Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica
Abstract A total of 71 isolates were collected from lake sediment and soil surrounding lakes in the Skarvsnes area, Antarctica. Based on ITS region sequence similarity, these isolates were classified to 10 genera. Twenty-three isolates were categorized as ascomycetous fungi from five genera (Embelli...
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| Veröffentlicht in: | FEMS microbiology letters 2013-09, Vol.346 (2), p.121-130 |
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| creator | Tsuji, Masaharu Fujiu, Seiichi Xiao, Nan Hanada, Yuichi Kudoh, Sakae Kondo, Hidemasa Tsuda, Sakae Hoshino, Tamotsu |
| description | Abstract
A total of 71 isolates were collected from lake sediment and soil surrounding lakes in the Skarvsnes area, Antarctica. Based on ITS region sequence similarity, these isolates were classified to 10 genera. Twenty-three isolates were categorized as ascomycetous fungi from five genera (Embellisia, Phoma, Geomyces, Tetracladium or Thelebolus) and 48 isolates were categorized as basidiomycetous fungi in five genera (Mrakia, Cryptococcus, Dioszegia, Rhodotorula or Leucosporidium). Thirty-five percent of culturable fungi were of the genus Mrakia. Eighteen isolates from eight genera were selected and tested for both antifreeze activity and capacity for growth under temperatures ranging from −1 to 25 °C. Rhodotorula sp. NHT-2 possessed a high degree of sequence homology with R. gracialis, while Leucosporidium sp. BSS-1 possessed a high degree of sequence homology with Leu. antarcticum (Glaciozyma antarctica), and these two isolates demonstrated antifreeze activity. All isolates examined were capable of growth at −1 °C. Mrakia spp., while capable of growth at −1 °C, did not demonstrate any antifreeze activity and exhibited only limited secretion of extracellular polysaccharides. Species of the genus Mrakia possessed high amounts of unsaturated fatty acids, suggesting that members of this genus have adapted to cold environments by increasing their membrane fluidity. |
| doi_str_mv | 10.1111/1574-6968.12217 |
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A total of 71 isolates were collected from lake sediment and soil surrounding lakes in the Skarvsnes area, Antarctica. Based on ITS region sequence similarity, these isolates were classified to 10 genera. Twenty-three isolates were categorized as ascomycetous fungi from five genera (Embellisia, Phoma, Geomyces, Tetracladium or Thelebolus) and 48 isolates were categorized as basidiomycetous fungi in five genera (Mrakia, Cryptococcus, Dioszegia, Rhodotorula or Leucosporidium). Thirty-five percent of culturable fungi were of the genus Mrakia. Eighteen isolates from eight genera were selected and tested for both antifreeze activity and capacity for growth under temperatures ranging from −1 to 25 °C. Rhodotorula sp. NHT-2 possessed a high degree of sequence homology with R. gracialis, while Leucosporidium sp. BSS-1 possessed a high degree of sequence homology with Leu. antarcticum (Glaciozyma antarctica), and these two isolates demonstrated antifreeze activity. All isolates examined were capable of growth at −1 °C. Mrakia spp., while capable of growth at −1 °C, did not demonstrate any antifreeze activity and exhibited only limited secretion of extracellular polysaccharides. Species of the genus Mrakia possessed high amounts of unsaturated fatty acids, suggesting that members of this genus have adapted to cold environments by increasing their membrane fluidity.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/1574-6968.12217</identifier><identifier>PMID: 23862768</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adaptation, Physiological - genetics ; Antarctic Regions ; Ascomycota - classification ; Ascomycota - genetics ; Ascomycota - isolation & purification ; Ascomycota - physiology ; Basidiomycota ; Basidiomycota - classification ; Basidiomycota - genetics ; Basidiomycota - isolation & purification ; Basidiomycota - physiology ; biodiversity ; Biological and medical sciences ; cold adaptation ; Cold Temperature ; Cold-Shock Response - genetics ; Cold-Shock Response - physiology ; cryophilic fungi ; Cryptococcus ; DNA, Fungal - analysis ; DNA, Fungal - genetics ; Embellisia ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Geologic Sediments - microbiology ; Lake sediments ; Lakes ; Leucosporidium ; Microbiology ; Miscellaneous ; Mycology ; Phoma ; Rhodotorula ; Saccharides ; Skarvsnes ice‐free area ; Soil Microbiology ; Thelebolus</subject><ispartof>FEMS microbiology letters, 2013-09, Vol.346 (2), p.121-130</ispartof><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved 2013</rights><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2014 