Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy) sub(3) super(2+)-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers
An electrogenerated chemiluminescence (ECL)-based immunosensor for the detection of immunoglobulin G (IgG) has been fabricated using Ru(bpy) sub(3) super(2+)-doped silica nanoparticles and calix[4]crown-5 self-assembled monolayers (SAMs). Ru(bpy) sub(3) super(2+)-doped silica nanoparticles are prepa...
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| Veröffentlicht in: | Electroanalysis (New York, N.Y.) N.Y.), 2013-04, Vol.25 (4), p.1056-1063 |
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| container_title | Electroanalysis (New York, N.Y.) |
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| creator | Kim, Yang-Rae Seo, Hyo-Ju Oh, Jeong-Wook Lim, Hyunchang Kim, Tae Hyun Kim, Hasuck |
| description | An electrogenerated chemiluminescence (ECL)-based immunosensor for the detection of immunoglobulin G (IgG) has been fabricated using Ru(bpy) sub(3) super(2+)-doped silica nanoparticles and calix[4]crown-5 self-assembled monolayers (SAMs). Ru(bpy) sub(3) super(2+)-doped silica nanoparticles are prepared by the water-in-oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calix[4]crown-5 derivative, is utilized for the immobilization of anti-immunoglobulin G (Anti-IgG) on a gold electrode. The concentration of IgG is measured using a sandwich-type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 5-30 mu gmL super(-1). The detection limit of IgG is 1.5 mu gmL super(-1). |
| doi_str_mv | 10.1002/elan.201200548 |
| format | Article |
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Ru(bpy) sub(3) super(2+)-doped silica nanoparticles are prepared by the water-in-oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calix[4]crown-5 derivative, is utilized for the immobilization of anti-immunoglobulin G (Anti-IgG) on a gold electrode. The concentration of IgG is measured using a sandwich-type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 5-30 mu gmL super(-1). 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Ru(bpy) sub(3) super(2+)-doped silica nanoparticles are prepared by the water-in-oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calix[4]crown-5 derivative, is utilized for the immobilization of anti-immunoglobulin G (Anti-IgG) on a gold electrode. The concentration of IgG is measured using a sandwich-type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 5-30 mu gmL super(-1). The detection limit of IgG is 1.5 mu gmL super(-1).</description><subject>Chemiluminescence</subject><subject>Derivatives</subject><subject>Electrodes</subject><subject>Immobilization</subject><subject>Immunosensors</subject><subject>Nanoparticles</subject><subject>Self-assembled monolayers</subject><subject>Silicon dioxide</subject><issn>1040-0397</issn><issn>1521-4109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqVT0tOwzAUtBBIlM-WtZepkMtz4lCyhLYIFrCgsEKocsJrMfIn-MWC3oajYiQuwGpGo5nRDGMnEiYSoDxDq_2kBFkC1Opih41kXUqhJDS7mYMCAVUz3WcHRO8A0JyrZsS-b51LPhB6CpFfacJXHjxfWOyGGDboMeoha7M3dMYmZzxSh75D_kTGb_hDKtp-O-aU2qL6hR5jUZ6OxTz0ObY01nSa32sfeh0H01kkrn3u09Z8PauXLoZPL2q-RLsWl0ToWptzd8EHq7cY6YjtrbUlPP7DQ1ZcLx5nN6KP4SMhDStn8iKbz2NItJIqn5zWFajqH9Yfq_llwA</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Kim, Yang-Rae</creator><creator>Seo, Hyo-Ju</creator><creator>Oh, Jeong-Wook</creator><creator>Lim, Hyunchang</creator><creator>Kim, Tae Hyun</creator><creator>Kim, Hasuck</creator><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20130401</creationdate><title>Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy) sub(3) super(2+)-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers</title><author>Kim, Yang-Rae ; Seo, Hyo-Ju ; Oh, Jeong-Wook ; Lim, Hyunchang ; Kim, Tae Hyun ; Kim, Hasuck</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_14397753043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Chemiluminescence</topic><topic>Derivatives</topic><topic>Electrodes</topic><topic>Immobilization</topic><topic>Immunosensors</topic><topic>Nanoparticles</topic><topic>Self-assembled monolayers</topic><topic>Silicon dioxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yang-Rae</creatorcontrib><creatorcontrib>Seo, Hyo-Ju</creatorcontrib><creatorcontrib>Oh, Jeong-Wook</creatorcontrib><creatorcontrib>Lim, Hyunchang</creatorcontrib><creatorcontrib>Kim, Tae Hyun</creatorcontrib><creatorcontrib>Kim, Hasuck</creatorcontrib><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Electroanalysis (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yang-Rae</au><au>Seo, Hyo-Ju</au><au>Oh, Jeong-Wook</au><au>Lim, Hyunchang</au><au>Kim, Tae Hyun</au><au>Kim, Hasuck</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy) sub(3) super(2+)-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers</atitle><jtitle>Electroanalysis (New York, N.Y.)</jtitle><date>2013-04-01</date><risdate>2013</risdate><volume>25</volume><issue>4</issue><spage>1056</spage><epage>1063</epage><pages>1056-1063</pages><issn>1040-0397</issn><eissn>1521-4109</eissn><abstract>An electrogenerated chemiluminescence (ECL)-based immunosensor for the detection of immunoglobulin G (IgG) has been fabricated using Ru(bpy) sub(3) super(2+)-doped silica nanoparticles and calix[4]crown-5 self-assembled monolayers (SAMs). Ru(bpy) sub(3) super(2+)-doped silica nanoparticles are prepared by the water-in-oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calix[4]crown-5 derivative, is utilized for the immobilization of anti-immunoglobulin G (Anti-IgG) on a gold electrode. The concentration of IgG is measured using a sandwich-type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 5-30 mu gmL super(-1). The detection limit of IgG is 1.5 mu gmL super(-1).</abstract><doi>10.1002/elan.201200548</doi></addata></record> |
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| ispartof | Electroanalysis (New York, N.Y.), 2013-04, Vol.25 (4), p.1056-1063 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Chemiluminescence Derivatives Electrodes Immobilization Immunosensors Nanoparticles Self-assembled monolayers Silicon dioxide |
| title | Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy) sub(3) super(2+)-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers |
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