Identifying HIF Activity in Three-Dimensional Cultures of Islet-Like Clusters

Purpose Hypoxia is a major cause for failure of encapsulated islet grafts. Three-dimensional (3D) re-aggregation and hypoxic preconditioning are used to help overcome this obstacle. However, it is still difficult to identify hypoxic cells in a 3D system. We evaluate the efficacy of a fluorescent sys...

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Veröffentlicht in:	International journal of artificial organs 2013-03, Vol.36 (3), p.175-183
Hauptverfasser:	Skiles, Matthew L., Wilder, Nathan B., Sahai, Suchit, Blanchette, James O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Hypoxia - physiology Cell Line, Tumor Cell Shape Hypoxia-Inducible Factor 1, alpha Subunit - metabolism Insulin-Secreting Cells - cytology Insulin-Secreting Cells - metabolism Insulinoma - metabolism Islets of Langerhans - cytology Islets of Langerhans - metabolism Mice Pancreatic Neoplasms - metabolism
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