In vitro Expression of Chloroplast Genes in Lysates of Higher Plant Chloroplasts
A DNA-dependent in vitro-coupled transcription--translation system has been prepared from lysates of isolated chloroplasts. These lysates are comparable to those of Escherichia coli in transcriptional and translational fidelity and efficiency in response to a given template DNA. When Nicotiana tabac...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-06, Vol.82 (12), p.3983-3987 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3987 |
|---|---|
| container_issue | 12 |
| container_start_page | 3983 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 82 |
| creator | Bard, Julie Bourque, Don P. Hildebrand, Mark Zaitlin, David |
| description | A DNA-dependent in vitro-coupled transcription--translation system has been prepared from lysates of isolated chloroplasts. These lysates are comparable to those of Escherichia coli in transcriptional and translational fidelity and efficiency in response to a given template DNA. When Nicotiana tabacum chloroplast DNA is used as template with chloroplast lysates (N. tabacum or spinach) or E. coli lysates, NaDodSO4 gel analysis reveals similar polypeptide patterns that are distinct from the patterns obtained with E. coli DNA. Genes in recombinant plasmids containing chloroplast DNA are also expressed in these in vitro systems. DNA· RNA hybridization experiments show that transcripts are synthesized from most of the chloroplast genome. Newly synthesized large subunit of ribulosebisphosphate carboxylase/oxygenate and a transcript of the large subunit gene (rbcL) are observed in chloroplast lysates using as template chloroplast DNA or cloned fragments of tobacco chloroplast DNA that contain the large subunit gene. Results suggest that differential expression of chloroplast genes occurs in vitro. By using cloned chloroplast DNA templates in this homologous system, it is possible to identify and map structural genes for chloroplast proteins. |
| doi_str_mv | 10.1073/pnas.82.12.3983 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_14370671</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>26014</jstor_id><sourcerecordid>26014</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4363-6caf245f8eb6ed62d65cad8a777066e4bc2503a3c9fd65ba5eb63667296b01d43</originalsourceid><addsrcrecordid>eNp9kUtr3DAUhUVpSCdp14VCixehWXmih_VadFGGvGCgWbRrIctyRkFjuZImJP8-MuMOyaYrCc53H-ceAD4juESQk4tx0Gkp8BLhJZGCvAMLBCWqWSPhe7CAEPNaNLj5AE5SeoAQSirgMTgmggpB6QLc3Q7Vo8sxVJdPY7QpuTBUoa9WGx9iGL1Oubq2g02VG6r1c9K5fIt-4-43NlZ3Xg_5NZw-gqNe-2Q_ze8p-HN1-Xt1U69_Xd-ufq5r0xBGamZ0jxvaC9sy2zHcMWp0JzTnHDJmm9ZgCokmRvZFajUtHGGMY8laiLqGnIIf-77jrt3aztghR-3VGN1Wx2cVtFNvlcFt1H14VERyiUSp_z7Xx_B3Z1NWW5eM9cWQDbukUEPKJhwV8GIPmhhSirY_zEBQTRmoKQMlsEJYTRmUiq-vVzvw89GLfjbrOhnt-6gH49IBk7jhVE4Ov83Y1P-f-mbO-X8B1e-8z_YpF_LLnnxIOcQDihksNl8A6YSx5A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14370671</pqid></control><display><type>article</type><title>In vitro Expression of Chloroplast Genes in Lysates of Higher Plant Chloroplasts</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Bard, Julie ; Bourque, Don P. ; Hildebrand, Mark ; Zaitlin, David</creator><creatorcontrib>Bard, Julie ; Bourque, Don P. ; Hildebrand, Mark ; Zaitlin, David</creatorcontrib><description>A DNA-dependent in vitro-coupled transcription--translation system has been prepared from lysates of isolated chloroplasts. These lysates are comparable to those of Escherichia coli in transcriptional and translational fidelity and efficiency in response to a given template DNA. When Nicotiana tabacum chloroplast DNA is used as template with chloroplast lysates (N. tabacum or spinach) or E. coli lysates, NaDodSO4 gel analysis reveals similar polypeptide patterns that are distinct from the patterns obtained with E. coli DNA. Genes in recombinant plasmids containing chloroplast DNA are also expressed in these in vitro systems. DNA· RNA hybridization experiments show that transcripts are synthesized from most of the chloroplast genome. Newly synthesized large subunit of ribulosebisphosphate carboxylase/oxygenate and a transcript of the large subunit gene (rbcL) are observed in chloroplast lysates using as template chloroplast DNA or cloned fragments of tobacco chloroplast DNA that contain the large subunit gene. Results suggest that differential expression of chloroplast genes occurs in vitro. By using cloned chloroplast DNA templates in this homologous system, it is possible to identify and map structural genes for chloroplast proteins.