Qiagen's Investigator™ Quantiplex Kit as a Predictor of STR Amplification Success from Low-Yield DNA Samples
Qiagen's Investigator™ Quantiplex kit, a total human DNA quantitation kit, has a 200‐base pair internal control, fast cycling time, and scorpion molecules containing a covalently linked primer, probe, fluorophore, and quencher. The Investigator™ Quantiplex kit was evaluated to investigate a val...
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| Veröffentlicht in: | Journal of forensic sciences 2013-09, Vol.58 (5), p.1306-1309 |
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| creator | Thomas, Jacqueline T. Berlin, Rebecca M. Barker, Jessica M. Dawson Cruz, Tracey |
| description | Qiagen's Investigator™ Quantiplex kit, a total human DNA quantitation kit, has a 200‐base pair internal control, fast cycling time, and scorpion molecules containing a covalently linked primer, probe, fluorophore, and quencher. The Investigator™ Quantiplex kit was evaluated to investigate a value under which complete short tandem repeat (STR) failure was consistently obtained. Buccal swabs were extracted using the Qiagen QIAamp® DNA Blood Mini Kit, quantified with the Investigator™ Quantiplex kit using a tested half‐volume reaction, amplified with the ABI AmpFlSTR® Identifiler kit, separated on the 3100Avant Genetic Analyzer, and data analyzed with GeneMapper® ID v.3.2. While undetected samples were unlikely to produce sufficient data for statistical calculations or CODIS upload (2.00 alleles and 0.82 complete loci on average), data may be useful for exclusionary purposes. Thus, the Investigator™ Quantiplex kit may be useful for predicting STR success. These findings are comparable with previously reported data from the Quantifiler™ Human kit. |
| doi_str_mv | 10.1111/1556-4029.12171 |
| format | Article |
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The Investigator™ Quantiplex kit was evaluated to investigate a value under which complete short tandem repeat (STR) failure was consistently obtained. Buccal swabs were extracted using the Qiagen QIAamp® DNA Blood Mini Kit, quantified with the Investigator™ Quantiplex kit using a tested half‐volume reaction, amplified with the ABI AmpFlSTR® Identifiler kit, separated on the 3100Avant Genetic Analyzer, and data analyzed with GeneMapper® ID v.3.2. While undetected samples were unlikely to produce sufficient data for statistical calculations or CODIS upload (2.00 alleles and 0.82 complete loci on average), data may be useful for exclusionary purposes. Thus, the Investigator™ Quantiplex kit may be useful for predicting STR success. These findings are comparable with previously reported data from the Quantifiler™ Human kit.</description><identifier>ISSN: 0022-1198</identifier><identifier>EISSN: 1556-4029</identifier><identifier>DOI: 10.1111/1556-4029.12171</identifier><identifier>PMID: 23786239</identifier><identifier>CODEN: JFSCAS</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Amplification ; Blood ; Deoxyribonucleic acid ; DNA ; DNA - analysis ; DNA Fingerprinting - instrumentation ; DNA quantitation ; forensic biology ; forensic science ; Forensic sciences ; Humans ; Investigator Quantiplex ; Microsatellite Repeats ; Molecules ; quantitative polymerase chain reaction ; Real-Time Polymerase Chain Reaction - instrumentation ; short tandem repeat success</subject><ispartof>Journal of forensic sciences, 2013-09, Vol.58 (5), p.1306-1309</ispartof><rights>2013 American Academy of Forensic Sciences</rights><rights>2013 American Academy of Forensic Sciences.</rights><rights>Copyright American Society for Testing and Materials Sep 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4701-e6101c78c6da7d52a4690c375727126c42179365643af2b348e9fd294c7e5ea53</citedby><cites>FETCH-LOGICAL-c4701-e6101c78c6da7d52a4690c375727126c42179365643af2b348e9fd294c7e5ea53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1556-4029.12171$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1556-4029.12171$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23786239$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomas, Jacqueline T.</creatorcontrib><creatorcontrib>Berlin, Rebecca M.</creatorcontrib><creatorcontrib>Barker, Jessica M.</creatorcontrib><creatorcontrib>Dawson Cruz, Tracey</creatorcontrib><title>Qiagen's Investigator™ Quantiplex Kit as a Predictor of STR Amplification Success from Low-Yield DNA Samples</title><title>Journal of forensic sciences</title><addtitle>J Forensic Sci</addtitle><description>Qiagen's Investigator™ Quantiplex kit, a total human DNA quantitation kit, has a 200‐base pair internal control, fast cycling time, and scorpion molecules containing a covalently linked primer, probe, fluorophore, and quencher. The Investigator™ Quantiplex kit was evaluated to investigate a value under which complete short tandem repeat (STR) failure was consistently obtained. Buccal swabs were extracted using the Qiagen QIAamp® DNA Blood Mini Kit, quantified with the Investigator™ Quantiplex kit using a tested half‐volume reaction, amplified with the ABI AmpFlSTR® Identifiler kit, separated on the 3100Avant Genetic Analyzer, and data analyzed with GeneMapper® ID v.3.2. While undetected samples were unlikely to produce sufficient data for statistical calculations or CODIS upload (2.00 alleles and 0.82 complete loci on average), data may be useful for exclusionary purposes. Thus, the Investigator™ Quantiplex kit may be useful for predicting STR success. These findings are comparable with previously reported data from the Quantifiler™ Human kit.