Laser light-scattering study of the toxic effects of methylmercury on sperm motility
An in vitro study was designed using the laser light‐scattering technique to obtain further information on the dose‐effect relationship of methylmercury on sperm motility. The technique provided a quantitative evaluation of sperm swimming speed. Semen samples were collected from normal male Macaca f...
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| Veröffentlicht in: | J. Androl.; (United States) 1986-01, Vol.7 (1), p.11-15 |
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| creator | Mohamed, M. K Lee, W. I Mottet, N. K Burbacher, T. M |
| description | An in vitro study was designed using the laser light‐scattering technique to obtain further information on the dose‐effect relationship of methylmercury on sperm motility. The technique provided a quantitative evaluation of sperm swimming speed. Semen samples were collected from normal male Macaca fascicularis monkeys by anal electroejaculation. Methylmercury was added to aliquots of sperm suspensions in BWW medium in doses of 10, 5, 2, and 1 ppm. After 3 hours, the relative speed was 35%, 59%, 69%, and 92% of the corresponding controls at doses of 10, 5, 2, and 1 ppm, respectively. The percentage of motile spermatozoa decreased significantly at 10 ppm. By microscopic observation abnormal motility was detected at 5 and 10 ppm, especially after 20 to 40 minutes. Head movement increased from side to side, and many spermatozoa developed coiled tails. The technique proved useful for defining the dose‐effect relationship of methylmercury and sperm swimming speed. |
| doi_str_mv | 10.1002/j.1939-4640.1986.tb00858.x |
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K ; Lee, W. I ; Mottet, N. K ; Burbacher, T. M</creator><creatorcontrib>Mohamed, M. K ; Lee, W. I ; Mottet, N. K ; Burbacher, T. M ; Univ. of Washington, Seattle</creatorcontrib><description>An in vitro study was designed using the laser light‐scattering technique to obtain further information on the dose‐effect relationship of methylmercury on sperm motility. The technique provided a quantitative evaluation of sperm swimming speed. Semen samples were collected from normal male Macaca fascicularis monkeys by anal electroejaculation. Methylmercury was added to aliquots of sperm suspensions in BWW medium in doses of 10, 5, 2, and 1 ppm. After 3 hours, the relative speed was 35%, 59%, 69%, and 92% of the corresponding controls at doses of 10, 5, 2, and 1 ppm, respectively. The percentage of motile spermatozoa decreased significantly at 10 ppm. By microscopic observation abnormal motility was detected at 5 and 10 ppm, especially after 20 to 40 minutes. Head movement increased from side to side, and many spermatozoa developed coiled tails. The technique proved useful for defining the dose‐effect relationship of methylmercury and sperm swimming speed.</description><identifier>ISSN: 0196-3635</identifier><identifier>EISSN: 1939-4640</identifier><identifier>DOI: 10.1002/j.1939-4640.1986.tb00858.x</identifier><identifier>PMID: 3944015</identifier><identifier>CODEN: JOAND3</identifier><language>eng</language><publisher>Oxford, UK: Am Soc Andrology</publisher><subject>560305 - Chemicals Metabolism & Toxicology- Vertebrates- (-1987) ; ANIMALS ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; Chemical and industrial products toxicology. Toxic occupational diseases ; Dose-Response Relationship, Drug ; DOSE-RESPONSE RELATIONSHIPS ; GERM CELLS ; Infertility, Male - chemically induced ; Kinetics ; laser light‐scattering ; LASERS ; LIGHT SCATTERING ; Macaca fascicularis ; Macaca fasicularis ; Male ; MAMMALS ; Medical sciences ; MERCURY COMPOUNDS ; Metals and various inorganic compounds ; METHYLMERCURY ; Methylmercury Compounds - pharmacology ; Methylmercury Compounds - toxicity ; MONKEYS ; ORGANIC COMPOUNDS ; ORGANIC MERCURY COMPOUNDS ; PRIMATES ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; SCATTERING ; Scattering, Radiation ; Sperm Motility - drug effects ; SPERMATOCYTES ; spermatozoa motility ; Toxicology ; VERTEBRATES ; VIABILITY</subject><ispartof>J. Androl.; (United States), 1986-01, Vol.7 (1), p.11-15</ispartof><rights>1986 American Society of