INIST-CNRS</rights><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5767-48f41809d71a5fe14ee527fcab798f4fc0c846ff324ba8b4252d05d75b7bae723</citedby><cites>FETCH-LOGICAL-c5767-48f41809d71a5fe14ee527fcab798f4fc0c846ff324ba8b4252d05d75b7bae723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1574-6968.12217$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1574-6968.12217$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27674043$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23862768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsuji, Masaharu</creatorcontrib><creatorcontrib>Fujiu, Seiichi</creatorcontrib><creatorcontrib>Xiao, Nan</creatorcontrib><creatorcontrib>Hanada, Yuichi</creatorcontrib><creatorcontrib>Kudoh, Sakae</creatorcontrib><creatorcontrib>Kondo, Hidemasa</creatorcontrib><creatorcontrib>Tsuda, Sakae</creatorcontrib><creatorcontrib>Hoshino, Tamotsu</creatorcontrib><title>Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
A total of 71 isolates were collected from lake sediment and soil surrounding lakes in the Skarvsnes area, Antarctica. Based on ITS region sequence similarity, these isolates were classified to 10 genera. Twenty-three isolates were categorized as ascomycetous fungi from five genera (Embellisia, Phoma, Geomyces, Tetracladium or Thelebolus) and 48 isolates were categorized as basidiomycetous fungi in five genera (Mrakia, Cryptococcus, Dioszegia, Rhodotorula or Leucosporidium). Thirty-five percent of culturable fungi were of the genus Mrakia. Eighteen isolates from eight genera were selected and tested for both antifreeze activity and capacity for growth under temperatures ranging from −1 to 25 °C. Rhodotorula sp. NHT-2 possessed a high degree of sequence homology with R. gracialis, while Leucosporidium sp. BSS-1 possessed a high degree of sequence homology with Leu. antarcticum (Glaciozyma antarctica), and these two isolates demonstrated antifreeze activity. All isolates examined were capable of growth at −1 °C. Mrakia spp., while capable of growth at −1 °C, did not demonstrate any antifreeze activity and exhibited only limited secretion of extracellular polysaccharides. Species of the genus Mrakia possessed high amounts of unsaturated fatty acids, suggesting that members of this genus have adapted to cold environments by increasing their membrane fluidity.</description><subject>Adaptation, Physiological - genetics</subject><subject>Antarctic Regions</subject><subject>Ascomycota - classification</subject><subject>Ascomycota - genetics</subject><subject>Ascomycota - isolation & purification</subject><subject>Ascomycota - physiology</subject><subject>Basidiomycota</subject><subject>Basidiomycota - classification</subject><subject>Basidiomycota - genetics</subject><subject>Basidiomycota - isolation & purification</subject><subject>Basidiomycota - physiology</subject><subject>biodiversity</subject><subject>Biological and medical sciences</subject><subject>cold adaptation</subject><subject>Cold Temperature</subject><subject>Cold-Shock Response - genetics</subject><subject>Cold-Shock Response - physiology</subject><subject>cryophilic fungi</subject><subject>Cryptococcus</subject><subject>DNA, Fungal - analysis</subject><subject>DNA, Fungal - genetics</subject><subject>Embellisia</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geologic Sediments - microbiology</subject><subject>Lake sediments</subject><subject>Lakes</subject><subject>Leucosporidium</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Phoma</subject><subject>Rhodotorula</subject><subject>Saccharides</subject><subject>Skarvsnes ice‐free area</subject><subject>Soil Microbiology</subject><subject>Thelebolus</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdFrFDEQxoMo9jx99k0CIhRx2ySbbHYfy9Fq4cQH9TnMZic27W5yJrtK_3v3etdWilDzMpD8vsk38xHymrMjPp9jrrQsqqaqj7gQXD8hi7ubp2TBSl0XnDX6gLzI-ZIxJgWrnpMDUdaV0FW9IBer2HcUOtiMMPoYaHTUTeGHp7EdwQfsqEtxoDn6nkLoaA9XSDN2fsAwUh_oeIH06xWkXzlgpt5i4RIihYTwgZ6EEZIdvYWX5JmDPuOrfV2S72en31afivWXj-erk3Vhla50IWsnec2aTnNQDrlEVEI7C61u5idnma1l5VwpZAt1K4USHVOdVq1uAbUol-Rw13eT4s8J82gGny32PQSMUzZclo0QtRL_hZZCczGXJXn7AL2MUwrzIIarknHNy4bP1PGOsinmnNCZTfIDpGvDmdnmZbbpmG065iavWfFm33dqB-zu-NuAZuDdHoBsoXcJgvX5npt3JtmNQbXjfvserx_715x9Xt8aeL_TxWnzT1Xxl9s_DRS22w</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Tsuji, Masaharu</creator><creator>Fujiu, Seiichi</creator><creator>Xiao, Nan</creator><creator>Hanada, Yuichi</creator><creator>Kudoh, Sakae</creator><creator>Kondo, Hidemasa</creator><creator>Tsuda, Sakae</creator><creator>Hoshino, Tamotsu</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7ST</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>SOI</scope></search><sort><creationdate>201309</creationdate><title>Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica</title><author>Tsuji, Masaharu ; Fujiu, Seiichi ; Xiao, Nan ; Hanada, Yuichi ; Kudoh, Sakae ; Kondo, Hidemasa ; Tsuda, Sakae ; Hoshino, Tamotsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5767-48f41809d71a5fe14ee527fcab798f4fc0c846ff324ba8b4252d05d75b7bae723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adaptation, Physiological - genetics</topic><topic>Antarctic Regions</topic><topic>Ascomycota - classification</topic><topic>Ascomycota - genetics</topic><topic>Ascomycota - isolation & purification</topic><topic>Ascomycota - physiology</topic><topic>Basidiomycota</topic><topic>Basidiomycota - classification</topic><topic>Basidiomycota - genetics</topic><topic>Basidiomycota - isolation & purification</topic><topic>Basidiomycota - physiology</topic><topic>biodiversity</topic><topic>Biological and medical sciences</topic><topic>cold adaptation</topic><topic>Cold Temperature</topic><topic>Cold-Shock Response - genetics</topic><topic>Cold-Shock Response - physiology</topic><topic>cryophilic fungi</topic><topic>Cryptococcus</topic><topic>DNA, Fungal - analysis</topic><topic>DNA, Fungal - genetics</topic><topic>Embellisia</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geologic Sediments - microbiology</topic><topic>Lake sediments</topic><topic>Lakes</topic><topic>Leucosporidium</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Phoma</topic><topic>Rhodotorula</topic><topic>Saccharides</topic><topic>Skarvsnes ice‐free area</topic><topic>Soil Microbiology</topic><topic>Thelebolus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsuji, Masaharu</creatorcontrib><creatorcontrib>Fujiu, Seiichi</creatorcontrib><creatorcontrib>Xiao, Nan</creatorcontrib><creatorcontrib>Hanada, Yuichi</creatorcontrib><creatorcontrib>Kudoh, Sakae</creatorcontrib><creatorcontrib>Kondo, Hidemasa</creatorcontrib><creatorcontrib>Tsuda, Sakae</creatorcontrib><creatorcontrib>Hoshino, Tamotsu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsuji, Masaharu</au><au>Fujiu, Seiichi</au><au>Xiao, Nan</au><au>Hanada, Yuichi</au><au>Kudoh, Sakae</au><au>Kondo, Hidemasa</au><au>Tsuda, Sakae</au><au>Hoshino, Tamotsu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2013-09</date><risdate>2013</risdate><volume>346</volume><issue>2</issue><spage>121</spage><epage>130</epage><pages>121-130</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
A total of 71 isolates were collected from lake sediment and soil surrounding lakes in the Skarvsnes area, Antarctica. Based on ITS region sequence similarity, these isolates were classified to 10 genera. Twenty-three isolates were categorized as ascomycetous fungi from five genera (Embellisia, Phoma, Geomyces, Tetracladium or Thelebolus) and 48 isolates were categorized as basidiomycetous fungi in five genera (Mrakia, Cryptococcus, Dioszegia, Rhodotorula or Leucosporidium). Thirty-five percent of culturable fungi were of the genus Mrakia. Eighteen isolates from eight genera were selected and tested for both antifreeze activity and capacity for growth under temperatures ranging from −1 to 25 °C. Rhodotorula sp. NHT-2 possessed a high degree of sequence homology with R. gracialis, while Leucosporidium sp. BSS-1 possessed a high degree of sequence homology with Leu. antarcticum (Glaciozyma antarctica), and these two isolates demonstrated antifreeze activity. All isolates examined were capable of growth at −1 °C. Mrakia spp., while capable of growth at −1 °C, did not demonstrate any antifreeze activity and exhibited only limited secretion of extracellular polysaccharides. Species of the genus Mrakia possessed high amounts of unsaturated fatty acids, suggesting that members of this genus have adapted to cold environments by increasing their membrane fluidity.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>23862768</pmid><doi>10.1111/1574-6968.12217</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptation, Physiological - genetics Antarctic Regions Ascomycota - classification Ascomycota - genetics Ascomycota - isolation & purification Ascomycota - physiology Basidiomycota Basidiomycota - classification Basidiomycota - genetics Basidiomycota - isolation & purification Basidiomycota - physiology biodiversity Biological and medical sciences cold adaptation Cold Temperature Cold-Shock Response - genetics Cold-Shock Response - physiology cryophilic fungi Cryptococcus DNA, Fungal - analysis DNA, Fungal - genetics Embellisia Freshwater Fundamental and applied biological sciences. Psychology Geologic Sediments - microbiology Lake sediments Lakes Leucosporidium Microbiology Miscellaneous Mycology Phoma Rhodotorula Saccharides Skarvsnes ice‐free area Soil Microbiology Thelebolus |
| title | Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica |
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