Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry

A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), r...

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Veröffentlicht in:Journal of separation science 2013-06, Vol.36 (12), p.1953-1958
Hauptverfasser: Guo, Na, Yang, Dawei, Ablajan, Keyume, Niu, Xiaohong, Fan, Bin, Wang, Zhiguo, Dai, Jingang, Wu, Xiaoxia, Liu, Baidong
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container_end_page 1958
container_issue 12
container_start_page 1953
container_title Journal of separation science
container_volume 36
creator Guo, Na
Yang, Dawei
Ablajan, Keyume
Niu, Xiaohong
Fan, Bin
Wang, Zhiguo
Dai, Jingang
Wu, Xiaoxia
Liu, Baidong
description A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra‐ and interday precisions (RSD < 5.07%) with good accuracies (97.76–105.08%) and good recovery (95.0–107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30–17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.
doi_str_mv 10.1002/jssc.201300012
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The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r &gt; 0.9995), repeatability (RSD &lt; 4.36%), intra‐ and interday precisions (RSD &lt; 5.07%) with good accuracies (97.76–105.08%) and good recovery (95.0–107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30–17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. 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Sep. Science</addtitle><description>A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r &gt; 0.9995), repeatability (RSD &lt; 4.36%), intra‐ and interday precisions (RSD &lt; 5.07%) with good accuracies (97.76–105.08%) and good recovery (95.0–107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30–17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. 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Drug treatments</topic><topic>Quantitative analysis</topic><topic>Rapid resolution LC</topic><topic>Repeatability</topic><topic>Reproducibility</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Na</creatorcontrib><creatorcontrib>Yang, Dawei</creatorcontrib><creatorcontrib>Ablajan, Keyume</creatorcontrib><creatorcontrib>Niu, Xiaohong</creatorcontrib><creatorcontrib>Fan, Bin</creatorcontrib><creatorcontrib>Wang, Zhiguo</creatorcontrib><creatorcontrib>Dai, Jingang</creatorcontrib><creatorcontrib>Wu, Xiaoxia</creatorcontrib><creatorcontrib>Liu, Baidong</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Na</au><au>Yang, Dawei</au><au>Ablajan, Keyume</au><au>Niu, Xiaohong</au><au>Fan, Bin</au><au>Wang, Zhiguo</au><au>Dai, Jingang</au><au>Wu, Xiaoxia</au><au>Liu, Baidong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2013-06</date><risdate>2013</risdate><volume>36</volume><issue>12</issue><spage>1953</spage><epage>1958</epage><pages>1953-1958</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r &gt; 0.9995), repeatability (RSD &lt; 4.36%), intra‐ and interday precisions (RSD &lt; 5.07%) with good accuracies (97.76–105.08%) and good recovery (95.0–107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30–17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.</abstract><cop>Weinheim</cop><pub>Blackwell Publishing Ltd</pub><pmid>23576380</pmid><doi>10.1002/jssc.201300012</doi><tpages>6</tpages></addata></record>
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source MEDLINE; Wiley Journals
subjects Aconitum alkaloids
Alkaloids
Alkaloids - chemistry
Assessments
Baifupian
Biological and medical sciences
Chromatography
Chromatography, High Pressure Liquid - methods
Drugs, Chinese Herbal - chemistry
Fuzi
General pharmacology
Heishunpian
Markers
Mass spectrometry
Mathematical analysis
Medical sciences
Molecular chemistry
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Quantitative analysis
Rapid resolution LC
Repeatability
Reproducibility
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
title Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry
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