Protoplasts from Podospora anserina: isolation, purification, and transformation

Protoplasts from Podospora anserina mycelium were produced using the commercially available enzyme Novozym 234. Different parameters involved in protoplast isolation were analyzed to establish optimal conditions, and protoplast production was notably increased. For the purification of protoplasts, s...

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Veröffentlicht in:	Current microbiology 1985-01, Vol.12 (5), p.301-306
Hauptverfasser:	Ferrer, S, Ramon, D, Salom, J, Vicente, E, Uruburu, F
Format:	Artikel
Sprache:	eng
Schlagworte:	AISLAMIENTO (TECNICA) CHAMPIGNON enzymes EPURATION FUNGI ISOLATION ISOLEMENT Podospora anserina PROTOPLASM PROTOPLASMA PROTOPLASME protoplasts PURIFICACION PURIFICATION REGENERACION REGENERATION transformation
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