Application of cetylpyridinium chloride and sodium chloride decontamination method for recovery of Mycobacterium tuberculosis from clinically suspected cases of pulmonary tuberculosis
Abstract The study was designed to compare the efficacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) fr...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2013-10, Vol.77 (2), p.150-157 |
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| creator | Shinu, Pottathil Singh, Varsha Nair, Anroop Mehrishi, Priya Mehta, Sonia Joshi, Ekta |
| description | Abstract The study was designed to compare the efficacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) from clinically suspected cases of pulmonary tuberculosis. To evaluate CPC-NaCl and NALC-NaOH decontamination methods, sputum specimens (n = 796) were studied (culturing on Löwenstein-Jensen medium), and the performances were compared. The CPC-NaCl decontamination method demonstrated a sensitivity, specificity, negative predictive value, and positive predictive value of 97.99%, 87.53%, 70.19%, and 99.32%, respectively, when compared to NALC-NaOH decontamination method. In summary, CPC-NaCl decontamination method effectively detected significantly higher number of MTB cases (n = 208) than NALC-NaOH decontamination method (n = 149) particularly in sputum with scanty bacilli and smear-negative cases, indicating the potential of CPC-NaCl decontamination method to preserve paucibacillary cases more efficient than NALC-NaOH decontamination method. |
| doi_str_mv | 10.1016/j.diagmicrobio.2013.06.021 |
| format | Article |
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To evaluate CPC-NaCl and NALC-NaOH decontamination methods, sputum specimens (n = 796) were studied (culturing on Löwenstein-Jensen medium), and the performances were compared. The CPC-NaCl decontamination method demonstrated a sensitivity, specificity, negative predictive value, and positive predictive value of 97.99%, 87.53%, 70.19%, and 99.32%, respectively, when compared to NALC-NaOH decontamination method. In summary, CPC-NaCl decontamination method effectively detected significantly higher number of MTB cases (n = 208) than NALC-NaOH decontamination method (n = 149) particularly in sputum with scanty bacilli and smear-negative cases, indicating the potential of CPC-NaCl decontamination method to preserve paucibacillary cases more efficient than NALC-NaOH decontamination method.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2013.06.021</identifier><identifier>PMID: 23928270</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Bacteriological Techniques - methods ; Cetylpyridinium - chemistry ; Cetylpyridinium chloride ; Cystine - analogs & derivatives ; Cystine - chemistry ; Decontamination ; Direct microscopy ; Female ; Humans ; Infectious Disease ; Internal Medicine ; Male ; Microscopy ; Mycobacterium tuberculosis - isolation & purification ; N-acetyl L-Cystine ; Pulmonary tuberculosis ; Reproducibility of Results ; Sensitivity and Specificity ; Sodium Chloride - chemistry ; Sputum - microbiology ; Tuberculosis, Pulmonary - diagnosis ; Tuberculosis, Pulmonary - microbiology</subject><ispartof>Diagnostic microbiology and infectious disease, 2013-10, Vol.77 (2), p.150-157</ispartof><rights>Elsevier Inc.</rights><rights>2013 Elsevier Inc.</rights><rights>2013.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-f37cb8a9b621ff12e2ff804b2992aa4cebf01f33e5633c3a75ca98132b11c39e3</citedby><cites>FETCH-LOGICAL-c435t-f37cb8a9b621ff12e2ff804b2992aa4cebf01f33e5633c3a75ca98132b11c39e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889313003763$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23928270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shinu, Pottathil</creatorcontrib><creatorcontrib>Singh, Varsha</creatorcontrib><creatorcontrib>Nair, Anroop</creatorcontrib><creatorcontrib>Mehrishi, Priya</creatorcontrib><creatorcontrib>Mehta, Sonia</creatorcontrib><creatorcontrib>Joshi, Ekta</creatorcontrib><title>Application of cetylpyridinium chloride and sodium chloride decontamination method for recovery of Mycobacterium tuberculosis from clinically suspected cases of pulmonary tuberculosis</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract The study was designed to compare the efficacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) from clinically suspected cases of pulmonary tuberculosis. To evaluate CPC-NaCl and NALC-NaOH decontamination methods, sputum specimens (n = 796) were studied (culturing on Löwenstein-Jensen medium), and the performances were compared. The CPC-NaCl decontamination method demonstrated a sensitivity, specificity, negative predictive value, and positive predictive value of 97.99%, 87.53%, 70.19%, and 99.32%, respectively, when compared to NALC-NaOH decontamination method. In summary, CPC-NaCl decontamination method effectively detected significantly higher number of MTB cases (n = 208) than NALC-NaOH decontamination method (n = 149) particularly in sputum with scanty bacilli and smear-negative cases, indicating the potential of CPC-NaCl decontamination method to preserve paucibacillary cases more efficient than NALC-NaOH decontamination method.