behavior of the plasma membrane following osmotic contraction of isolated protoplasts: implications in freezing injury

Following osmotic contraction of isolated rye protoplast (Secale cereale L. cv. Puma) that results in nearly a 50% reduction in volume, the plasma membrane was smooth, with no folding or pleating. Instead, deletion of plasma membrane occurred and numerous cytoplasmic vesicles were observed. As a res...

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Veröffentlicht in:	Protoplasma 1984-06, Vol.123 (2), p.83-94
Hauptverfasser:	Gordon-Kamm, W.J, Steponkus, P.L
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences freezing Fundamental and applied biological sciences. Psychology ice damage membranes osmotic stress Plant physiology and development protoplasts rye Secale cereale Tissue cultures, protoplasts
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description	Following osmotic contraction of isolated rye protoplast (Secale cereale L. cv. Puma) that results in nearly a 50% reduction in volume, the plasma membrane was smooth, with no folding or pleating. Instead, deletion of plasma membrane occurred and numerous cytoplasmic vesicles were observed. As a result, the area of the plasma membrane was reduced by approximately 40%. Thin sections revealed that the cytoplasmic vesicles were membrane bound and not merely voids in the cytoplasm. The results using osmotic manipulation confirm earlier observations of isolated protoplasts at the light microscope level. Upon contraction plasma membrane is deleted into cytoplasmic vesicle, which are not readily reincorporated into the plasma membrane upon expansion. Lysis occurs before the original volume and surface area are regained.
doi_str_mv	10.1007/bf01283579
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Psychology</subject><subject>ice damage</subject><subject>membranes</subject><subject>osmotic stress</subject><subject>Plant physiology and development</subject><subject>protoplasts</subject><subject>rye</subject><subject>Secale cereale</subject><subject>Tissue cultures, protoplasts</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNo90E9PFTEUBfDGaOIT3fgF6IKwIBm9baedDjsggiYkLpTE3eRO_0BJZ_po-yD46ZnJQ1fd_M7J7SHkM4MvDKD7OnpgXAvZ9W_IhikmG8WAvyUbACEapsWf9-RDKfcAIDnIDXkc3R0-hpRp8rTeObqNWCakk5vGjLOjPsWYnsJ8S1OZUg2GmjTXjKaGNK-hUFLE6izd5lTTGq_llIZpG4PBFRUaZuqzc3_XljDf7_LzR_LOYyzu0-t7QG4uv_2--N5c_7z6cXF23RjR9bVpBRot3diDHn3rbGtBetEqJay1qFBz3fdSCHAtKmnRS8NGNEx33HJwVhyQ433vctzDzpU6TKEYF-PytbQrA2tbrmQnFniyhyanUrLzwzaHCfPzwGBYpx3OL_9Nu-Cj11YsBqNfhjKh_E_00GvF-cIO98xjGvA2L-TmFwcmgCuh216LF38FhNg</recordid><startdate>198406</startdate><enddate>198406</enddate><creator>Gordon-Kamm, W.J</creator><creator>Steponkus, P.L</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>198406</creationdate><title>behavior of the plasma membrane following osmotic contraction of isolated protoplasts: implications in freezing injury</title><author>Gordon-Kamm, W.J ; Steponkus, P.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-43ac85eb908bf4ed4d05f34663ddda6a828995330e4a65daf5c1bac1872d20ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Biological and medical sciences</topic><topic>freezing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>ice damage</topic><topic>membranes</topic><topic>osmotic stress</topic><topic>Plant physiology and development</topic><topic>protoplasts</topic><topic>rye</topic><topic>Secale cereale</topic><topic>Tissue cultures, protoplasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gordon-Kamm, W.J</creatorcontrib><creatorcontrib>Steponkus, P.L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Protoplasma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gordon-Kamm, W.J</au><au>Steponkus, P.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>behavior of the plasma membrane following osmotic contraction of isolated protoplasts: implications in freezing injury</atitle><jtitle>Protoplasma</jtitle><date>1984-06</date><risdate>1984</risdate><volume>123</volume><issue>2</issue><spage>83</spage><epage>94</epage><pages>83-94</pages><issn>0033-183X</issn><eissn>1615-6102</eissn><coden>PROTA5</coden><abstract>Following osmotic contraction of isolated rye protoplast (Secale cereale L. cv. 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subjects	Biological and medical sciences freezing Fundamental and applied biological sciences. Psychology ice damage membranes osmotic stress Plant physiology and development protoplasts rye Secale cereale Tissue cultures, protoplasts
title	behavior of the plasma membrane following osmotic contraction of isolated protoplasts: implications in freezing injury
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