Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry
A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), r...
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Veröffentlicht in: | Journal of separation science 2013-06, Vol.36 (12), p.1953-1958 |
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container_end_page | 1958 |
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container_issue | 12 |
container_start_page | 1953 |
container_title | Journal of separation science |
container_volume | 36 |
creator | Guo, Na Yang, Dawei Ablajan, Keyume Niu, Xiaohong Fan, Bin Wang, Zhiguo Dai, Jingang Wu, Xiaoxia Liu, Baidong |
description | A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra‐ and interday precisions (RSD < 5.07%) with good accuracies (97.76–105.08%) and good recovery (95.0–107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30–17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction. |
doi_str_mv | 10.1002/jssc.201300012 |
format | Article |
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The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra‐ and interday precisions (RSD < 5.07%) with good accuracies (97.76–105.08%) and good recovery (95.0–107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30–17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.201300012</identifier><identifier>PMID: 23576380</identifier><language>eng</language><publisher>Weinheim: Blackwell Publishing Ltd</publisher><subject>Aconitum alkaloids ; Alkaloids ; Alkaloids - chemistry ; Assessments ; Baifupian ; Biological and medical sciences ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Drugs, Chinese Herbal - chemistry ; Fuzi ; General pharmacology ; Heishunpian ; Markers ; Mass spectrometry ; Mathematical analysis ; Medical sciences ; Molecular chemistry ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Quantitative analysis ; Rapid resolution LC ; Repeatability ; Reproducibility ; Spectrometry, Mass, Electrospray Ionization - methods ; Tandem Mass Spectrometry - methods</subject><ispartof>Journal of separation science, 2013-06, Vol.36 (12), p.1953-1958</ispartof><rights>2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2014 INIST-CNRS</rights><rights>2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4694-522b0766f7408a53430c81613cdb69196ff18a75712d6ebdf55044050b25b9873</citedby><cites>FETCH-LOGICAL-c4694-522b0766f7408a53430c81613cdb69196ff18a75712d6ebdf55044050b25b9873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.201300012$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.201300012$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27465884$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23576380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Na</creatorcontrib><creatorcontrib>Yang, Dawei</creatorcontrib><creatorcontrib>Ablajan, Keyume</creatorcontrib><creatorcontrib>Niu, Xiaohong</creatorcontrib><creatorcontrib>Fan, Bin</creatorcontrib><creatorcontrib>Wang, Zhiguo</creatorcontrib><creatorcontrib>Dai, Jingang</creatorcontrib><creatorcontrib>Wu, Xiaoxia</creatorcontrib><creatorcontrib>Liu, Baidong</creatorcontrib><title>Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry</title><title>Journal of separation science</title><addtitle>J. Sep. Science</addtitle><description>A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra‐ and interday precisions (RSD < 5.07%) with good accuracies (97.76–105.08%) and good recovery (95.0–107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30–17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.