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.12.3983</identifier><identifier>PMID: 3858855</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Bards ; Biological and medical sciences ; Cell-Free System ; Chloroplast DNA ; Chloroplasts ; Chloroplasts - physiology ; DNA ; DNA - genetics ; Fundamental and applied biological sciences. Psychology ; Gels ; Gene expression ; Gene Expression Regulation ; Genes ; Molecular and cellular biology ; Molecular genetics ; Nicotiana - genetics ; Nicotiana tabacum ; Plant cells ; Plants, Toxic ; Plasmids ; Protein Biosynthesis ; Ribulose-Bisphosphate Carboxylase - genetics ; RNA ; Spinach ; Transcription, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-06, Vol.82 (12), p.3983-3987</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4363-6caf245f8eb6ed62d65cad8a777066e4bc2503a3c9fd65ba5eb63667296b01d43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26014$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26014$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9247594$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3858855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bard, Julie</creatorcontrib><creatorcontrib>Bourque, Don P.</creatorcontrib><creatorcontrib>Hildebrand, Mark</creatorcontrib><creatorcontrib>Zaitlin, David</creatorcontrib><title>In vitro Expression of Chloroplast Genes in Lysates of Higher Plant Chloroplasts</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A DNA-dependent in vitro-coupled transcription--translation system has been prepared from lysates of isolated chloroplasts. These lysates are comparable to those of Escherichia coli in transcriptional and translational fidelity and efficiency in response to a given template DNA. When Nicotiana tabacum chloroplast DNA is used as template with chloroplast lysates (N. tabacum or spinach) or E. coli lysates, NaDodSO4 gel analysis reveals similar polypeptide patterns that are distinct from the patterns obtained with E. coli DNA. Genes in recombinant plasmids containing chloroplast DNA are also expressed in these in vitro systems. DNA· RNA hybridization experiments show that transcripts are synthesized from most of the chloroplast genome. Newly synthesized large subunit of ribulosebisphosphate carboxylase/oxygenate and a transcript of the large subunit gene (rbcL) are observed in chloroplast lysates using as template chloroplast DNA or cloned fragments of tobacco chloroplast DNA that contain the large subunit gene. Results suggest that differential expression of chloroplast genes occurs in vitro. By using cloned chloroplast DNA templates in this homologous system, it is possible to identify and map structural genes for chloroplast proteins.</description><subject>Bards</subject><subject>Biological and medical sciences</subject><subject>Cell-Free System</subject><subject>Chloroplast DNA</subject><subject>Chloroplasts</subject><subject>Chloroplasts - physiology</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana tabacum</subject><subject>Plant cells</subject><subject>Plants, Toxic</subject><subject>Plasmids</subject><subject>Protein Biosynthesis</subject><subject>Ribulose-Bisphosphate Carboxylase - genetics</subject><subject>RNA</subject><subject>Spinach</subject><subject>Transcription, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtr3DAUhUVpSCdp14VCixehWXmih_VadFGGvGCgWbRrIctyRkFjuZImJP8-MuMOyaYrCc53H-ceAD4juESQk4tx0Gkp8BLhJZGCvAMLBCWqWSPhe7CAEPNaNLj5AE5SeoAQSirgMTgmggpB6QLc3Q7Vo8sxVJdPY7QpuTBUoa9WGx9iGL1Oubq2g02VG6r1c9K5fIt-4-43NlZ3Xg_5NZw-gqNe-2Q_ze8p-HN1-Xt1U69_Xd-ufq5r0xBGamZ0jxvaC9sy2zHcMWp0JzTnHDJmm9ZgCokmRvZFajUtHGGMY8laiLqGnIIf-77jrt3aztghR-3VGN1Wx2cVtFNvlcFt1H14VERyiUSp_z7Xx_B3Z1NWW5eM9cWQDbukUEPKJhwV8GIPmhhSirY_zEBQTRmoKQMlsEJYTRmUiq-vVzvw89GLfjbrOhnt-6gH49IBk7jhVE4Ov83Y1P-f-mbO-X8B1e-8z_YpF_LLnnxIOcQDihksNl8A6YSx5A</recordid><startdate>19850601</startdate><enddate>19850601</enddate><creator>Bard, Julie</creator><creator>Bourque, Don P.