Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers

Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers

The genetic diversity among 28 pea (Pisum sativum L.) genotypes was analyzed using 32 simple sequence repeat markers. A total of 44 polymorphic bands, with an average of 2.1 bands per primer, were obtained. The polymorphism information content ranged from 0.657 to 0.309 with an average of 0.493. The...

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Veröffentlicht in:Genetics and molecular research 2013-03, Vol.12 (3), p.3540-3550
Hauptverfasser: Kumari, P, Basal, N, Singh, A K, Rai, V P, Srivastava, C P, Singh, P K
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Genetic Markers
Genetic Variation
Genotype
Microsatellite Repeats - genetics
Phylogeny
Pisum sativum - genetics
Principal Component Analysis
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creator Kumari, P
Basal, N
Singh, A K
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Srivastava, C P
Singh, P K
description The genetic diversity among 28 pea (Pisum sativum L.) genotypes was analyzed using 32 simple sequence repeat markers. A total of 44 polymorphic bands, with an average of 2.1 bands per primer, were obtained. The polymorphism information content ranged from 0.657 to 0.309 with an average of 0.493. The variation in genetic diversity among these cultivars ranged from 0.11 to 0.73. Cluster analysis based on Jaccard's similarity coefficient using the unweighted pair-group method with arithmetic mean (UPGMA) revealed 2 distinct clusters, I and II, comprising 6 and 22 genotypes, respectively. Cluster II was further differentiated into 2 subclusters, IIA and IIB, with 12 and 10 genotypes, respectively. Principal component (PC) analysis revealed results similar to those of UPGMA. The first, second, and third PCs contributed 21.6, 16.1, and 14.0% of the variation, respectively; cumulative variation of the first 3 PCs was 51.7%.
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subjects Genetic Markers
Genetic Variation
Genotype
Microsatellite Repeats - genetics
Phylogeny
Pisum sativum - genetics
Principal Component Analysis
title Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers
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