</description><subject>Aconitum alkaloids</subject><subject>Alkaloids</subject><subject>Alkaloids - chemistry</subject><subject>Assessments</subject><subject>Baifupian</subject><subject>Biological and medical sciences</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Drugs, Chinese Herbal - chemistry</subject><subject>Fuzi</subject><subject>General pharmacology</subject><subject>Heishunpian</subject><subject>Markers</subject><subject>Mass spectrometry</subject><subject>Mathematical analysis</subject><subject>Medical sciences</subject><subject>Molecular chemistry</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Quantitative analysis</subject><subject>Rapid resolution LC</subject><subject>Repeatability</subject><subject>Reproducibility</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0UuP0zAQB_AIgdgHXDkiSwiJS4vfTo6osMtjeUhd4Gg5jkPddeLWk-xSPgkfF29bisQFTras38xo_C-KRwRPCcb0-RLATikmDGNM6J3imEgiJhUj_O7hjuVRcQKwzEKVFb5fHFEmlGQlPi5-zn03hsH0Lo6A1qPpBz-YwccexRaBu3Y9MuHKhOgbQL5HqxStA3ANOht_eNQ4G-2W1xuUzMo3KDmIYdy-Bb8e84tdpNiZIX7LYLFBNo6rkBvc-GGB8ujGdagzAAhWzg6ZuiFtHhT3WhPAPdyfp8Xns1eXs9eTi4_nb2YvLiaWy4pPBKU1VlK2iuPSCMYZtmXem9mmlhWpZNuS0iihCG2kq5tWCMw5Frimoq5KxU6LZ7u-ebH16GDQnQfrQth9iSacVUrxkvwHZbJSRFFZZfrkL7qMY-rzItuGFRUMi6ymO2VTBEiu1avkO5M2mmB9G6--jVcf4s0Fj_dtx7pzzYH_zjODp3tgwJrQJtNbD3-c4lKUJc-O79yND27zj7H67Xw-Y3RbNtmVeRjc90OZSVdaKqaE_vrhXNNPX15evpfvtGK_ABR0zqo</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Guo, Na</creator><creator>Yang, Dawei</creator><creator>Ablajan, Keyume</creator><creator>Niu, Xiaohong</creator><creator>Fan, Bin</creator><creator>Wang, Zhiguo</creator><creator>Dai, Jingang</creator><creator>Wu, Xiaoxia</creator><creator>Liu, Baidong</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>201306</creationdate><title>Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry</title><author>Guo, Na ; Yang, Dawei ; Ablajan, Keyume ; Niu, Xiaohong ; Fan, Bin ; Wang, Zhiguo ; Dai, Jingang ; Wu, Xiaoxia ; Liu, Baidong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4694-522b0766f7408a53430c81613cdb69196ff18a75712d6ebdf55044050b25b9873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aconitum alkaloids</topic><topic>Alkaloids</topic><topic>Alkaloids - chemistry</topic><topic>Assessments</topic><topic>Baifupian</topic><topic>Biological and medical sciences</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Drugs, Chinese Herbal - chemistry</topic><topic>Fuzi</topic><topic>General pharmacology</topic><topic>Heishunpian</topic><topic>Markers</topic><topic>Mass spectrometry</topic><topic>Mathematical analysis</topic><topic>Medical sciences</topic><topic>Molecular chemistry</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Quantitative analysis</topic><topic>Rapid resolution LC</topic><topic>Repeatability</topic><topic>Reproducibility</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Na</creatorcontrib><creatorcontrib>Yang, Dawei</creatorcontrib><creatorcontrib>Ablajan, Keyume</creatorcontrib><creatorcontrib>Niu, Xiaohong</creatorcontrib><creatorcontrib>Fan, Bin</creatorcontrib><creatorcontrib>Wang, Zhiguo</creatorcontrib><creatorcontrib>Dai, Jingang</creatorcontrib><creatorcontrib>Wu, Xiaoxia</creatorcontrib><creatorcontrib>Liu, Baidong</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Na</au><au>Yang, Dawei</au><au>Ablajan, Keyume</au><au>Niu, Xiaohong</au><au>Fan, Bin</au><au>Wang, Zhiguo</au><au>Dai, Jingang</au><au>Wu, Xiaoxia</au><au>Liu, Baidong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2013-06</date><risdate>2013</risdate><volume>36</volume><issue>12</issue><spage>1953</spage><epage>1958</epage><pages>1953-1958</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra‐ and interday precisions (RSD < 5.07%) with good accuracies (97.76–105.08%) and good recovery (95.0–107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30–17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.</abstract><cop>Weinheim</cop><pub>Blackwell Publishing Ltd</pub><pmid>23576380</pmid><doi>10.1002/jssc.201300012</doi><tpages>6</tpages></addata></record> |
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subjects | Aconitum alkaloids Alkaloids Alkaloids - chemistry Assessments Baifupian Biological and medical sciences Chromatography Chromatography, High Pressure Liquid - methods Drugs, Chinese Herbal - chemistry Fuzi General pharmacology Heishunpian Markers Mass spectrometry Mathematical analysis Medical sciences Molecular chemistry Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Quantitative analysis Rapid resolution LC Repeatability Reproducibility Spectrometry, Mass, Electrospray Ionization - methods Tandem Mass Spectrometry - methods |
title | Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry |
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