</creator><creator>Hildebrand, Mark</creator><creator>Zaitlin, David</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19850601</creationdate><title>In vitro Expression of Chloroplast Genes in Lysates of Higher Plant Chloroplasts</title><author>Bard, Julie ; Bourque, Don P. ; Hildebrand, Mark ; Zaitlin, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4363-6caf245f8eb6ed62d65cad8a777066e4bc2503a3c9fd65ba5eb63667296b01d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Bards</topic><topic>Biological and medical sciences</topic><topic>Cell-Free System</topic><topic>Chloroplast DNA</topic><topic>Chloroplasts</topic><topic>Chloroplasts - physiology</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana tabacum</topic><topic>Plant cells</topic><topic>Plants, Toxic</topic><topic>Plasmids</topic><topic>Protein Biosynthesis</topic><topic>Ribulose-Bisphosphate Carboxylase - genetics</topic><topic>RNA</topic><topic>Spinach</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bard, Julie</creatorcontrib><creatorcontrib>Bourque, Don P.</creatorcontrib><creatorcontrib>Hildebrand, Mark</creatorcontrib><creatorcontrib>Zaitlin, David</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bard, Julie</au><au>Bourque, Don P.</au><au>Hildebrand, Mark</au><au>Zaitlin, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro Expression of Chloroplast Genes in Lysates of Higher Plant Chloroplasts</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-06-01</date><risdate>1985</risdate><volume>82</volume><issue>12</issue><spage>3983</spage><epage>3987</epage><pages>3983-3987</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A DNA-dependent in vitro-coupled transcription--translation system has been prepared from lysates of isolated chloroplasts. These lysates are comparable to those of Escherichia coli in transcriptional and translational fidelity and efficiency in response to a given template DNA. When Nicotiana tabacum chloroplast DNA is used as template with chloroplast lysates (N. tabacum or spinach) or E. coli lysates, NaDodSO4 gel analysis reveals similar polypeptide patterns that are distinct from the patterns obtained with E. coli DNA. Genes in recombinant plasmids containing chloroplast DNA are also expressed in these in vitro systems. DNA· RNA hybridization experiments show that transcripts are synthesized from most of the chloroplast genome. Newly synthesized large subunit of ribulosebisphosphate carboxylase/oxygenate and a transcript of the large subunit gene (rbcL) are observed in chloroplast lysates using as template chloroplast DNA or cloned fragments of tobacco chloroplast DNA that contain the large subunit gene. Results suggest that differential expression of chloroplast genes occurs in vitro. By using cloned chloroplast DNA templates in this homologous system, it is possible to identify and map structural genes for chloroplast proteins.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3858855</pmid><doi>10.1073/pnas.82.12.3983</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-06, Vol.82 (12), p.3983-3987 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14370671 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Bards Biological and medical sciences Cell-Free System Chloroplast DNA Chloroplasts Chloroplasts - physiology DNA DNA - genetics Fundamental and applied biological sciences. Psychology Gels Gene expression Gene Expression Regulation Genes Molecular and cellular biology Molecular genetics Nicotiana - genetics Nicotiana tabacum Plant cells Plants, Toxic Plasmids Protein Biosynthesis Ribulose-Bisphosphate Carboxylase - genetics RNA Spinach Transcription, Genetic |
| title | In vitro Expression of Chloroplast Genes in Lysates of Higher Plant Chloroplasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T04%3A57%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20Expression%20of%20Chloroplast%20Genes%20in%20Lysates%20of%20Higher%20Plant%20Chloroplasts&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Bard,%20Julie&rft.date=1985-06-01&rft.volume=82&rft.issue=12&rft.spage=3983&rft.epage=3987&rft.pages=3983-3987&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.82.12.3983&rft_dat=%3Cjstor_proqu%3E26014%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14370671&rft_id=info:pmid/3858855&rft_jstor_id=26014&rfr_iscdi=true |