Metabolic Behavior and Enzymatic Aspects of Denitrifying EBPR Sludge in a Continuous-Flow Anaerobic–Anoxic System
The metabolic aspects of enhanced biological phosphorus removal (EBPR) were investigated for the first time in a continuous-flow anaerobic–anoxic plant fed with acetate, propionate, or substrates which are involved in the tricarboxylic acid and/or glyoxylate cycle, i.e., fumarate, malate, or oxaloac...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2013-10, Vol.171 (4), p.939-953 |
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| creator | Zafiriadis, Ilias Ntougias, Spyridon Kapagiannidis, Anastasios G. Aivasidis, Alexander |
| description | The metabolic aspects of enhanced biological phosphorus removal (EBPR) were investigated for the first time in a continuous-flow anaerobic–anoxic plant fed with acetate, propionate, or substrates which are involved in the tricarboxylic acid and/or glyoxylate cycle, i.e., fumarate, malate, or oxaloacetate, as the sole carbon source. Although the polyphosphate-accumulating organisms (PAOs) population remained stable with any carbon source examined, no typical EBPR metabolism was observed during fumarate, malate, or oxaloacetate utilization. Specific enzymatic activities related to EBPR were determined in activated sludge homogenates and directly correlated with the nutrient metabolic rates. The experimental results indicated the direct involvement of alkaline phosphatase, pyrophosphatase, and exopolyphosphatase in the denitrifying EBPR process. Metabolic aspects of glyoxylate cycle enzymes are discussed with regard to the biomass anaerobic and anoxic activity. Process performance was highly influenced by the kind of substrate utilized, indicating that specific metabolic pathways should be followed to favor efficient EBPR. |
| doi_str_mv | 10.1007/s12010-013-0390-0 |
| format | Article |
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Although the polyphosphate-accumulating organisms (PAOs) population remained stable with any carbon source examined, no typical EBPR metabolism was observed during fumarate, malate, or oxaloacetate utilization. Specific enzymatic activities related to EBPR were determined in activated sludge homogenates and directly correlated with the nutrient metabolic rates. The experimental results indicated the direct involvement of alkaline phosphatase, pyrophosphatase, and exopolyphosphatase in the denitrifying EBPR process. Metabolic aspects of glyoxylate cycle enzymes are discussed with regard to the biomass anaerobic and anoxic activity. 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Although the polyphosphate-accumulating organisms (PAOs) population remained stable with any carbon source examined, no typical EBPR metabolism was observed during fumarate, malate, or oxaloacetate utilization. Specific enzymatic activities related to EBPR were determined in activated sludge homogenates and directly correlated with the nutrient metabolic rates. The experimental results indicated the direct involvement of alkaline phosphatase, pyrophosphatase, and exopolyphosphatase in the denitrifying EBPR process. Metabolic aspects of glyoxylate cycle enzymes are discussed with regard to the biomass anaerobic and anoxic activity. Process performance was highly influenced by the kind of substrate utilized, indicating that specific metabolic pathways should be followed to favor efficient EBPR.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23912208</pmid><doi>10.1007/s12010-013-0390-0</doi><tpages>15</tpages></addata></record> |
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| subjects | Acid Anhydride Hydrolases - metabolism Activated sludge Alkaline Phosphatase - metabolism Anaerobiosis Bacteria Biochemistry Biological and medical sciences Biological treatment of waters Biotechnology Carbon sources Chemistry Chemistry and Materials Science Denitrification Environment and pollution Enzymatic activity Fumarates - metabolism Fundamental and applied biological sciences. Psychology Industrial applications and implications. Economical aspects Malates - metabolism Oxaloacetic Acid - metabolism Phosphoric Monoester Hydrolases - metabolism Phosphorus removal Polyphosphates - metabolism Pyrophosphatases - metabolism Sewage - microbiology Sludge Water treatment |
| title | Metabolic Behavior and Enzymatic Aspects of Denitrifying EBPR Sludge in a Continuous-Flow Anaerobic–Anoxic System |
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