Large-scale preparation of Shiga toxin 2 in Escherichia coli for toxoid vaccine antigen production

Enterohemorrhagic Escherichia coli (EHEC) causes hemorrhagic colitis, and in more severe cases, a serious clinical complication called hemolytic uremic syndrome (HUS). Shiga toxin (Stx)is one of the factors that cause HUS. Serotypes of Stx produced by EHEC include Stx1 and Stx2. Although some geneti...

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Veröffentlicht in:	Microbiology and immunology 2013-01, Vol.57 (1), p.38-45
Hauptverfasser:	Arimitsu, Hideyuki, Sasaki, Keiko, Shimizu, Takeshi, Tsukamoto, Kentaro, Shimizu, Toshiyasu, Tsuji, Takao
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bacterial - blood Antibodies, Neutralizing - blood Antigens, Bacterial - biosynthesis Antigens, Bacterial - genetics Antigens, Bacterial - immunology Antigens, Bacterial - toxicity Bacterial Vaccines - isolation & purification Bacteriology Cell Survival - drug effects Chromatography, Affinity Chromatography, Liquid E coli Escherichia coli Escherichia coli - genetics Female Gene Expression Genetic Vectors HeLa Cells Humans large-scale expression lincomycin Mice Mice, Inbred ICR Plasmids Poisoning Shiga toxin 2 Shiga Toxin 2 - biosynthesis Shiga Toxin 2 - genetics Shiga Toxin 2 - isolation & purification Shiga Toxin 2 - toxicity Survival Analysis Toxoids - isolation & purification Toxoids - toxicity vaccine Vaccines Vaccines, Synthetic - isolation & purification
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