Heterologous expression of CYP102A5 variant from Bacillus cereus CYPPB-1: Validation of model for predicting drug metabolism of human P450 probe substrates

CYP102A5 variant (ADL27534) from isolated Bacillus cereus CYPPB-1 was heterologously expressed in Escherichia coli Top 10 cells. Comparative sequence analysis of purified CYP102A5 variant with respect to reported CYP102A5 (AAP10153) from Bacillus cereus ATCC 14579 revealed amino acid sequence change...

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Veröffentlicht in:	Applied microbiology and biotechnology 2013-09, Vol.97 (18), p.8107-8119
Hauptverfasser:	Pendyala, Brahmaiah, Chaganti, Subba Rao, Thadikamala, Sathish, Reddy Shetty, Prakasham
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Analysis Aryl Hydrocarbon Hydroxylases - metabolism Bacillus cereus Bacillus cereus - chemistry Bacillus cereus - enzymology Bacillus cereus - genetics Bacteria Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Biomedical and Life Sciences Biosynthesis Biotechnologically Relevant Enzymes and Proteins Biotechnology Cloning Cytochrome Cytochrome P-450 Cytochrome P-450 CYP2C9 Cytochrome P-450 CYP2E1 - genetics Cytochrome P-450 CYP2E1 - metabolism Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism E coli Enzymes Escherichia coli Gene Expression Humans Inactivation, Metabolic Kinetics Life Sciences Metabolism Metabolites Microbial Genetics and Genomics Microbiology Molecular Docking Simulation Oxidation Physiological aspects Polypeptides Studies Substrate Specificity Substrates
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