</description><subject>Adult</subject><subject>Bacteriological Techniques - methods</subject><subject>Cetylpyridinium - chemistry</subject><subject>Cetylpyridinium chloride</subject><subject>Cystine - analogs & derivatives</subject><subject>Cystine - chemistry</subject><subject>Decontamination</subject><subject>Direct microscopy</subject><subject>Female</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Microscopy</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>N-acetyl L-Cystine</subject><subject>Pulmonary tuberculosis</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Sodium Chloride - chemistry</subject><subject>Sputum - microbiology</subject><subject>Tuberculosis, Pulmonary - diagnosis</subject><subject>Tuberculosis, Pulmonary - microbiology</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUs2O1SAYJUbj3Bl9BUNcuWn9gN62uDCZzDhqMsaFuiaUfjhcaanQTtIn8_WkuTNmdOWKAOcHzvkIecmgZMDq14eyd_r74EwMnQslByZKqEvg7BHZsbaRBUADj8kOGsGLtpXihJymdABgXFbwlJxwIXnLG9iRX-fT5J3RswsjDZYanFc_rdH1bnTLQM2ND3mDVI89TaH_66xHE8ZZD2488gecb0JPbYg05qtbjOum-Wk1odNmxrix56XDaBYfkkvUxpD1fPYy2vuVpiVNmJE9NTph2tjT4ocw6iz1kPmMPLHaJ3x-t56Rb1fvvl58KK4_v_94cX5dmErs58KKxnStll3NmbWMI7e2harjUnKtK4OdBWaFwH0thBG62RstWyZ4x5gREsUZeXXUnWL4uWCa1eCSQe_1iGFJilWi2bdcNpChb47Q3EtKEa2aohvywxUDtRWnDuphcWorTkGtcnGZ_OLOZ-kG7P9Q75vKgMsjAPNvbx1GlYzD0WDvctaz6oP7P5-3_8jcp_8DV0yHsMQx56mYSlyB-rKN0DZBTACIJof0G-_qzNM</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Shinu, Pottathil</creator><creator>Singh, Varsha</creator><creator>Nair, Anroop</creator><creator>Mehrishi, Priya</creator><creator>Mehta, Sonia</creator><creator>Joshi, Ekta</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131001</creationdate><title>Application of cetylpyridinium chloride and sodium chloride decontamination method for recovery of Mycobacterium tuberculosis from clinically suspected cases of pulmonary tuberculosis</title><author>Shinu, Pottathil ; Singh, Varsha ; Nair, Anroop ; Mehrishi, Priya ; Mehta, Sonia ; Joshi, Ekta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-f37cb8a9b621ff12e2ff804b2992aa4cebf01f33e5633c3a75ca98132b11c39e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Bacteriological Techniques - methods</topic><topic>Cetylpyridinium - chemistry</topic><topic>Cetylpyridinium chloride</topic><topic>Cystine - analogs & derivatives</topic><topic>Cystine - chemistry</topic><topic>Decontamination</topic><topic>Direct microscopy</topic><topic>Female</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Microscopy</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>N-acetyl L-Cystine</topic><topic>Pulmonary tuberculosis</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Sodium Chloride - chemistry</topic><topic>Sputum - microbiology</topic><topic>Tuberculosis, Pulmonary - diagnosis</topic><topic>Tuberculosis, Pulmonary - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shinu, Pottathil</creatorcontrib><creatorcontrib>Singh, Varsha</creatorcontrib><creatorcontrib>Nair, Anroop</creatorcontrib><creatorcontrib>Mehrishi, Priya</creatorcontrib><creatorcontrib>Mehta, Sonia</creatorcontrib><creatorcontrib>Joshi, Ekta</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shinu, Pottathil</au><au>Singh, Varsha</au><au>Nair, Anroop</au><au>Mehrishi, Priya</au><au>Mehta, Sonia</au><au>Joshi, Ekta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of cetylpyridinium chloride and sodium chloride decontamination method for recovery of Mycobacterium tuberculosis from clinically suspected cases of pulmonary tuberculosis</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>77</volume><issue>2</issue><spage>150</spage><epage>157</epage><pages>150-157</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>Abstract The study was designed to compare the efficacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) from clinically suspected cases of pulmonary tuberculosis. To evaluate CPC-NaCl and NALC-NaOH decontamination methods, sputum specimens (n = 796) were studied (culturing on Löwenstein-Jensen medium), and the performances were compared. The CPC-NaCl decontamination method demonstrated a sensitivity, specificity, negative predictive value, and positive predictive value of 97.99%, 87.53%, 70.19%, and 99.32%, respectively, when compared to NALC-NaOH decontamination method. In summary, CPC-NaCl decontamination method effectively detected significantly higher number of MTB cases (n = 208) than NALC-NaOH decontamination method (n = 149) particularly in sputum with scanty bacilli and smear-negative cases, indicating the potential of CPC-NaCl decontamination method to preserve paucibacillary cases more efficient than NALC-NaOH decontamination method.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23928270</pmid><doi>10.1016/j.diagmicrobio.2013.06.021</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Bacteriological Techniques - methods Cetylpyridinium - chemistry Cetylpyridinium chloride Cystine - analogs & derivatives Cystine - chemistry Decontamination Direct microscopy Female Humans Infectious Disease Internal Medicine Male Microscopy Mycobacterium tuberculosis - isolation & purification N-acetyl L-Cystine Pulmonary tuberculosis Reproducibility of Results Sensitivity and Specificity Sodium Chloride - chemistry Sputum - microbiology Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - microbiology |
| title | Application of cetylpyridinium chloride and sodium chloride decontamination method for recovery of Mycobacterium tuberculosis from clinically suspected cases of pulmonary tuberculosis |
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