology Carbohydrate Metabolism Chemistry Chemistry and Materials Science Circular Dichroism Erythrocytes Fabaceae - chemistry Fluorescence Fundamental and applied biological sciences. Psychology Fusarium - drug effects Guanidine - pharmacology Hemagglutination - drug effects Hydrogen-Ion Concentration Ligands Plant Lectins - chemistry Plant Lectins - isolation & purification Plant Lectins - metabolism Plant Lectins - pharmacology Protein Denaturation - drug effects Proteins Sequence Analysis Spectrometry, Fluorescence Temperature Urea Urea - pharmacology
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