Localization of ribulose 1,5-bisphosphate carboxylase/oxygenase in the N sub(2)-fixing cyanobacterium Anabaena cylindrica

Localization of ribulose 1,5-bisphosphate carboxylase/oxygenase in the N sub(2)-fixing cyanobacterium Anabaena cylindrica

Monospecific antiserum against ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) of Microcystis PCC7820 cross-reacted with cell-free extracts of the heterocystous cyanobacterium A. cylindrica . This antiserum was used in conjuction with colloidal gold to localize RuBisCO protein in filaments...

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Veröffentlicht in:FEMS microbiology letters 1985-01, Vol.28 (1), p.65-68
Hauptverfasser: Cossar, J D, Rowell, P, Darling, A J, Murray, S, Codd, G A, Stewart, WDP
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Anabaena cylindrica
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container_issue 1
container_start_page 65
container_title FEMS microbiology letters
container_volume 28
creator Cossar, J D
Rowell, P
Darling, A J
Murray, S
Codd, G A
Stewart, WDP
description Monospecific antiserum against ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) of Microcystis PCC7820 cross-reacted with cell-free extracts of the heterocystous cyanobacterium A. cylindrica . This antiserum was used in conjuction with colloidal gold to localize RuBisCO protein in filaments of A. cylindrica . Labelling occurred in the vegetative cells where it was associated particularly with carboxysomes in stationary phase cultures and with both carboxysomes and chromatoplasm in continuous cultures, with the proportion of RuBisCO in the chromatoplasm increasing with increasing growth rate. The protein was undetectable in mature heterocysts.
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source Oxford University Press Journals Digital Archive legacy; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection
subjects Anabaena cylindrica
title Localization of ribulose 1,5-bisphosphate carboxylase/oxygenase in the N sub(2)-fixing cyanobacterium Anabaena cylindrica
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