Andrology</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5518-b1f07ea8e1b945b971ad78344310bb5d61a69967d07e08815d03a0c363ce59663</citedby><cites>FETCH-LOGICAL-c5518-b1f07ea8e1b945b971ad78344310bb5d61a69967d07e08815d03a0c363ce59663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8709795$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3944015$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5842806$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohamed, M. K</creatorcontrib><creatorcontrib>Lee, W. I</creatorcontrib><creatorcontrib>Mottet, N. K</creatorcontrib><creatorcontrib>Burbacher, T. M</creatorcontrib><creatorcontrib>Univ. of Washington, Seattle</creatorcontrib><title>Laser light-scattering study of the toxic effects of methylmercury on sperm motility</title><title>J. Androl.; (United States)</title><addtitle>J Androl</addtitle><description>An in vitro study was designed using the laser light‐scattering technique to obtain further information on the dose‐effect relationship of methylmercury on sperm motility. The technique provided a quantitative evaluation of sperm swimming speed. Semen samples were collected from normal male Macaca fascicularis monkeys by anal electroejaculation. Methylmercury was added to aliquots of sperm suspensions in BWW medium in doses of 10, 5, 2, and 1 ppm. After 3 hours, the relative speed was 35%, 59%, 69%, and 92% of the corresponding controls at doses of 10, 5, 2, and 1 ppm, respectively. The percentage of motile spermatozoa decreased significantly at 10 ppm. By microscopic observation abnormal motility was detected at 5 and 10 ppm, especially after 20 to 40 minutes. Head movement increased from side to side, and many spermatozoa developed coiled tails. The technique proved useful for defining the dose‐effect relationship of methylmercury and sperm swimming speed.</description><subject>560305 - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)</subject><subject>ANIMALS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Dose-Response Relationship, Drug</subject><subject>DOSE-RESPONSE RELATIONSHIPS</subject><subject>GERM CELLS</subject><subject>Infertility, Male - chemically induced</subject><subject>Kinetics</subject><subject>laser light‐scattering</subject><subject>LASERS</subject><subject>LIGHT SCATTERING</subject><subject>Macaca fascicularis</subject><subject>Macaca fasicularis</subject><subject>Male</subject><subject>MAMMALS</subject><subject>Medical sciences</subject><subject>MERCURY COMPOUNDS</subject><subject>Metals and various inorganic compounds</subject><subject>METHYLMERCURY</subject><subject>Methylmercury Compounds - pharmacology</subject><subject>Methylmercury Compounds - toxicity</subject><subject>MONKEYS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC MERCURY COMPOUNDS</subject><subject>PRIMATES</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>SCATTERING</subject><subject>Scattering, Radiation</subject><subject>Sperm Motility - drug effects</subject><subject>SPERMATOCYTES</subject><subject>spermatozoa motility</subject><subject>Toxicology</subject><subject>VERTEBRATES</subject><subject>VIABILITY</subject><issn>0196-3635</issn><issn>1939-4640</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkctu3CAUQFHVKp1O-wmVrLTqzlMYjA3dRFH6SjRqNskaYXwdM8L2FLA8_vti2Zp9V6B7z31wQOia4B3BeP_1uCOCijTLsxgQPN-FEmPO-O78Cm0uqddog4nIU5pT9ha98_4YazEp6BW6oiLLMGEb9HRQHlxizUsTUq9VCOBM95L4MFRT0tdJaCAJ_dnoBOoadPBzsIXQTLYFpwcXqS7xJ3Bt0vbBWBOm9-hNrayHD-u5Rc8_fzzd_U4Pj7_u724PqWaM8LQkNS5AcSClyFgpCqKqgtMsowSXJatyonIh8qKKFOacsApThXV8jwYm8pxu0fXSt_fBSK9NAN3ovuvinpLxbM_xDH1ZoJPr_w7gg2yN12Ct6qAfvCQZZWIftWzRtwXUrvfeQS1PzrTKTZJgOXuXRznLlbNcOXuXq3d5jsUf1ylD2UJ1KV1Fx_znNa-iZVs71WnjLxgvsCjEjN0s2GgsTP-xgHy4_fM93mKHT0uHJn7paBxI3ypr41pEjuNYSCIJof8AG1Or1Q</recordid><startdate>198601</startdate><enddate>198601</enddate><creator>Mohamed, M. K</creator><creator>Lee, W. I</creator><creator>Mottet, N. K</creator><creator>Burbacher, T. M</creator><general>Am Soc Andrology</general><general>Blackwell Publishing Ltd</general><general>American Society of Andrology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>OTOTI</scope></search><sort><creationdate>198601</creationdate><title>Laser light-scattering study of the toxic effects of methylmercury on sperm motility</title><author>Mohamed, M. K ; Lee, W. I ; Mottet, N. K ; Burbacher, T. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5518-b1f07ea8e1b945b971ad78344310bb5d61a69967d07e08815d03a0c363ce59663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>560305 - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)</topic><topic>ANIMALS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Dose-Response Relationship, Drug</topic><topic>DOSE-RESPONSE RELATIONSHIPS</topic><topic>GERM CELLS</topic><topic>Infertility, Male - chemically induced</topic><topic>Kinetics</topic><topic>laser light‐scattering</topic><topic>LASERS</topic><topic>LIGHT SCATTERING</topic><topic>Macaca fascicularis</topic><topic>Macaca fasicularis</topic><topic>Male</topic><topic>MAMMALS</topic><topic>Medical sciences</topic><topic>MERCURY COMPOUNDS</topic><topic>Metals and various inorganic compounds</topic><topic>METHYLMERCURY</topic><topic>Methylmercury Compounds - pharmacology</topic><topic>Methylmercury Compounds - toxicity</topic><topic>MONKEYS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC MERCURY COMPOUNDS</topic><topic>PRIMATES</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>SCATTERING</topic><topic>Scattering, Radiation</topic><topic>Sperm Motility - drug effects</topic><topic>SPERMATOCYTES</topic><topic>spermatozoa motility</topic><topic>Toxicology</topic><topic>VERTEBRATES</topic><topic>VIABILITY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohamed, M. K</creatorcontrib><creatorcontrib>Lee, W. I</creatorcontrib><creatorcontrib>Mottet, N. K</creatorcontrib><creatorcontrib>Burbacher, T. M</creatorcontrib><creatorcontrib>Univ. of Washington, Seattle</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>OSTI.GOV</collection><jtitle>J. Androl.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohamed, M. K</au><au>Lee, W. I</au><au>Mottet, N. K</au><au>Burbacher, T. M</au><aucorp>Univ. of Washington, Seattle</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laser light-scattering study of the toxic effects of methylmercury on sperm motility</atitle><jtitle>J. Androl.; (United States)</jtitle><addtitle>J Androl</addtitle><date>1986-01</date><risdate>1986</risdate><volume>7</volume><issue>1</issue><spage>11</spage><epage>15</epage><pages>11-15</pages><issn>0196-3635</issn><eissn>1939-4640</eissn><coden>JOAND3</coden><abstract>An in vitro study was designed using the laser light‐scattering technique to obtain further information on the dose‐effect relationship of methylmercury on sperm motility. The technique provided a quantitative evaluation of sperm swimming speed. Semen samples were collected from normal male Macaca fascicularis monkeys by anal electroejaculation. Methylmercury was added to aliquots of sperm suspensions in BWW medium in doses of 10, 5, 2, and 1 ppm. After 3 hours, the relative speed was 35%, 59%, 69%, and 92% of the corresponding controls at doses of 10, 5, 2, and 1 ppm, respectively. The percentage of motile spermatozoa decreased significantly at 10 ppm. By microscopic observation abnormal motility was detected at 5 and 10 ppm, especially after 20 to 40 minutes. Head movement increased from side to side, and many spermatozoa developed coiled tails. The technique proved useful for defining the dose‐effect relationship of methylmercury and sperm swimming speed.</abstract><cop>Oxford, UK</cop><pub>Am Soc Andrology</pub><pmid>3944015</pmid><doi>10.1002/j.1939-4640.1986.tb00858.x</doi><tpages>5</tpages></addata></record> |
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| issn | 0196-3635 1939-4640 |
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| subjects | 560305 - Chemicals Metabolism & Toxicology- Vertebrates- (-1987) ANIMALS Biological and medical sciences BIOLOGICAL EFFECTS Chemical and industrial products toxicology. Toxic occupational diseases Dose-Response Relationship, Drug DOSE-RESPONSE RELATIONSHIPS GERM CELLS Infertility, Male - chemically induced Kinetics laser light‐scattering LASERS LIGHT SCATTERING Macaca fascicularis Macaca fasicularis Male MAMMALS Medical sciences MERCURY COMPOUNDS Metals and various inorganic compounds METHYLMERCURY Methylmercury Compounds - pharmacology Methylmercury Compounds - toxicity MONKEYS ORGANIC COMPOUNDS ORGANIC MERCURY COMPOUNDS PRIMATES RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT SCATTERING Scattering, Radiation Sperm Motility - drug effects SPERMATOCYTES spermatozoa motility Toxicology VERTEBRATES VIABILITY |
| title | Laser light-scattering study of the toxic effects of methylmercury on sperm motility |
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