</description><subject>Amplification</subject><subject>Blood</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA Fingerprinting - instrumentation</subject><subject>DNA quantitation</subject><subject>forensic biology</subject><subject>forensic science</subject><subject>Forensic sciences</subject><subject>Humans</subject><subject>Investigator Quantiplex</subject><subject>Microsatellite Repeats</subject><subject>Molecules</subject><subject>quantitative polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction - instrumentation</subject><subject>short tandem repeat success</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokNhzQ5ZYgGbtP6J7Xg5TGkpjFrKFCpWluvcVC5JPNgJbfc8CY_Gk-Bh2lmwAO7G0tV3ju7xQegpJTs0zy4VQhYlYXqHMqroPTTZbO6jCSGMFZTqags9SumSECKppA_RFuOqkozrCepPvL2A_kXCh_03SIO_sEOIP7__wCej7Qe_bOEav_MDtglb_D5C7V0GcGjw4vQDnnbL1jfe2cGHHi9G5yAl3MTQ4Xm4Kj57aGu8dzTFC5tJSI_Rg8a2CZ7cvtvo4_7r09mbYn58cDibzgtXKkILkJRQpyona6tqwWwpNXFcCcUUZdKVOazmUsiS24ad87IC3dRMl06BACv4Nnq59l3G8HXMuUznk4O2tT2EMRlacs2Y1lL-D0oqSYigGX3-B3oZxtjnIIapSudrhCR_o7IXY0QRsbpwd025GFKK0Jhl9J2NN4YSs-rWrJo0qybN726z4tmt73jeQb3h78rMgFgDV76Fm3_5mbf7x3fGxVrn0wDXG52NX4xU-dPN2dGBmbGq2lucvTKf-C-un7nE</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Thomas, Jacqueline T.</creator><creator>Berlin, Rebecca M.</creator><creator>Barker, Jessica M.</creator><creator>Dawson Cruz, Tracey</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>201309</creationdate><title>Qiagen's Investigator™ Quantiplex Kit as a Predictor of STR Amplification Success from Low-Yield DNA Samples</title><author>Thomas, Jacqueline T. ; Berlin, Rebecca M. ; Barker, Jessica M. ; Dawson Cruz, Tracey</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4701-e6101c78c6da7d52a4690c375727126c42179365643af2b348e9fd294c7e5ea53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amplification</topic><topic>Blood</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA Fingerprinting - instrumentation</topic><topic>DNA quantitation</topic><topic>forensic biology</topic><topic>forensic science</topic><topic>Forensic sciences</topic><topic>Humans</topic><topic>Investigator Quantiplex</topic><topic>Microsatellite Repeats</topic><topic>Molecules</topic><topic>quantitative polymerase chain reaction</topic><topic>Real-Time Polymerase Chain Reaction - instrumentation</topic><topic>short tandem repeat success</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomas, Jacqueline T.</creatorcontrib><creatorcontrib>Berlin, Rebecca M.</creatorcontrib><creatorcontrib>Barker, Jessica M.</creatorcontrib><creatorcontrib>Dawson Cruz, Tracey</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomas, Jacqueline T.</au><au>Berlin, Rebecca M.</au><au>Barker, Jessica M.</au><au>Dawson Cruz, Tracey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Qiagen's Investigator™ Quantiplex Kit as a Predictor of STR Amplification Success from Low-Yield DNA Samples</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2013-09</date><risdate>2013</risdate><volume>58</volume><issue>5</issue><spage>1306</spage><epage>1309</epage><pages>1306-1309</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><coden>JFSCAS</coden><abstract>Qiagen's Investigator™ Quantiplex kit, a total human DNA quantitation kit, has a 200‐base pair internal control, fast cycling time, and scorpion molecules containing a covalently linked primer, probe, fluorophore, and quencher. The Investigator™ Quantiplex kit was evaluated to investigate a value under which complete short tandem repeat (STR) failure was consistently obtained. Buccal swabs were extracted using the Qiagen QIAamp® DNA Blood Mini Kit, quantified with the Investigator™ Quantiplex kit using a tested half‐volume reaction, amplified with the ABI AmpFlSTR® Identifiler kit, separated on the 3100Avant Genetic Analyzer, and data analyzed with GeneMapper® ID v.3.2. While undetected samples were unlikely to produce sufficient data for statistical calculations or CODIS upload (2.00 alleles and 0.82 complete loci on average), data may be useful for exclusionary purposes. Thus, the Investigator™ Quantiplex kit may be useful for predicting STR success. These findings are comparable with previously reported data from the Quantifiler™ Human kit.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>23786239</pmid><doi>10.1111/1556-4029.12171</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1198 |
| ispartof | Journal of forensic sciences, 2013-09, Vol.58 (5), p.1306-1309 |
| issn | 0022-1198 1556-4029 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1439229966 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amplification Blood Deoxyribonucleic acid DNA DNA - analysis DNA Fingerprinting - instrumentation DNA quantitation forensic biology forensic science Forensic sciences Humans Investigator Quantiplex Microsatellite Repeats Molecules quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction - instrumentation short tandem repeat success |
| title | Qiagen's Investigator™ Quantiplex Kit as a Predictor of STR Amplification Success from Low-Yield DNA Samples |
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