O-Antigen Protects Gram-Negative Bacteria from Histone Killing. e71097
Beyond their traditional role of wrapping DNA, histones display antibacterial activity to Gram-negative and -positive bacteria. To identify bacterial components that allow survival to a histone challenge, we selected resistant bacteria from homologous Escherichia coli libraries that harbor plasmids...
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| Veröffentlicht in: | PloS one 2013-08, Vol.8 (8) |
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| creator | Chaput, Catherine Spindler, Eileen Gill, Ryan T Zychlinsky, Arturo |
| description | Beyond their traditional role of wrapping DNA, histones display antibacterial activity to Gram-negative and -positive bacteria. To identify bacterial components that allow survival to a histone challenge, we selected resistant bacteria from homologous Escherichia coli libraries that harbor plasmids carrying pieces of the chromosome in different sizes. We identified genes required for exopolysaccharide production and for the synthesis of the polysaccharide domain of the lipopolysaccharide, called O-antigen. Indeed, O-antigen and exopolysaccharide conferred further resistance to histones. Notably, O-antigen also conferred resistance to histones in the pathogens Shigella flexneri and Klebsiella pneumoniae. |
| doi_str_mv | 10.1371/journal.pone.0071097 |
| format | Article |
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| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Escherichia coli Klebsiella pneumoniae Shigella flexneri |
| title | O-Antigen Protects Gram-Negative Bacteria from Histone Killing. e71097 |
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